数字农科院2.0

Tizoxanide Induces Autophagy By Inhibiting Pi3K/Akt/Mtor Pathway In Raw264.7 Macrophage Cells

文献类型: 外文期刊

作者: Shou, JQ; Wang, M; Cheng, XL; Wang, XY; Zhang, LF; Liu, YC; Fei, CZ; Wang, CM; Gu, F; Xue, FQ; Li, J; Zhang, KY

作者机构:

关键词: Autophagy; Tizoxanide; RAW264; 7 cells; PI3K; mTOR

期刊名称: ARCHIVES OF PHARMACAL RESEARCH

ISSN: 0253-6269

年卷期: 2020 年 43 卷 2 期

页码:

收录情况: JCR(2021版)

摘要: As the main metabolite of nitazoxanide, tizoxanide (TIZ) has a broad-spectrum anti-infective effect against parasites, bacteria, and virus. In this study, we investigated the effects of TIZ on autophagy by regulating the PI3K/Akt/mTOR signaling pathway. RAW264.7 macrophage cells were treated with various TIZ concentrations. Cell viability assay, transmission electron microscope, and immunofluorescence staining were used to detect the biological function of the macrophage cells, and the expression levels of the autophagy pathway-related proteins were measured by Western blot. Results revealed that TIZ promoted the conversion of LC3-I to LC3-II, the formation of autophagy vacuoles, and the degradation of SQSTM1/p62 in a concentration- and time-dependent manner in RAW264.7 cells. Treatment with TIZ increased the Beclin-1 expression level and inhibited PI3K, Akt, mTOR, and ULK1 activation. These effects were enhanced by pretreatment with rapamycin but attenuated by pretreatment with LY294002. In addition, the conversion of LC3-I to LC3-II was observed in Vero, 293T, and HepG2 cells treated with TIZ. These data suggested that TIZ may induce autophagy by inhibiting the Akt/mTOR/ULK1 signaling pathway in macrophages and other cells.

分类号:

  • 相关文献

[1]Tizoxanide Inhibits Inflammation In Lps-Activated R.aw264.7 Macrophages Via The S uppression Of Nf-Kappa B And Mapk Activation. Shou, JQ, Kong, XZ, Wang, XY, Tang, Y, Wang, CM, Wang, M, Zhang, LF, Liu, YC, Fei, CZ, Xue, FQ, Li, J, Zhang, KY. 2019

[2]Effect Of Melatonin On Casein E.xpression In Buffalo Mammary E pithelial Cells. Pang, Chun-Ying,Ma, Xiao-Ya,Lu, Xing-Rong,Liang, Xian-Wei,Liang, Sha-Sha,Duan, An-Qin,Deng, Ting-Xian,Riaz, Hasan,Riaz, Hasan,Yousuf, Muhammad Rizwan. 2018

[3]The Epigenetic Regulator Ezh2 Instructs Cd4 T Cell Response To Acute Viral Infection Via Coupling Of Cell Expansion And Metabolic Fitness. Li, R, Pan, ZW, Wu, JL, Yue, S, Lin, Y, Yang, Y, Li, ZR, Hu, L, Tang, JF, Shan, LL, Tian, Q, Jiang, P, Wei, P, Ye, LL, Liu, PH, Chen, XY. 2020

[4]Transcriptomic Analysis Following Artificial Selection for Grasshopper Size. Liu, Shao-Fang,Liu, Shao-Fang,Tu, Xiong-Bing,Li, Shuang,Chen, Jun,Cui, Dong-Nan,Whitman, Douglas W.,Zhang, Ze-Hua,Ullah, Hidayat,Ullah, Hidayat. 2020

[5]Fungal Immunomodulatory Protein From Nectria H.aematococca Suppresses Growth Of H uman Lung Adenocarcinoma By Inhibiting The Pi3K/Akt Pathway. Xie, YY, Li, SY, Sun, L, Liu, SJ, Wang, FZ, Wen, BT, Sun, LC, Fang, XD, Chai, YS, Cao, H, Jia, N, Gu, TY, Lou, XM, Xin, FJ. 2018

[6]Antagonism between PTP1B and PTK Mediates Adults’ Insulin-Like Signaling Regulation of Egg Diapause in the Migratory Locust. Shuang Li,Jie Wang,Dongnan Cui,Kun Hao,Jun Chen,Xiongbing Tu,Zehua Zhang. 2021

[7]Antagonism between PTP1B and PTK Mediates Adults’ Insulin-Like Signaling Regulation of Egg Diapause in the Migratory Locust. Shuang Li,Jie Wang,Dongnan Cui,Kun Hao,Jun Chen,Xiongbing Tu,Zehua Zhang. 2021

