数字农科院2.0

Fluorescent Immunochromatographic Assay For Quantitative Detection Of The Foot-And-Mouth Disease Virus Serotype O Antibody

文献类型： 外文期刊

作者： Hou, FP; Bai, MY; Zhang, Y; Liu, HY; Sun, SQ; Guo, HC

作者机构：

关键词： Foot-and-mouth disease; Point-of-care test; Virus-like-particles; High sensitivity; Quantitative detection

期刊名称： MICROCHEMICAL JOURNAL

ISSN： 0026-265X

年卷期： 2020 年 155 卷

页码：

收录情况： JCR（2021版）

摘要： Ultrasensitive and quantitative real-time detection of infectious disease has long been a challenge in prevention and diagnosis of epidemic disease. It's essential to develop a quantitative point-of-care (POC) diagnostic approach. Herein, a fluorescent platform was designed, which was a sandwich type and applied noninfectious virus-like-particles (VLPs) as capture antigen, making the process safer and more efficient. The fluorescent europium microparticles (EuMSs) conjugated with staphylococcal protein-A (SPA), then antibody was bind with SPA and target antibody was further captured by VLPs at test line. The excessive labelled-SPA was captured by rabbit IgG at control line. Specifically, the fluorescent signal intensity at test line and control line was relative to the concentration of the target antibody. Moreover, the fluorescent assay can not only be monitored through fluorescent signal output within 15 min but also be observed under UV light with 5 min. The limit of detection (LOD) for quantitative detection of serotype O antibody against foot-and-mouth disease virus (O-FMDV) in serum was over 60 times more sensitive than conventional colored gold-colloids. Owing to the advantages of sensitivity and specificity, the fluorescent immunochromatographic test strips (ICTSs) would be a potential tool for rapid and sensitive probe for quantitative determination of O-FMDV antibody.

分类号：

  • 相关文献

[1]AIEgens enabled ultrasensitive point-of-care test for multiple targets of food safety: Aflatoxin B1 and cyclopiazonic acid as an example. Xiaofeng Hu,Pengfei Zhang,Du Wang,Jun Jiang,Xiaomei Chen,Yong Liu,Zhaowei Zhang,Ben Zhong Tang,Peiwu Li. 2021

[2]A High-Sensitivity Thermal Analysis Immunochromatographic S.ensor Based On Au N anoparticle-Enhanced Two-Dimensional Black Phosphorus Photothermal-Sensing Materials. Wang, Junying,Wang, Shuo,Sheng, Wei,Li, Shijie,Wang, Junping,Wen, Wenjun,Zhang, Ying. 2019

[3]Lanthanide-Labeled Fluorescent-Nanoparticle Immunochromatographic Strips Enable Rapid And Quantitative Detection Of Escherichia Coli O157:H7 In Food Samples. Wang, Q, Long, MY, Lv, CY, Xin, SP, Han, XG, Jiang, W. 2020

[4]Fluorometric Determination Of Rnase H V.ia A Dnazyme Conjugated T o Reduced Graphene Oxide, And Its Application To Screening Forinhibitors And Activators. Tong, CY, Zhou, T, Zhao, C, Yuan, LQ, Xu, Y, Liu, B, Fan, JL, Li, D, Zhu, AG. 2019

[5]Toward agricultural ammonia volatilization monitoring: A flexible polyaniline/Ti3C2Tx hybrid sensitive films based gas sensor. 李娴,Jianlong Xu,Yadong Jiang,Zaizhou He,Bohao Liu,Haikuan Xie,Hu Li,李哲敏,Yang Wang,Huiling Tai. 2020

[6]High Sensitivity Immunoassays For Small M.olecule Compounds Detection - N ovel Noncompetitive Immunoassay Designs. Li, YS, Zhang, GZ, Mao, X, Yang, SP, De Ruyck, K, Wu, YN. 2018

[7]Fe-N-C single-atom nanozyme for ultrasensitive, on-site and multiplex detection of mycotoxins using lateral flow immunoassay. Cai Xinfa, Ma Fei, Jiang Jun, Yang Xianglong, Zhang Zhaowei, Jian Zelang, Liang Meijuan, Li Peiwu, Yu Li. 2022

[8]Establishment of a Murine alpha nu beta 1 Transgenic CHO-K1 Cell Line and its Susceptibility to Foot-and-Mouth Disease Virus Type Asia 1/HN/2006 in China. Zhang, Y.,Zheng, H. X.,Zhang, Z. D.,Jin, Y.,Yang, F.,He, J. J.,Cao, W. J.,Sun, D. H.,Lv, L.. 2013

[9]Recombinant Bivalent Vaccine against Foot-and-Mouth Disease Virus Serotype O/A Infection in Guinea Pig. Yi, JZ,Liu, MQ,Zhu, CZ,Zhang, Q,Sheng, ZT,Du, QY,Yan, WY,Zheng, ZX.

