数字农科院2.0

Antiviral Activity Of Phage Display-Selected Peptides Against Japanese Encephalitis Virus Infection In Vitro And In Vivo

文献类型： 外文期刊

作者： Wei, JC; Hameed, M; Wang, X; Zhang, JJ; Guo, S; Anwar, MN; Pang, LL; Liu, K; Li, BB; Shao, DH; Qiu, YF; Zhong, DK; Zhou, B; Ma, ZY

作者机构：

关键词： Japanese encephalitis virus; Envelope protein; Phage display peptide library; Antiviral activity; Attachment

期刊名称： ANTIVIRAL RESEARCH

ISSN： 0166-3542

年卷期： 2020 年 174 卷

页码：

收录情况： JCR（2021版）

摘要： Japanese Encephalitis virus (JEV) is a zoonotic flavivirus that is the most significant etiological agent of childhood viral neurological infections. However, no specific antiviral drug is currently available to treat JEV infections. The JEV envelope (E) protein is a class II viral fusion protein that mediates host cell entry, making interference with the interaction between the E protein of JEV and its cognate receptors an attractive strategy for anti-JEV drug development. In this study, we identified a peptide derived from a phage display peptide library against the E protein of JEV, designated P1, that potentially inhibits in vitro and in vivo JEV infections. P1 inhibits JEV infection in BHK-21 cells with 50% inhibitory capacity at a concentration of 35.9 mu M. The time-of-addition assay indicates that JEV replication is significantly inhibited during pre-infection and co-infection of P1 with JEV while post-infection treatments with P1 have very little impact on JEV proliferation, showing that P1 inhibits JEV infection at early stages and indicating the potential prophylactic effect of P1. We adapted an in vitro BiFC assay system and demonstrated that P1 interacts with JEV E proteins and blocks their entry into cells. We also evaluated the therapeutic efficacy of P1 in a lethal JEV mouse model exhibiting systemic and brain infections. Interestingly, P1 treatment protected C57BL/6 mice against mortality, markedly reduced the viral loads in blood and brain, and diminished the histopathological lesions in the brain cells. In addition to controlling systemic infection, P1 has a very low level of cytotoxicity and acts in a sequence-specific manner, as scrambled peptide sP1 does not show any antiviral activity. In conclusion, our in vitro and in vivo experimental findings show that P1 possesses antiviral activity against JEV infections, is safe to use, and has potential for further development as an antiviral treatment against JEV infections.

分类号：

  • 相关文献

[1]Complex Virus-Host Interactions Involved In T.he Regulation Of Classical S wine Fever Virus Replication: A Minireview. Li, S, Wang, JH, Yang, Q, Anwar, MN, Yu, SX, Qiu, HJ. 2017

[2]A Single Mutation At Position 1.56 In The Envelope P rotein Of Tembusu Virus Is Responsible For Virus Tissue Tropism And Transmissibility In Ducks. Yan, DW, Shi, Y, Wang, HW, Li, GX, Li, XS, Wang, BB, Su, X, Wang, JH, Teng, QY, Yang, JM, Chen, HJ, Liu, QF, Ma, WJ, Li, ZJ. 2018

[3]Induction Of Avian Beta-Defensin 2 I.s Possibly Mediated By T he P38 Mapk Signal Pathway In Chicken Embryo Fibroblasts After Newcastle Disease Virus Infection. Liu, CG, Jiang, L, Liu, LL, Sun, L, Zhao, WJ, Chen, YQ, Qi, TM, Han, ZX, Shao, YH, Liu, SW, Ma, DY. 2018

[4]Interferon-Inducible Oligoadenylate Synthetase-Like Protein Acts A.s An Antiviral Effector A gainst Classical Swine Fever Virus Via The Mda5-Mediated Type I Interferon-Signaling Pathway. Li, LF, Yu, JH, Zhang, YX, Yang, Q, Li, YF, Zhang, LK, Wang, JH, Li, S, Luo, YZ, Sun, Y, Qiu, HJ. 2017

[5]A Novel Type I Interferon, I.nterferon Alphaomega, Shows Antiviral A ctivity Against Foot-And-Mouth Disease Virus In Vitro. Li, SF, Gong, MJ, Xie, YL, Shao, JJ, Zhao, FR, Zhang, YG, Chang, HY. 2019

[6]Antiviral Activity Of Merimepodib Against F.oot And Mouth Disease V irus In Vitro And In Vivo. Li, SF, Gong, MJ, Shao, JJ, Sun, YF, Zhang, YG, Chang, HY. 2019