[8]Squid Ink Polysaccharide Prevents Autophagy A.nd Oxidative Stress Affected B y Cyclophosphamide In Leydig Cells Of Mice: A Pilot Study. Gu, Yi-Peng,Yang, Xiao-Mei,Duan, Zhen-Hua,Gu, Yi-Peng,Luo, Ping,Zhang, Da-Yan,Gu, Yi-Peng,Shang, Jiang-Hua,Liu, Hua-Zhong,Xiao, Wei. 2017

[9]Loss of Ssql leads to mitochondrial dysfunction, activation of autophagy and cell cycle arrest due to iron overload triggered by mitochondrial iron-sulfur cluster assembly defects in Candida albicans. Zhang, Dan,Yu, Qilin,Zhao, Qiang,Xiao, Chenpeng,Zhang, Kai,Jia, Chang,Chen, Sijia,Zhang, Bing,Li, Mingchun,Zhang, Biao,Dong, Yijie,Li, Mingchun.

[10]Autophagy induced by infectious hematopoietic necrosis virus inhibits intracellular viral replication and extracellular viral yields in epithelioma papulosum cyprini cell line. Zhao, Jing-Zhuang,Xu, Li-Ming,Liu, Miao,Yin, Jia-Sheng,Liu, Hong-Bai,Lu, Tong-Yan,Zhang, Zhen-yu.

[11]Autophagy regulates spermatid differentiation via degradation of PDLIM1. Shang, Yongliang,Wang, Hongna,Zhao, Haichao,Liu, Chao,Liu, Weixiao,Song, Zhenhua,Xu, Zhiliang,Li, Wei,Shang, Yongliang,Wang, Hongna,Zhao, Haichao,Liu, Chao,Song, Zhenhua,Xu, Zhiliang,Jia, Pengfei,Yang, Lin,Jia, Pengfei,Yang, Lin,Wang, Yanfang.

[12]Contribution of VMA5 to vacuolar function, stress response, ion homeostasis and autophagy in Candida albicans. Jia, Chang,Yu, Qilin,Xiao, Chenpeng,Dong, Yijie,Zhang, Meng,Zhang, Dan,Li, Mingchun,Dong, Yijie,Zhao, Qiang,Zhang, Biao.

[13]Thapsigargin induces apoptosis when autophagy is inhibited in HepG2 cells and both processes are regulated by ROS-dependent pathway. Wang, Congcong,Tang, Shusheng,Zhao, Dongxu,Zhang, Chaoming,Zhang, Shen,Deng, Sijun,Zhou, Yan,Xiao, Xilong,Wang, Congcong,Li, Tao.

[14]Silencing of BAG3 promotes the sensitivity of ovarian cancer cells to cisplatin via inhibition of autophagy. Qiu, Shuang,Jin, Ye,An, Qi,Zheng, Jianhua,Sun, Liang,Weng, Changjiang.

[15]Infectious Bursal Disease Virus Subverts Autophagic Vacuoles To Promote Viral Maturation and Release. Wang, Yongqiang,Duan, Yulu,Han, Chunyan,Yao, Shuai,Qi, Xiaole,Gao, Yulong,Zhang, Lizhou,Gao, Li,Gao, Honglei,Shen, Nan,Wang, Jingfei,Wang, Xiaomei,Maier, Helena J.,Britton, Paul,Chen, Lei.

[16]Targeting autophagy to enhance oncolytic virus-based cancer therapy. Meng, Songshu,Xu, Jiansheng,Wu, Yantao,Ding, Chan.

[17]Endoplasmic reticulum stress-mediated autophagy contributes to bluetongue virus infection via the PERK-eIF2 alpha pathway. Lv, Shuang,Sun, En-Cheng,Xu, Qing-Yuan,Zhang, Ji-Kai,Wu, Dong-Lai.

[18]Antifungal activities and effect of Lactobacillus casei AST18 on the mycelia morphology and ultrastructure of Penicillium chrysogenum. Li, Hongjuan,Zhang, Shuwen,Lu, Jing,Liu, Lu,Uluko, Hankie,Pang, Xiaoyang,Xue, Haixiao,Zhao, Lili,Kong, Fanhua,Lv, Jiaping,Sun, Yanjun. 2014

[19]Autophagy activated by duck enteritis virus infection positively affects its replication. Yin, Hai-Chang,Zhao, Li-Li,Niu, Yin-Jie,Lu, Tao-Feng,Han, Ling-Xia,Liu, Sheng-Wang,Chen, Hong-Yan,Li, Si-Qi,Jiang, Xin-Jie,Xu, Li-Jing.

[20]Role of autophagy in cellular response to infection with Orf virus Jilin isolate. Lan, Yungang,Song, Deguang,He, Wenqi,Zhang, Di,Huang, Houshuang,Bi, Jingying,Gao, Feng,Zhao, Kui,Wang, Gaili,Gao, Feng.

作者其他论文 更多>>