数字农科院2.0

The Small Gtpase Rab5A And Its Guanine Nucleotide Exchange Factors Are Involved In Post-Golgi Trafficking Of Storage Proteins In Developing Soybean Cotyledon

文献类型: 外文期刊

作者: Wei, ZY; Pan, T; Zhao, YY; Su, BH; Ren, YL; Qiu, LJ

作者机构:

关键词: Guanine Nucleotide Exchange Factor; Protein Storage Vacuole; Rab5; Soybean; Transient Expression; Vps9

期刊名称: JOURNAL OF EXPERIMENTAL BOTANY

ISSN: 0022-0957

年卷期: 2020 年 71 卷 3 期

页码:

收录情况: JCR(2021版)

摘要: Storage protein is the most abundant nutritional component in soybean seed. Morphology-based evidence has verified that storage proteins are initially synthesized on the endoplasmic reticulum, and then follow the Golgi-mediated pathway to the protein storage vacuole. However, the molecular mechanisms of storage protein trafficking in soybean remain unknown. Here, we clone the soybean homologs of Rab5 and its guanine nucleotide exchange factor (GEF) VPS9. GEF activity combined with yeast two-hybrid assays demonstrated that GmVPS9a2 might specifically act as the GEF of the canonical Rab5, while GmVPS9b functions as a common activator for all Rab5s. Subcellular localization experiments showed that GmRab5a was dually localized to the trans-Golgi network and pre-vacuolar compartments in developing soybean cotyledon cells. Expression of a dominant negative variant of Rab5a, or RNAi of either Rab5a or GmVPS9s, significantly disrupted trafficking of mRFP-CT10, a cargo marker for storage protein sorting, to protein storage vacuoles in maturing soybean cotyledons. Together, our results systematically revealed the important role of GmRab5a and its GEFs in storage protein trafficking, and verified the transient expression system as an efficient approach for elucidating storage protein trafficking mechanisms in seed.

分类号:

  • 相关文献

[1]Guanine nucleotide exchange factor 2 for Rab5 proteins coordinated with GLUP6/GEF regulates the intracellular transport of the proglutelin from the Golgi apparatus to the protein storage vacuole in rice endosperm. Wen, Liuying,Fukuda, Masako,Ishino, Sonoko,Ishino, Yoshizumi,Kumamaru, Toshihiro,Wen, Liuying,Sunada, Mariko,Ueda, Takashi,Okita, Thomas W.,Ogawa, Masahiro,Ueda, Takashi.

[2]Gmgpa3 Is Involved In Post-Golgi Trafficking Of Storage Proteins And Cell Growth In Soybean Cotyledons. Wei, ZY, Chen, Y, Zhang, B, Ren, YL, Qiu, LJ. 2020

[3]Structural And Functional Analysis Of A. Bidirectional Promoter From G ossypium Hirsutum In Arabidopsis. Yang, JT, Wang, XJ, Hasi, A, Wang, ZX. 2018

[4]A Versatile Vector Toolkit For F.unctional Analysis Of Rice G enes. He, Feng,Wang, Guo-Liang,He, Feng,Sun, Wenxian,Ning, Yuese,Zhang, Fan,Wang, Guo-Liang. 2018

[5]Molecular And Biochemical Analysis Of T.he Castor Caruncle Reveals A Set Of Unique Genes Involved In Oil Accumulation In Non-Seed Tissues. Wan, X, Liu, Q, Dong, B, Pillai, SV, Huang, FH, Singh, SP, Zhou, XR. 2019

[6]Efficient CELI endonuclease production in Nicotiana benthamiana through transient expression and applications in detections of mutation and gene editing events. Yao, Xue-Feng,Yao, Xue-Feng,Liu, Chun-Ming,Liu, Chun-Ming,Guo, Lei,Wu, Shengyang. 2020

[7]The Evolution And Biocatalysis Of F.ad2 Indicate Its Correlation T o The Content Of Seed Oil In Plants. Chen, Peng,Peng, Li,Wei, Lei,Wang, Wenyi,Wang, Zhao,Yuan, Fengjie,Zhao, Man,Ying, Xiangxian,Qin, Zhen,Zhao, Man. 2019

[8]The Roles Of Gmerf135 In I.mproving Salt Tolerance And D ecreasing Aba Sensitivity In Soybean. Zhao, Meng-Jie,Xu, Zhao-Shi,Xu, Zhao-Shi,Zheng, Jia-Cheng,Yin, Li-Juan,Yin, Li-Juan,Xu, Zhao-Shi,Chen, Ming,Fu, Jin-Dong,Lan, Jin-Hao,Wang, Yan-Xia,Ma, You-Zhi,Ma, Jian. 2019

[9]Dynamic Evolution Research And System I.mplementation Of International Soybean T rade Network Based On Complex Network. Dong, Chao,Kou, Yuantao,Zhao, Ruixue,Xian, Guojian,Ye, Sa. 2018

[10]The Elongation Factor Gmef4 Is I.nvolved In The Response T o Drought And Salt Tolerance In Soybean. Xu, Zhao-Shi,Chen, Ming,Gao, Yuan,Fu, Jin-Dong,Ma, You-Zhi,Ma, Jian,Zheng, Jia-Cheng,Zhou, Yong-Bin,Chen, Jun. 2019

[11]The Wrky Transcription Factor Gmwrky12 C.onfers Drought And Salt T olerance In Soybean. Chen, Jun,Chen, Ming,Du, Yong-Tao,Jin, Long-Guo,Ma, Jian,Ma, You-Zhi,Min, Dong-Hong,Shi, Wen-Yan,Shi, Wen-Yan,Xu, Zhao-Shi,Zhou, Yong-Bin,Zhang, Xiao-Hong. 2018

[12]Identification And Characterization Of Gmmyb118 R.esponses To Drought And S alt Stress. Chen, Ming,Zhou, Yong-Bin,Wang, Chang-Tao,Xu, Zhao-Shi,Wang, Yan-Xia,Zhao, Meng-Jie,Ma, You-Zhi,Liu, Yong-Wei,Gao, Yuan,Du, Yong-Tao,Chen, Jun. 2018

[13]Soybean Hairy Roots Produced In V.itro By Agrobacterium Rhizogenes-Mediated T ransformation. Chen, Li,Cai, Yupeng,Liu, Xiujie,Guo, Chen,Hou, Wensheng,Chen, Li,Cai, Yupeng,Liu, Xiujie,Guo, Chen,Sun, Shi,Wu, Cunxiang,Jiang, Bingjun,Han, Tianfu,Hou, Wensheng. 2018

[14]Roles Of Soybean Plasma Membrane I.ntrinsic Protein Gmpip2;9 In D rought Tolerance And Seed Development. Liu, Ruifang,Shou, Huixia,Lu, Linghong,Wang, Chuang,Lu, Linghong,Dong, Changhe,Zhou, Bin,Wang, Chuang. 2018

[15]Effect Of Nitrogen Forms On R.eduction Of Manganese Oxides I n An Oxisol By Plant Root Exudates. Xu, Ren-kou,Zhou, Qin,Li, Zhong-yi,Liu, Yuan,Liu, Yuan,Deng, Kai-ying,Zhou, Qin,Liu, Yuan,Li, Zhong-yi. 2017

[16]Genome-Wide Analysis Of The Yabby F.amily In Soybean And F unctional Identification Of Gmyabby10 Involvement In High Salt And Drought Stresses. Chai, Shou-Cheng,Lu, Dan,Chen, Zhan-Yu,Ji, Yu-Jie,Zheng, Wei -Jun,Cui, Xi-Yan,Zhao, Shu-Ping,Yu, Tai-Fei,Zhang, Shuang-Xi,Zhao, Shu-Ping. 2017

[17]Dissipation Behavior And Risk Assessment O.f Butralin In Soybean A nd Soil Under Field Conditions. Li, Li,He, Yujian,Liu, Rong,Li, Congdi,Li, Wei,Li, Congdi,Yuan, Longfei. 2017

[18]Intercropping In Sugarcane Cultivation Influenced T.he Soil Properties And E nhanced The Diversity Of Vital Diazotrophic Bacteria. Wang, Fei-Yong,Wang, Fei-Yong,Solanki, Manoj Kumar,Yang, Li-Tao,Wang, Zhen,Wang, Fei-Yong,Singh, Rajesh Kumar,Li, Chang-Ning,Li, Chang-Ning,Lan, Tao-Ju,Singh, Pratiksha,Li, Yang-Rui,Li, Yang-Rui. 2017

[19]Evaluation Of Simple And Inexpensive H.igh-Throughput Methods For Phytic A cid Determination. Shu, Qingyao,Thavarajah, Pushparajah,Rasmussen, Soren K.,Shi, Jinrui,Zeder, Christophe,Zhou, Lan,Thavarajah, Pushparajah,Warkentin, Tom D.,Cichy, Karen,Hurrell, Richard F.,Bilyeu, Kristin,Brinch-Pedersen, Henrik,Raboy, Victor,Raboy, Victor,Johnson, Amy,Johnson, Amy. 2017

[20]Transcriptomic Analysis Of Genes In S.oybean In Response To P eronospora Manshurica Infection. Jia, Shirong,Wang, Xujing,Lin, Liping,Shi, Shuangfeng,Liu, Yuhui,Dong, Hang,Zhu, Sihui,Yu, Yue,Dong, Hang,Zhang, Chong,Wu, Yuanhua,Li, Mei,Tan, Jie,Zhu, Sihui. 2018

作者其他论文 更多>>