数字农科院2.0

Development And Evaluation Of A Chemiluminescence Immunoassay For Detecting Tropical Theileriosis

文献类型： 外文期刊

作者： Li, Z; Liu, JL; Ma, QY; Xu, JL; Wang, JM; Liu, AH; Li, YQ; Guan, GQ; Luo, JX; Yin, H

作者机构：

关键词： Theileria annulata; SVSP; CLIA; tropical theileriosis

期刊名称： ACTA TROPICA

ISSN： 0001-706X

年卷期： 2020 年 202 卷

页码：

收录情况： JCR（2021版）

摘要： Tropical theileriosis is a tick-borne lymphoproliferative disease of cattle caused by the apicomplexan parasite Theileria annulata, and leads to substantial economic losses to the livestock industry worldwide. Although various enzyme-linked immunosorbent assays (ELISAs) have been established to detect antibodies against T. annulata infection, a specific, rapid and reliable diagnostic assay is urgently needed for prevention and control of the disease. In the present study, a chemiluminescence immunoassay (CLIA) was developed based on the sub-telomeric variable secreted protein (SVSP) of T. annulata as a sero-diagnostic antigen. Following optimization of the CLIA working parameters, the working time of the method was less than 4.5 h. The sensitivity and specificity of the established CLIA was 98.8% and 97.5%, respectively, when the cut-off value of the percent positive (PP) was 26.1% for detecting serum samples (n = 242 T. annulata positive sera, n = 158 T. annulata negative sera). After comparing 180 serum samples from Gansu province, China, the concordance rate between the CLIA and a published rSpm2 ELISA method was 72.8%. In addition, 565 serum samples of cattle collected between 2017 and 2018 from four provinces in China were detected by the CLIA, and the seroprevalence for T. annulata ranged from 53.3% to 67.3% in these regions. Our findings demonstrated that the CLIA has high specificity, sensitivity and reliability, and could be used as a rapid detection assay for epidemiological investigations of T. annulata infection.

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