数字农科院2.0

A Low-Cost Novel Lateral Flow Nucleic Acid Assay (Lfnaa) For Yak Milk Authentication

文献类型： 外文期刊

作者： Wang, ZY; Li, TT; Yu, WJ; Qiao, L; Yang, SM; Chen, AL

作者机构：

关键词： Recombinant Polymerase Amplification (Rpa); Lateral Flow Nucleic Acid Assay (Lfnaa); Spacer C3; Ssdna Tail; Yak Milk Authentication

期刊名称： LWT-FOOD SCIENCE AND TECHNOLOGY

ISSN： 0023-6438

年卷期： 2020 年 122 卷

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： A low-cost novel lateral flow nucleic acid assay (LFNAA) was developed for rapid and sensitive authentication of yak milk on-site. In this method, a special abasic spacer C3 and tail nucleotide sequence modified reverse primers were innovatively designed for rapid isothermal recombinant polymerase amplification (RPA) to obtain a double-stranded amplicon with a single-stranded DNA tail, which could directly bind to gold nanoparticle (AuNP)-labeled detection probes, and produce a sandwich complex to show a red color on the test line of the strip. Compared to conventional RPA-lateral flow assay based on expensive antibodies, the proposed method in this work demonstrated massive cost reduction, avoided time-consuming and laborious AuNP-antibody conjugation process. The LFNAA revealed high specificity and sensitivity, which allowed detecting as low as 5% of yak milk within 40 min. This LFNAA facilitated non-specialized operators to field determine the authenticity of yak milk by naked eyes, and could be readily applied to other nucleic acid detection such as food safety inspection and point-of-care medical tests.

分类号：

  • 相关文献

[1]Rapid On-Site Detection Of Salmonella Pullorum Based On Lateral Flow Nucleic Acid Assay Combined With Recombinase Polymerase Amplification Reaction. Liu, R, Wang, ZY, Liu, XX, Chen, AL, Yang, SM. 2020

作者其他论文 更多>>