数字农科院2.0

Cell Membrane-Interrupting Antimicrobial Peptides From Isatis Indigotica Fortune Isolated By A Bacillus Subtilis Expression System

文献类型： 外文期刊

作者： 刘允军;;王国英

作者机构：

关键词： Antimicrobial Peptide; Plant Resistance Gene; Membrane-Interrupting Peptides; Gene Expression System; Bacillus Subtilis; Isatis Indigotica

期刊名称： BIOMOLECULES

ISSN： 2218-273x

年卷期： 2020 年 10 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： The situation of drug resistance has become more complicated due to the scarcity of plant resistance genes, and overcoming this challenge is imperative. Isatis indigotica has been used for the treatment of wounds, viral infections, and inflammation for centuries. Antimicrobial peptides (AMPs) are found in all classes of life ranging from prokaryotes to eukaryotes. To identify AMPs, I. indigotica was explored using a novel, sensitive, and high-throughput Bacillus subtilis screening system. We found that IiR515 and IiR915 exhibited significant antimicrobial activities against a variety of bacterial (Xanthomonas oryzae, Ralstonia solanacearum, Clavibacter michiganensis, and C. fangii) and fungal (Phytophthora capsici and Botrytis cinerea) pathogens. Scanning electron microscope and cytometric analysis revealed the possible mechanism of these peptides, which was to target and disrupt the bacterial cell membrane. This model was also supported by membrane fluidity and electrical potential analyses. Hemolytic activity assays revealed that these peptides may act as a potential source for clinical medicine development. In conclusion, the plant-derived novel AMPs IiR515 and IiR915 are effective biocontrol agents and can be used as raw materials in the drug discovery field.

分类号：

  • 相关文献

[1]Synergistic Effects Of The Combined A.pplication Of Bacillus Subtilis H 158 And Strobilurins For Rice Sheath Blight Control. Huang, Shiwen,Hou, Yuxuan,Liu, Lianmeng,Xu, Yihua,Sun, Lei,Li, Lu,Sun, Lei,Gao, Jian,Liu, Lianmeng,Xu, Yihua,Huang, Shiwen,Wang, Ling,Liang, Mengqi,Li, Lu. 2018

[2]An Enhanced Vector-Free Allele Exchange (.Vfae) Mutagenesis Protocol For G enome Editing In A Wide Range Of Bacterial Species. Yan, Yongliang,Shang, Liguo,Yang, Zhimin,Zhang, Chen,Zhan, Yuhua,Gomaa, Ahmed E.,Deng, Zhiping,Jiang, Shijie,Lu, Wei,Lin, Min. 2017

[3]Effects Of Long-Term Bacillus Subtilis C.gmcc 1.921 Supplementation On P erformance, Egg Quality, And Fecal And Cecal Microbiota Of Laying Hens. Guo, JR, Dong, XF, Liu, S, Tong, JM. 2017

[4]Isolation And Identification Of A B.acillus Subtilis Hz-72 Exhibiting B iocontrol Activity Against Flax Seedling Blight. Tan, TM, Zhu, JX, Shen, AR, Li, JL, Yu, YT, Zhang, MJ, Zhao, MR, Li, ZM, Chen, J, Gao, CS, Cheng, Y, Guo, LT, Yan, L, Sun, XP, Zeng, LB, Yan, Z. 2019

[5]Transcriptional Profile Of Gene Clusters I.nvolved In The Methylerythritol P hosphate Pathway In Bacillus Subtilis 916. Xiao Ya-jing,Xiao Ya-jing,Peng Qi,Gao Tan-tan,Zhang Jie,Sun Dong-mei,Song Fu-ping. 2019

[6]Characterization Of Subtilin L-Q11, A N.ovel Class I Bacteriocin S ynthesized By Bacillus Subtilis L-Q11 Isolated From Orchard Soil. Qin, YX, Wang, Y, He, YH, Zhang, Y, She, QX, Chai, YR, Lp, PL, Shang, QM. 2019

[7]Seed Treatment Containing Bacillus Subtilis B.y-2 In Combination With O ther Bacillus Isolates For Control Of Sclerotinia Sclerotiorum On Oilseed Rape. Hu, XJ, Roberts, DP, Xie, LH, Qin, L, Li, YS, Liao, XS, Han, PP, Yu, CB, Liao, X. 2019

