数字农科院2.0

Host-Induced Gene Silencing of an Adenylate Kinase Gene Involved in Fungal Energy Metabolism Improves Plant Resistance to Verticillium dahliae

文献类型： 外文期刊

作者： Xiaofeng Su; Guoqing Lu; Xiaokang Li; Guoqing Sun; 郭惠明;; Hongmei Cheng

作者机构：

关键词： Host-Induced Gene Silencing (Higs); Verticillium Dahliae; Pathogenicity Factor; Adenylate Kinase

期刊名称： Biomolecules

ISSN： 2218-273X

年卷期： 2020 年 10 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： Verticillium wilt, caused by the ascomycete fungus Verticillium dahliae (Vd), is a devastating disease of numerous plant species. However, the pathogenicity/virulence-related genes in this fungus, which may be potential targets for improving plant resistance, remain poorly elucidated. For the study of these genes in Vd, we used a well-established host-induced gene silencing (HIGS) approach and identified 16 candidate genes, including a putative adenylate kinase gene (VdAK). Transiently VdAK-silenced plants developed milder wilt symptoms than control plants did. VdAK-knockout mutants were more sensitive to abiotic stresses and had reduced germination and virulence on host plants. Transgenic Nicotiana benthamiana and Arabidopsis thaliana plants that overexpressed VdAK dsRNAs had improved Vd resistance than the wild-type. RT-qPCR results showed that VdAK was also crucial for energy metabolism. Importantly, in an analysis of total small RNAs from Vd strains isolated from the transgenic plants, a small interfering RNA (siRNA) targeting VdAK was identified in transgenic N. benthamiana. Our results demonstrate that HIGS is a promising strategy for efficiently screening pathogenicity/virulence-related genes of Vd and that VdAK is a potential target to control this fungus.

分类号：

  • 相关文献

[1]Host-induced gene silencing of the Verticillium dahliae thiamine transporter protein gene (VdThit) confers resistance to Verticillium wilt in cotton. Qi Wang,Guoqiang Pan,Xingfen Wang,Zhengwen Sun,Huiming Guo,Xiaofeng Su,Hongmei Cheng. 2024

[2]Discovery of an SQS-PSY Domain-Containing Protein in Meloidogyne incognita Reveals Its Function in Parasitism. Junru Lu,Runmao Lin,Yunlong Ma,Xin Sun,Yang Jiao,Xinyue Cheng,Bingyan Xie. 2025

[3]The Cotton Ghwin2 Gene Activates T.he Cuticle Biosynthesis Pathway A nd Influences The Salicylic And Jasmonic Acid Biosynthesis Pathways. Ge, Xiaoyang,Liu, Di,Hou, Yuxia,Li, Fuguang,Ma, Xiaowen,Wang, Ping,Liu, Nana,Sun, Yun,Pei, Yakun,Li, Xiancai. 2019

[4]Ghsnap33, A T-Snare Protein From G.ossypium Hirsutum, Mediates Resistance T o Verticillium Dahliae Infection And Tolerance To Drought Stress. Li, Xiancai,Sun, Yun,Pei, Yakun,Hou, Yuxia,Wang, Ping,Zhang, Xueyan,Li, Fuguang. 2018

[5]Mutation Of Key Amino Acids I.n The Polygalacturonase-Inhibiting Proteins C kpgip1 And Ghpgip1 Improves Resistance To Verticillium Wilt In Cotton. Sun, Yun,Li, Xiancai,Pei, Yakun,Liu, Nana,Wang, Ping,Li, Fuguang,Duan, Hongxia,Ge, Xiaoyang,Hou, Yuxia. 2018

[6]Rna-Sequencing, Physiological And Rnai Analyses P.rovide Insights Into The R esponse Mechanism Of The Abc-Mediated Resistance To Verticillium Dahliae Infection In Cotton. Peng, Renhai,Magwanga, Richard Odongo,Lu, Pu,Wang, Kunbo,Wang, Xingfen,Zhou, Zhongli,Xu, Yanchao,Wang, Xingxing,Cai, Xiaoyan,Dong, Qi,Dong, Qi,Hou, Yuqing,Magwanga, Richard Odongo,Liu, Fang,Kirungu, Joy Nyangasi,Ma, Zhiying. 2019

[7]A Cotton Cyclin-Dependent Kinase E C.onfers Resistance To Verticillium D ahliae Mediated By Jasmonate-Responsive Pathway. Liu, Di,Wang, Ping,Li, Xiancai,Pei, Yakun,Hou, Yuxia,Liu, Nana,Sun, Yun,Ge, Xiaoyang,Li, Fuguang. 2018

[8]Freb Is Involved In The F.erric Metabolism And Multiple P athogenicity-Related Traits Of Verticillium Dahliae. Cheng, Hongmei,Li, Xiaokang,Guo, Huiming,Qi, Xiliang,Su, Xiaofeng,Rehman, Latifur. 2018

