数字农科院2.0

Competitive-Type Pressure-Dependent Immunosensor For Highly Sensitive Detection Of Diacetoxyscirpenol In Wheat Via Monoclonal Antibody

文献类型： 外文期刊

作者： Tang, XQ; Wu, J; Wu, WQ; Zhang, ZW; Zhang, WQ; Zhang, Q; Zhang, W; Chen, XM; Li, PW

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期刊名称： ANALYTICAL CHEMISTRY

ISSN： 0003-2700

年卷期： 2020 年 92 卷 5 期

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： Diacetoxyscirpenol (DAS) is a type A trichothecene mycotoxin with low molecular weight, and with respect to its toxicity and the occurrence in food and feed, it is known as a potential risk for public and animal health. In the present study, first, a sensitive and specific monoclonal antibody (SE7) was developed. Then, the antibody was applied to develop a competitive-type pressure-dependent immunosensor (CTPDI). The Au@PtNP was synthesized and labeled with goat antimouse antibody (Au@PtNPs-IgG). Finally, the concentration of DAS was negatively correlated with the pressure signal. In the presence of optimal conditions, matrix-matched calibration curves were plotted for wheat samples, in which an optimal IC50 value (half maximal inhibitory concentration) of 3.08 ng/g was achieved. The CTPDI was further applied to detect natural and blind wheat samples, and validation was carried out by liquid chromatography-tandem mass spectrometry. The results showed that CTPDI was highly appropriate and accurate for detection of DAS in wheat.

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