数字农科院2.0

Natural variation in the promoter of OsHMA3 contributes to differential grain cadmium accumulation between Indica and Japonica rice

文献类型： 外文期刊

作者： Chao-Lei Liu ; Zhen-Yu Gao +; Lian-Guang Shang +; Chang-Hong Yang ; Ban-Pu Ruan ; Da-Li Zeng ; Long-Biao Guo ; Fang-Jie Zhao ; Chao-Feng Huang *; Qian Qian *

作者机构：

期刊名称： Journal of Integrative Plant Biology

ISSN： 1672-9072

年卷期： 2020 年 62 卷 3 期

页码：

收录情况： JCR（2021版） ; CSCD（2020-2021年度） ; 科技核心（2020版）

摘要： Rice is a major source of cadmium (Cd) intake for Asian people. Indica rice usually accumulates more Cd in shoots and grains than Japonica rice. However, underlying genetic bases for differential Cd accumulation between Indica and Japonica rice are still unknown. In this study, we cloned a quantitative trait locus (QTL) grain Cd concentration on chromosome 7 (GCC7) responsible for differential grain Cd accumulation between two rice varieties by performing QTL analysis and map-based cloning. We found that the two GCC7 alleles, GCC7(PA64s) and GCC7(93-11), had different promoter activity of OsHMA3, leading to different OsHMA3 expression and different shoot and grain Cd concentrations. By analyzing the distribution of different haplotypes of GCC7 among diverse rice accessions, we discovered that the high and low Cd accumulation alleles, namely GCC7(93-11) and GCC7(PA64s), were preferentially distributed in Indica and Japonica rice, respectively. We further showed that the GCC7(PA64s) allele can be used to replace the GCC7(93-11) allele in the super cultivar 93-11 to reduce grain Cd concentration without adverse effect on agronomic traits. Our results thus reveal that the QTL GCC7 with sequence variation in the OsHMA3 promoter is an important determinant controlling differential grain Cd accumulation between Indica and Japonica rice.

分类号：

  • 相关文献
作者其他论文 更多>>