[7]Doxycycline Enhances Adsorption And Inhibits E.arly-Stage Replication Of Porcine R eproductive And Respiratory Syndrome Virus In Vitro. Li, YM, Wu, ZC, Liu, K, Qi, PF, Xu, JP, Wei, JC, Li, BB, Shao, DH, Shi, YY, Qiu, YF, Ma, ZY. 2017

[8]Equine Myxovirus Resistance Protein 2 R.estricts Lentiviral Replication By B locking Nuclear Uptake Of Capsid Protein. Ji, S, Na, L, Ren, HL, Wang, YJ, Wang, XJ. 2018

[9]Antiviral Activity Of Porcine Interferon D.elta 8 Against Foot-And-Mouth D isease Virus In Vitro. Li, SF, Shao, JJ, Zhao, FR, Gong, MJ, Xie, YL, Chang, HY, Zhang, YG. 2018

[10]Toosendanin suppresses African swine fever virus replication through upregulating interferon regulatory factor 1 in porcine alveolar macrophage cultures. Liu Y., Zhang X., Liu Z., Huang L., Jia W., Lian X., Weng C., Zhang G., Qi W., Chen J.. 2022

[11]Antiviral Strategies Of Chinese Herbal Medicine Against Prrsv Infection. Bello-Onaghise, G, Wang, G, Han, X, Nsabimana, E, Cui, WQ, Yu, F, Zhang, YF, Wang, LG, Li, ZZ, Cai, XH, Li, YH. 2020

[12]Stabilized fermentation product of Cetobacterium somerae improves gut and liver health and antiviral immunity of zebrafish. Xie M., Xie Y., Li Y., Zhou W., Zhang Z., Yang Y., Olsen R.E., Ringø E., Ran C., Zhou Z.. 2022

[13]Proteasomal Degradation Of Nonstructural Protein 12 By Rnf114 Suppresses Porcine Reproductive And Respiratory Syndrome Virus Replication. Bai, YZ, Li, LW, Shan, TL, Zhang, YJ, Chen, XY, Gao, F, Jiang, YF, Zhou, YJ, Li, GX, Yu, LX, Kong, N, Ma, ZY, Tong, GZ. 2020

[14]Antiviral Effect Of Amiloride On R.eplication Of Foot And M outh Disease Virus In Cell Culture. Gong, MJ, Chang, YY, Shao, JJ, Li, SF, Zhang, YG, Chang, HY. 2019

[15]Restriction Of Porcine Reproductive And R.espiratory Syndrome Virus Replication B y Galectin-1. Li, LW, Zhao, K, Gao, F, Jiang, YF, Shan, TL, Tong, W, Zheng, H, Yu, LX, Li, GX, Ma, ZY, Tong, GZ. 2019

[16]Purification And Characterization Of A S.ecretory Alkaline Metalloprotease With H ighly Potent Antiviral Activity From Serratia Marcescens Strain S3. Qin, YX, Wang, J, Wang, FL, Shen, LL, Zhou, HX, Sun, HJ, Hao, KQ, Song, LY, Zhou, ZC, Zhang, CQ, Wu, YH, Yang, JG. 2019

[17]Antiviral Activity Of Brequinar Against F.oot-And-Mouth Disease Virus Infection I n Vitro And In Vivo. Li, SF, Gong, MJ, Sun, YF, Shao, JJ, Zhang, YG, Chang, HY. 2019

[18]Antiviral Effects Of Selected Impdh A.nd Dhodh Inhibitors Against F oot And Mouth Disease Virus. Gong, MJ, Li, SF, Chang, YY, Shao, JJ, Sun, YF, Ren, TT, Zhang, YG, Chang, HY. 2019

[19]A simple method for developing an infectious cDNA clone of Japanese encephalitis virus. Zheng, Hao,Zheng, Xuchen,Tong, Wu,Liu, Fei,Liang, Chao,Wang, Tao,Gao, Fei,Li, Liwei,Shan, Tongling,Li, Guoxin,Tong, Guangzhi,Zheng, Hao,Tong, Wu,Tong, Guangzhi.

[20]Effective inhibition of Japanese encephalitis virus replication by small interfering RNAs targeting the NS5 gene. Qi, Wen-Bao,Hua, Rong-Hong,Yan, Li-Ping,Tong, Guang-Zhi,Wu, Dong-Lai,Qi, Wen-Bao,Tong, Guang-Zhi,Zhang, Gui-Hong,Ren, Tao,Liao, Ming. 2008

作者其他论文 更多>>