数字农科院2.0

Differential Abilities of Mammalian Cathelicidins to Inhibit Bacterial Biofilm Formation and Promote Multifaceted Immune Functions of Neutrophils

文献类型: 外文期刊

作者: Zan, Yanan;Zhang, Huihui;Jin, Mingjie;Xie, Fang;Jin, Mingjie;Liu, Siguo;Zhang, Xinyuan;Zhang, Wanjiang;Zhang, Xinyuan;Zhang, Yueling

作者机构:

关键词: antimicrobial peptide; cathelicidins; bactericidal activity; antibiofilm; immunomodulation; neutrophils; chemotaxis; extracellular traps; Pseudomonas aeruginosa

期刊名称: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN: 1661-6596

年卷期: 2020 年 21 卷 5 期

页码:

收录情况: JCR(2021版)

摘要: Mammalian cathelicidins act as the potent microbicidal molecules for controlling bacterial infection, and are considered promising alternatives to traditional antibiotics. