数字农科院2.0

Characterization Of Porcine Reproductive And Respiratory Syndrome Virus (Orf5 Rflp 1-7-4 Viruses) In Northern China

文献类型: 外文期刊

作者: Xie, CZ; Ha, Z; Nan, FL; Zhang, Y; Zhang, H; Li, JF; Zhang, P; Han, JC; Zhang, H; Zhuang, XY; Zhang, JY; Lu, HJ; Jin, NY

作者机构:

关键词: Genetic Variation; Prrsv; Virulence; Whole Genome Sequencing

期刊名称: MICROBIAL PATHOGENESIS

ISSN: 0882-4010

年卷期: 2020 年 140 卷

页码:

收录情况: JCR(2021版)

摘要: The porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of porcine reproductive and respiratory syndrome (PRRS). Disease outbreaks caused by NADC30-like PRRSV strains were a bit prevalent in China in recent years. In the present study, two newly emerged PRRSV strains, which were designated as PRRSV-ZDXYL-China-2018-1 and PRRSV-ZDXYL-China-2018-2 strains were found from piglets' lung tissues in Northern China. The virus belongs to lineage 1 of the PRRSV genotype 2 and is closely related to US strains that possess the open reading frame (OAFS) restriction fragment length polymorphism (RFLP) 1-7-4. The two strains were identified from infected weaning piglet herds in Zhaodong City, Heilongjiang province of China. The complete genome of the PRRSV-ZDXYL-China-2018-1 and PRRSV-ZDXYL-China-2018-2 strains were 15093 nt and 15110 nt, and shared 96.7%-97.0% and 97.1%-97.4% similarities with the US identified, ISU10 and NADC34 strains respectively. Then the PRRSV-ZDXYL-China-2018-1 strain was successfully isolated from the clinical sample. Our results demonstrate, that the emergence of ORF5 RFLP 1-7-4-like PRRSVs in China, could pose a significant challenge to PRRSV epidemic prevention.

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