[9]Cultivar-Dependent Variation Of The Cotton Rhizosphere And Endosphere Microbiome Under Field Conditions. Xu, Xiangming,Shi, Yongqiang,Wei, Feng,Feng, Hongjie,Zhu, Heqin,Zhao, Lihong,Feng, Hongjie,Deakin, Greg,Wei, Feng,Feng, Zili,Zhu, Heqin. 2019

[10]Functional Characterization Of Target Of R.apamycin Signaling In Verticillium D ahliae. Zhuo, Fengping,Song, Yun,Feng, Li,Zhu, Tingting,Zhuo, Fengping,Li, Linxuan,Ren, Maozhi,Luo, Xiumei,Song, Yun,Li, Fuguang,Li, Fuguang. 2019

[11]Melatonin Enhances Cotton Immunity To V.erticillium Wilt Via Manipulating L ignin And Gossypol Biosynthesis. He, Qiuling,Zhang, Yi,Xie, Qianwen,Chen, Jinhong,Zhu, Shuijin,Zhang, Fan,Yu, Jingwen,Mei, Lei,Su, Bangrong,Zhao, Tianlun,Xie, Qianwen,Zhang, Fan,Li, Cong,Li, Cheng,Li, Cheng,Chen, Jinhong,Zhu, Shuijin,Zhao, Tianlun,Li, Cong,Yu, Jingwen,Zhang, Yi,Su, Bangrong,Mei, Lei. 2019

[12]Simultaneous Editing Of Two Copies O.f Gh14-3-3D Confers Enhanced T ransgene-Clean Plant Defense Against Verticillium Dahliae In Allotetraploid Upland Cotton. Fan, Qiang,Li, Fuguang,Wang, Peng,Wu, Jiahe,Xiao, Juanli,Zhang, Zhennan,Luo, Xiaoli,Hu, Guang,Wang, Peng,Ge, Xiaoyang. 2018

[13]Dynamic Infection Ofverticillium Dahliaein Upland Cotton. Hu, H. -L.,Tang, C. -M.,Yang, Y. -H.,Zhao, M. -W.,Zhang, C. -J.,Zhu, H. -Q.,Wang, X. -N.,Shi, L.,Zhang, J.. 2020

[14]Ghabp19, A Novel Germin-Like Protein F.rom Gossypium Hirsutum, Plays A n Important Role In The Regulation Of Resistance To Verticillium And Fusarium Wilt Pathogens. Liu, Nana,Ge, Xiaoyang,Li, Xiancai,Hou, Yuxia,Jia, Yujiao,Li, Fuguang,Pei, Yakun,Sun, Yun,Zhu, Yutao. 2019

[15]Population Genomics Demystifies The Defoliation P.henotype In The Plant P athogen Verticillium Dahliae. Zhang, DD, Wang, J, Wang, D, Kong, ZQ, Zhou, L, Zhang, GY, Gui, YJ, Li, JJ, Huang, JQ, Wang, BL, Liu, C, Yin, CM, Li, RX, Li, TG, Wang, JL, Short, DPG, Klosterman, SJ, Bostock, RM, Subbarao, KV, Chen, JY, Dai, XF. 2019

[16]Two Lysin-Motif Receptor Kinases, Gh-Lyk1 A.nd Gh-Lyk2, Contribute To R esistance Against Verticillium Wilt In Upland Cotton. Gu, Zhouhang,Ye, Fei,Wang, Qian,Qian, Shasha,Li, Fangfang,Gu, Zhouhang,Zhou, Xueping,Chen, Tianzi,Liu, Tingli,Yang, Yuwen,Wang, Jinyan,Zhang, Baolong,Ding, Bo,Wang, Guoliang,Zhou, Xueping. 2017

[17]Pectin lyase enhances cotton resistance to Verticillium wilt by inducing cell apoptosis of Verticillium dahliae. 张朝军,,朱荷琴. 2021

[18]GhPLP2 Positively Regulates Cotton Resistance to Verticillium Wilt by Modulating Fatty Acid Accumulation and Jasmonic Acid Signaling Pathway. Zhu, Yutao,Hu, Xiaoqian,Wang, Ping,Gao, Linying,Pei, Yakun,Ge, Zhaoyue,Ge, Xiaoyang,Li, Fuguang,Hou, Yuxia. 2021

[19]Functional Analyses Of Small Secreted Cysteine-Rich Proteins Identified Candidate Effectors In Verticillium Dahliae. Wang, D, Tian, L, Zhang, DD, Song, J, Song, SS, Yin, CM, Zhou, L, Liu, Y, Wang, BL, Kong, ZQ, Klosterman, SJ, Li, JJ, Wang, J, Li, TG, Adamu, S, Subbarao, KV, Chen, JY, Dai, XF. 2020

[20]Verticillium Dahliae Pevd1, An Alt A. 1-Like Protein, Targets C otton Pr5-Like Protein And Promotes Fungal Infection. Zhang, Yi,Liang, Yingbo,Dong, Yijie,Yang, Xiufen,Yang, Xiufen,Qiu, Dewen,Gao, Yuhan,Qiu, Dewen. 2019

作者其他论文 更多>>