[8]Engineering Of A Bacillus Subtilis S.train Deficient In Cellulase: A pplication In Degumming Of Ramie. Yang, Q, Duan, SW, Cheng, LF, Feng, XY, Zheng, K, Xie, CL, Liu, ZY, Pen, YD. 2019

[9]Extracellular Production Of Active-Form Streptomyces Mobaraensis Transglutaminase In Bacillus Subtilis. Fu, LH, Wang, Y, Ju, JS, Cheng, L, Xu, YQ, Yu, B, Wang, LM. 2020

[10]In Vitro Efficacy Of Microbial A.ntagonists, Botanical Extracts And S ynthetic Chemicals Against Mango Quick Wilt Pathogen Ceratocystis Manginecans. Ahmad, T, Moosa, A, Rehman, A. 2019

[11]Potent Fungal Chitinase for the Bioconversion of Mycelial Waste. Liu, Tian,Liu, Tian,Yang, Qing,Yang, Qing,Han, Hongyu,Zhou, Yong,Han, Hongyu,Wang, Di,Guo, Xiaoguang,Fukamizo, Tamo,Yang, Qing. 2020

[12]Improving The Production Of Ahl Lactonase Aiio-Aio6 Fromochrobactrumsp. M231 In Intracellular Protease-Deficientbacillus Subtilis. Xia, R, Yang, YL, Pan, XL, Gao, CC, Yao, YY, Liu, XW, Teame, T, Zhang, FL, Hu, J, Ran, C, Zhang, Z, Liu-Clarke, JH, Zhou, ZG. 2020

[13]Biological Characteristics Of A New A.ntibacterial Peptide And Its A ntibacterial Mechanisms Against Gram-Negative Bacteria. Pei, Z.,Ying, X.,Tang, Y.,Kong, L.,Ma, H.,Liu, S.,Zhang, H.,Liu, L.,Wang, K.,Zhang, D.,Gao, Y.. 2018

[14]A novel PCR-based method for high throughput prokaryotic expression of antimicrobial peptide genes. Ke, Tao,Chen, Jibao,Liang, Su,Huang, Jin,Ma, Xiangdong,Kang, Jianxiong,Liu, Dongqi,Ke, Tao,Mao, Han,Dong, Caihua,Huang, Junyan,Liu, Shengyi. 2012

[15]Functional analysis of a novel cysteine-rich antimicrobial peptide from the salivary glands of the tick Rhipicephalus haemaphysaloides. Zhang, Houshuang,Yang, Siqi,Gong, Haiyan,Cao, Jie,Zhou, Yongzhi,Zhou, Jinlin,Zhou, Jinlin,Yang, Siqi.

[16]Sequence characterization and expression patterns of two defensin-like antimicrobial peptides from the tick Haemaphysalis longicornis. Zhou, Jinlin,Liao, Min,Ueda, Marni,Gong, Haiyan,Xuan, Xuenan,Fujisaki, Kozo. 2007

[17]Expression of antimicrobial peptide LH multimers in Escherichia coli C43(DE3). Tian, Zi-gang,Dong, Tian-tang,Yang, Ya-lin,Teng, Da,Wang, Jian-hua.

[18]Two novel antimicrobial peptides purified from the symbiotic bacteria Xenorhabdus budapestensis NMC-10. Xiao, Yao,Meng, Fanlu,Qiu, Dewen,Yang, Xiufen.

[19]Recombinant production of the antimicrobial peptide NZ17074 in Pichia pastoris using SUMO3 as a fusion partner. Wang, X. J.,Wang, X. M.,Teng, D.,Zhang, Y.,Mao, R. Y.,Wang, J. H.,Wang, X. J.,Wang, X. M.,Teng, D.,Zhang, Y.,Mao, R. Y.,Wang, J. H..

[20]Identification of a cysteine-rich antimicrobial peptide from salivary glands of the tick Rhipicephalus haemaphysaloides. Zhang, Houshuang,Zhang, Wenjie,Wang, Xinzhi,Zhou, Yongzhi,Wang, Na,Zhou, Jinlin. 2011

作者其他论文 更多>>