数字农科院2.0

Recombinant Infectious Bronchitis Coronavirus H120 With The Spike Protein S1 Gene Of The Nephropathogenic Ibyz Strain Remains Attenuated But Induces Protective Immunity

文献类型： 外文期刊

作者： Gao, Mingyan;Yu, Yan;Zhao, Xiumei;Cheng, Xu;Zhou, Sheng;Jiang, Yi

作者机构：

关键词： Infectious bronchitis virus; Attenuation; Spike; Vaccine; QX-like

期刊名称： VACCINE

ISSN： 0264-410X

年卷期： 2020 年 38 卷 15 期

页码：

摘要： Infectious bronchitis (IB) is a highly infectious viral disease responsible for major economic losses in the poultry industry. A reverse genetic vaccine is a safe, rapid, and effective method of achieving IB prevention and control. In this study, we constructed the recombinant strain, rH120-S1/YZ, using a reverse genetic system, based on the backbone of the H120 vaccine strain, with the S1 gene replaced with that of the QX-like nephropathogenic strain, ck/CH/IBYZ/2011, isolated in China. The results of dwarf chicken embryos, growth kinetics, and viral titration in the embryos demonstrated that the biological characteristics of the recombinant virus remained unchanged. Like the rH120-infected group and in contrast to the rIBYZ-infected group, no mortality, clinical signs, or lesions were observed in the lungs or kidneys of young chickens inoculated with rH120-S1/YZ. The viral loads in various tissues, cloacal, and oral swabs was lower in most types of samples, indicating that the rH120-S1/YZ strain was highly safe in chicks. Compared to rH120 vaccination group, when the efficacy of this strain was evaluated against the QX-like IBV strain, better protection, with 100% survival rate and no disease symptom or gross lesion was observed in the chickens vaccinated with rH120-S1/YZ. Increased levels of IBV-specific antibodies were detected in the serum of the rH120-S1/YZ-vaccinated animals 14 days post-vaccination. Collectively, our results suggest that the recombinant strain, rH120-S1/YZ, may represent a promising vaccine candidate against QX-like IBVs. (C) 2020 Elsevier Ltd. All rights reserved.

分类号：

  • 相关文献

[1]Phenotypic And Genotypic Comparison Of A Live-Attenuated Genotype I Japanese Encephalitis Virus Sd12-F120 Strain With Its Virulent Parental Sd12 Strain. Anwar, MN, Wang, X, Hameed, M, Wahaab, A, Li, CX, Sharma, M, Pang, LL, Malik, MI, Liu, K, Li, BB, Qiu, YF, Wei, JC, Ma, ZY. 2020

[2]Adaptation of a live-attenuated genotype I Japanese encephalitis virus to vero cells is associated with mutations in structural protein genes. Muhammad Naveed Anwar,Shuang Guo,Wang Xin,Muddassar Hameed,Abdul Wahaab,Xiaochun Ma,Aman Ullah Khan,Sajid Ur Rahman,Donghua Shao,Zongjie Li,Ke Liu,Beibei Li,Yafeng Qiu,Zhiyong Ma,Jianchao Wei. 2021

[3]Attenuation Of Equine Lentivirus Alters M.itochondrial Protein Expression Profile F rom Inflammation To Apoptosis. Du, C, Duan, YY, Wang, XF, Lin, YZ, Na, L, Wang, XH, Chen, KW, Wang, XJ. 2019

[4]Genome-Wide Identification And Expression Profiling O.f The Tcp Family G enes In Spike And Grain Development Of Wheat (Triticum Aestivum L.). Zhao, JM, Zhai, ZW, Li, YA, Geng, SF, Song, GY, Guan, JT, Jia, ML, Wang, FL, Sun, GL, Feng, N, Kong, XC, Chen, L, Mao, L, Li, AL. 2018

[5]Molecular pharming in maize endosperm: A proof of concept with recombinant SARS-CoV-2 antigen production. Yuxin Tai,,Shujun Meng,,Yiteng Zhang,,Jianfeng Weng,,Xinhai Li,,Qingyu Wu. 2025

[6]Attenuation Of An Original Us P.orcine Epidemic Diarrhea Virus S train Pc22A Via Serial Cell Culture Passage. Lin, CM, Hou, Y, Marthaler, DG, Gao, X, Liu, X, Zheng, L, Saif, LJ, Wang, QH. 2017

[7]Comparative Genomic Analyses Of A V.irulent Pseudorabies Virus And A Series Of Its In Vitro Passaged Strains. Ye, C, Wu, JQ, Tong, W, Shan, TL, Cheng, XF, Xu, JJ, Liang, C, Zheng, H, Li, GX, Tong, GZ. 2018

