数字农科院2.0

Characterization Of Maltobp1, A Minus-C Odorant-Binding Protein, From The Japanese Pine Sawyer Beetle, Monochamus Alternatus Hope (Coleoptera: Cerambycidae)

文献类型： 外文期刊

作者： Zhang, FM; Merchant, A; Zhao, ZB; Zhang, YH; Zhang, J; Zhang, QW; Wang, QH; Zhou, XG; Li, XR

作者机构：

关键词： Monochamus alternatus; olfactory; odorant binding protein; fluorescence binding assay; plant volatile

期刊名称： FRONTIERS IN PHYSIOLOGY

ISSN： 1664-042X

年卷期： 2020 年 11 卷

页码：

收录情况： JCR（2021版）

摘要： Insect Odorant-Binding Proteins (OBPs) play crucial roles in the discrimination, binding and transportation of odorants. Herein, the full-length cDNA sequence of Minus-C OBP1 (MaltOBP1) from the Japanese pine sawyer beetle, Monochamus alternatus, was cloned by 3 ' and 5 ' RACE-PCR and analyzed. The results showed that MaltOBP1 contains a 435 bp open reading frame (ORF) that encodes 144 amino acids, including a 21-amino acid signal peptide at the N-terminus. The matured MaltOBP1 protein possesses a predicted molecular weight of about 14 kDa and consists of six alpha-helices, creating an open binding pocket, and two disulfide bridges. Immunoblotting results showed that MaltOBP1 was most highly expressed in antennae in both sexes, followed by wings and legs. Fluorescence assays demonstrated that MaltOBP1 protein exhibited high binding affinity with (R)-(+)-alpha-pinene, (-)-beta-pinene, trans-caryophyllene, (R)-(+)-limonene and (-)-verbenone, which are the main volatile compounds of the pine tree. Our combined results suggest that MaltOBP1 plays a role in host seeking behavior in M. alternatus.

分类号：

  • 相关文献

[1]Coordinative mediation of the response to alarm pheromones by three odorant binding proteins in the green peach aphid Myzus persicae. Qian Wang,Jing-Tao Liu,Yong-Jun Zhang,Ju-Lian Chen,Xian-Chun Li,Pei Liang,Xi-Wu Gao,Jing-Jiang Zhou. 2021

[2]The construction and evaluation of secretory expression engineering bacteria for the trans-Cry3Aa-T-HasA fusion protein against the Monochamus alternatus vector. Han, Xiaohong,Huang, Chenyan,Qi, Huan,Zhu, Yukun,Hu, Xinran,Wen, Yingxin,Long, Yirong,Xu, Lei,Zhang, Feiping. 2024

[3]Functional Studies Of Sex Pheromone R.eceptors In Asian Corn B orer Ostrinia Furnacalis. Liu, Wei,Jiang, Xing-chuan,Cao, Song,Wang, Gui-rong,Yang, Bin. 2018

[4]Molecular Evidence For Adaptive Evolution Of Olfactory-Related Genes In Cervids. Ba, HX, Qin, T, Cai, ZX, Liu, WY, Li, CY. 2020

[5]Identification And Characterization Of Odorant B.inding Proteins In The F orelegs Of Adelphocoris Lineolatus (Goeze). Sun, L, Wang, Q, Wang, Q, Dong, K, Xiao, Y, Zhang, YJ. 2017

[6]Integrative Transcriptomic And Genomic Analysis O.f Odorant Binding Proteins A nd Chemosensory Proteins In Aphids. Li, X. -C.,Gu, S. -H.,Chen, J. -L.,Liu, J. -T.,Liu, J. -T.,Li, X. -C.,Wang, Q.,Zhou, J. -J.,Xu, W. -Y.,Xu, W. -Y.,Zhang, Q.,Li, X. -C.,Huang, G. -Z.,Zhang, Y. -J.. 2019

[7]Variation In Both Host Defense And Prior Herbivory Can Alter Plant-Vector-Virus Interactions. Shi, XB, Preisser, EL, Liu, BM, Pan, HP, Xiang, M, Xie, W, Wang, SL, Wu, QJ, Li, CY, Liu, Y, Zhou, XG, Zhang, YJ. 2019

[8]Bt Rice Plants May Protect N.eighbouring Non-Bt Rice Plants A gainst The Striped Stem Borer, Chilo Suppressalis. Hu, Xiaoyun,Jiao, Yaoyu,Li, Yunhe,Romeis, Jorg,Romeis, Jorg,Peng, Yufa,Wu, Kongming. 2018

[9]Does Background Odor In Tea G.ardens Mask Attractants? Screening A nd Application Of Attractants For Empoasca Onukii Matsuda. Xu, XX, Cai, XM, Bian, L, Luo, ZX, Li, ZQ, Chen, ZM. 2017

[10]Volatiles From Sophora Japonica Flowers A.ttract Harmonia Axyridis Adults ( Coleoptera: Coccinellidae). Xu Bin,Zhang Wei,Lu Yan-hui,Yang Yi-zhong,Xiu Chun-li,Xu Bin,Pan Hong-sheng,Zhang Wei,Xu Bin. 2019

[11]Plants Pre-Infested With Viruliferous Med/Q C.ryptic Species Promotes Subsequent B emisia Tabaci Infestation. Shi, XB, Chen, G, Pan, HP, Xie, W, Wu, QJ, Wang, SL, Liu, Y, Zhou, XG, Zhang, YJ. 2018

[12]Detecting Host-Plant Volatiles With Odorant Receptors From Grapholita Molesta (Busck) (Lepidoptera: Tortricidae). Chen, LH, Tian, K, Xu, XL, Fang, AS, Cheng, WN, Wang, GR, Liu, W, Wu, JX. 2020

[13]Molecular Characterization And Ligand-Binding Properties O.f Six Odorant-Binding Proteins ( Obps) From Aphis Gossypii. Cui, Jin-Jie,Luo, Jun-Yu,Wang, Chun-Yi,Gao, Xue-Ke,Lu, Li -Min,Zhang, Shuai,Zhu, Xiang-Zhen,Wang, Li,Zhang, Li-Juan. 2018

[14]Identification Of Odorant-Binding Proteins And Functional Analysis Of Antenna-Specific Aplaobp1 In The Emerald Ash Borer, Agrilus Planipennis. Zhang, Yong-Jun,Shan, Shuang,Khashaveh, Adel,Wang, Shan-Ning,Shan, Shuang,Zhang, Fan,Wang, He,Yu, Guo-Yue,Dhiloo, Khalid Hussain. 2020

[15]Three Odorant Binding Proteins May R.egulate The Behavioural Response O f Chrysopa Pallens To Plant Volatiles And The Aphid Alarm Pheromone (E)-Beta-Farnesene. Li, ZQ, Zhang, S, Cai, XM, Luo, JY, Dong, SL, Cui, JJ, Chen, ZM. 2017

[16]The Mechanism Underlying Obp Heterodimer Formation And The Recognition Of Odors In Holotrichia Oblita Faldermann. Wei, HS, Duan, HX, Li, KB, Zhang, S, Wei, ZJ, Yin, J. 2020

[17]Mouthparts Enriched Odorant Binding Protein A.fasobp11 Plays A Role I n The Gustatory Perception Of Adelphocoris Fasciaticollis. Li, ZB, Wei, Y, Sun, LG, An, XK, Dhiloo, KH, Wang, Q, Xiao, Y, Khashaveh, A, Gu, SH, Zhang, YJ. 2019

作者其他论文 更多>>