[10]Genetic characterization of a new pandemic Southeast Asia topotype strain of serotype O foot-and-mouth disease virus isolated in China during 2010. Zheng, Haixue,He, Jijun,Guo, Jianhong,Jin, Ye,Yang, Fan,Lv, Lv,Liu, Xiangtao. 2012

[11]Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs. Cao, Yimei,Lu, Zengjun,Sun, Jiachuan,Bai, Xingwen,Sun, Pu,Bao, Huifang,Chen, Yingli,Guo, Jianhong,Li, Dong,Liu, Xiangtao,Liu, Zaixin.

[12]An overview of control strategy and diagnostic technology for foot-and-mouth disease in China. Ding, Yao-Zhong,Chen, Hao-Tai,Zhang, Jie,Zhou, Jian-Hua,Ma, Li-Na,Zhang, Liang,Gu, Yuanxin,Liu, Yong-Sheng. 2013

[13]A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C. Ding, Yao-zhong,Zhou, Jian-hua,Ma, Li-na,Qi, Yan-ni,Zhang, Jie,Zhang, Yong-guang,Ding, Yao-zhong,Zhang, Jie,Zhang, Yong-guang,Wei, Gang.

[14]Rapid typing of foot-and-mouth disease serotype Asia 1 by reverse transcription loop-mediated isothermal amplification. Liu, Yong-sheng,Liu, Xiang-tao. 2011

[15]Induction of Partial Protection against Foot and Mouth Disease Virus in Guinea Pigs by Neutralization with the Integrin beta 6-1 Subunit. Zhang, Yan,Sun, Yingjun,Yang, Fan,Guo, Jianhong,He, Jijun,Wu, Qiong,Cao, Weijun,Lv, Lv,Zheng, Haixue,Zhang, Zhidong. 2013

[16]Hollow Mesoporous Silica Nanoparticles As D.elivery Vehicle Of Foot-And-Mouth D isease Virus-Like Particles Induce Persistent Immune Responses In Guinea Pigs. Bai, MY,Dong, H,Su, X,Jin, Y,Sun, SQ,Zhang, YP,Yang, YS,Guo, HC. 2019

[17]Development And Validation Of A C.ompetitive Elisa Based On B acterium-Original Virus-Like Particles Of Serotype O Foot-And-Mouth Disease Virus For Detecting Serum Antibodies. Ran, XH, Yang, ZY, Bai, MY, Zhang, Y, Wen, XB, Guo, HC, Sun, SQ. 2019

[18]Promotion Of Cellular And Humoral Immunity Against Foot-And-Mouth Disease Virus By Immunization With Virus-Like Particles Encapsulated In Monophosphoryl Lipid A And Liposomes. Kim, WS, Zhi, Y, Guo, HC, Byun, EB, Lim, JH, Seo, HS. 2020

[19]Article four simple biomimetic mineralization methods to improve the thermostability and immunogenicity of virus-like particles as a vaccine against foot-and-mouth disease. Mengnan Guo,Jiajun Li,Zhidong Teng,Mei Ren,Hu Dong,Yun Zhang,Jiaxi Ru,Ping Du,Shiqi Sun,Huichen Guo. 2021

[20]Selection of vaccine candidate for foot-and-mouth disease virus serotype o using a blocking enzyme-linked immunosorbent assay. Cao Yimei,Li Kun,Xing Xiangchuan,Bao Huifang,Huang Nana,Zhu Guoqiang,Bai Xingwen,Sun Pu,Fu Yuanfang,Li Pinghua,Zhang Jing,Ma Xueqing,Li Dong,Liu Zaixin,Lu Zengjun. 2021

作者其他论文 更多>>