[8]Isolation, Identification And Attenuation Of A. Pathogenic Duck Hepatitis V irus Type 1 In China, And Complete Genomic Sequence Comparison Between The Embryo-Passaged, Attenuated Derivatives And Their Parent Strain. Liu, X, Kong, X. 2019

[9]Emergence of novel nephropathogenic infectious bronchitis viruses currently circulating in Chinese chicken flocks. Xu, Qianqian,Han, Zongxi,Wang, Qiuling,Zhang, Tingting,Gao, Mengying,Zhao, Yan,Shao, Yuhao,Li, Huixin,Kong, Xiangang,Liu, Shengwang.

[10]Infectious bronchitis virus nucleoprotein specific CTL response is generated prior to serum IgG. Liu, Guangliang,Wang, Qun,Liu, Nihong,Xiao, Yihong,Tong, Tiegang,Liu, Shengwang,Wu, Donglai. 2012

[11]Co-infection of H9N2 subtype avian influenza virus and infectious bronchitis virus decreases SP-A expression level in chickens. Huang, Qi,Wang, Kai,Pan, Ling,Qi, Kezong,Liu, Hongmei,Huang, Qi,Wang, Kai,Chen, Hongjun.

[12]Development and characterization of a recombinant infectious bronchitis virus expressing the ectodomain region of S1 gene of H120 strain. Wei, Yan-Quan,Guo, Hui-Chen,Dong, Hu,Wang, Hai-Ming,Xu, Jin,Sun, De-Hui,Cai, Xue-Peng,Liu, Ding-Xiang,Sun, Shi-Qi,Liu, Ding-Xiang,Fang, Shou-Guo.

[13]Prediction and identification of novel IBV S1 protein derived CTL epitopes in chicken. Tan, Lei,Liao, Ying,Fan, Jin,Zhang, Yuqiang,Mao, Xiang,Sun, Yingjie,Song, Cuiping,Qiu, Xusheng,Meng, Chunchun,Ding, Chan,Ding, Chan.

[14]Infectious bronchitis virus poly-epitope-based vaccine protects chickens from acute infection. Tan, Lei,Zhang, Yuqiang,Liu, Fang,Yuan, Yanmei,Zhan, Yuan,Sun, Yingjie,Qiu, Xusheng,Meng, Chunchun,Song, Cuiping,Ding, Chan,Ding, Chan.

[15]Protection of chickens against infectious bronchitis by a recombinant fowlpox virus co-expressing IBV-S1 and chicken IFN gamma. Wang, Yun-Feng,Sun, Yong-Ke,Tian, Zhan-Cheng,Shi, Xing-Ming,Tong, Guang-Zhi,Liu, Sheng-Wang,Zhi, Hai-Dong,Kong, Xian-Gang,Wang, Mei. 2009

[16]Transcriptome analysis of chicken kidney tissues following coronavirus avian infectious bronchitis virus infection. Cong, Feng,Liu, Xiaoli,Han, Zongxi,Shao, Yuhao,Kong, Xiangang,Liu, Shengwang. 2013

[17]Recombinant duck enteritis viruses expressing major structural proteins of the infectious bronchitis virus provide protection against infectious bronchitis in chickens. Li, Huixin,Wang, Yulong,Han, Zongxi,Wang, Yu,Liang, Shulin,Jiang, Lu,Kong, Xiangang,Liu, Shengwang,Wang, Yulong,Hu, Yonghao.

[18]Evaluation of recombinant fowlpox virus expressing infectious bronchitis virus S1 gene and chicken interferon-gamma gene for immune protection against heterologous strains. Shi, Xing-Ming,Zhao, Yan,Gao, Hong-Bo,Jing, Zhang,Wang, Mei,Cui, Hong-Yu,Tong, Gang-Zhi,Wang, Yun-Feng. 2011

[19]Recombinant Newcastle disease virus expressing the infectious bronchitis virus S1 gene protects chickens against Newcastle disease virus and infectious bronchitis virus challenge. Zhao, Ran,Sun, Junfeng,Qi, Tianming,Zhao, Wen,Han, Zongxi,Liu, Shengwang,Zhao, Ran,Yang, Xiaopu,Liu, Shengwang.

[20]Differential modulation of avian beta-defensin and Toll-like receptor expression in chickens infected with infectious bronchitis virus. Xu, Yang,Zhang, Tingting,Xu, Qianqian,Liang, Shuling,Ma, Deying,Han, Zongxi,Shao, Yuhao,Liu, Shengwang.

作者其他论文 更多>>