数字农科院2.0

Gold Nanobones Enhanced Ultrasensitive Surface-Enhanced Raman Scattering Aptasensor For Detecting Escherichia Coil O157:H7

文献类型： 外文期刊

作者： Zhou, SS; Lu, C; Li, YZ; Xue, L; Zhao, CY; Tian, GF; Bao, YM; Tang, LH; Lin, JH; Zheng, JK

作者机构：

关键词： Sers; E. Coli O157:H7; Aptasensor; Gold Nanobone; Detection

期刊名称： ACS SENSORS

ISSN： 2379-3694

年卷期： 2020 年 5 卷 2 期

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： Sensitive, robust, and highly specific detection of Escherichia colt O157:H7, one of the most hazardous foodborne pathogens and the cause of numerous diseases, is needed to ensure public health. Herein, a one-pot step method is reported for the preparation of multifunctional gold nanobones (NBs) (GNR(Apt-1+RhB)) from gold nanorods (GNRs) comediated by an aptamer (Apt-1) and. the signal molecule rhodamine B (RhB) for surface-enhanced Raman scattering detection of E. coli O157:H7. The characterized result showed that Apt-1 and RhB were embedded in the gold NBs, and then, this combination exhibited good recognition, excellent stability, and significant Raman signal intensity enhancement. The Raman enhancement derived from a strong electromagnetic field distribution with the locations at the apex of both ends of the GNR(Apt-1+RhB) and the signal stability was because of the firm embedment of Apt-1 (poly A20 + E. coli O157:H7 aptamers) and RhB on the surface of the GNR(Apt-1+RhB). Optimization experiments established that surface-enhanced Raman-scattered RhB absorption at 1350 cm(-1) had a strong linear relationship (y = 180.30x - 61.49; R-2 = 0.9982) with E. coli O157:H7 concentration over the range of 10-10,000 cfu/mL with a limit of detection of 3 cfu/mL. This novel aptasensor sensitively detects E. coli O157:H7 and has great promise for food pathogenic bacteria detection.

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