数字农科院2.0

Carboxymethylation Of Qingke Beta-Glucans And Their Physicochemical Properties And Biological Activities

文献类型： 外文期刊

作者： Guo, H; Feng, KL; Zhou, J; Liu, L; Wei, SY; Zhao, L; Qin, W; Gan, RY; Wu, DT

作者机构：

关键词： Hordeum vulgare L.; Carboxymethylated modification; Structural characteristics; Antioxidant activity; Hypolipidemic activity

期刊名称： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

ISSN： 0141-8130

年卷期： 2020 年 147 卷

页码：

收录情况： JCR（2021版）

摘要： In this study, the response surface methodology was used to optimize the reaction conditions of carboxymethylated modification of Qingke beta-glucans (QG), and effects of different degrees of carboxymethylation (high, medium, and low) on their structural characteristics, in vitro antioxidant activities, and in vitro hypolipidemic activities were studied. The optimal reaction conditions of carboxymethylated Qingke beta-glucans (QG-Cs) with high degree of carboxymethylation (DC = 0.90) were as follows: reaction temperature of 62.1 degrees C, reaction time of 329 h, and concentration of chloroacetic add of 1.75 mol/L. Results demonstrated that the carboxymethylated modification significantly affected the solubilities, molar ratios of constituent monosaccharides, molecular weights, and apparent viscosities of QG. Indeed, the QG-Cs exhibited much higher antioxidant activities (reducing powers, NO, and DPPH radical scavenging activities), in vitro binding properties (fat, bile acid, and cholesterol binding capacities), and pancreatic lipase inhibition activities than that of QG. Furthermore, results showed that bioactivities of QG-Cs were closely correlated to their carboxymethyl groups. Results suggested that the carboxymethylated modification could be an efficient method for enhancing bioactivities of QG, and QG-Cs had good potential applications in bio-pharmaceutical industry. (C) 2020 Elsevier B.V. All rights reserved.

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