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A tripartite ssDNA mycovirus from a plant pathogenic fungus is infectious as cloned DNA and purified virions

文献类型： 外文期刊

作者： Li, Pengfei;Guo, Lihua;Qiu, Dewen;Zhou, Xueping;Wang, Shuangchao;Zhang, Lihang

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期刊名称： Science Advances

ISSN： 2375-2548

年卷期： 2020 年 6 卷 14 期

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收录情况： JCR（2021版） ; EI（2021版）

摘要： Here, we describe a tripartite circular single-stranded (ss) DNA mycovirus, named Fusarium graminearum gemytripvirus 1 (FgGMTV1). The genome of FgGMTV1 comprises three circular ssDNA segments (DNA-A, DNA-B, and DNA-C). Sequence alignments and phylogenetic analyses showed that FgGMTV1 is nested within the family Genomoviridae. We also constructed the first infectious DNA clones of a DNA mycovirus. Our results show that DNA-A and DNA-B are mutually interdependent for their replication and are associated with severely reduced colony growth and hypovirulence. DNA-C relies on DNA-A and DNA-B for replication and is necessary for the recovery of abnormal fungal phenotypes. DNA-C also enhances the accumulation of viral DNA in infected fungi and permits stable colonization and easy transmission via conidia. This is the first multipartite DNA virus isolated from a fungus. Our phylogenetic analyses also suggest that the multipartite genome of FgGMTV1 may have evolved from a monopartite genome of an ancient genomovirus.

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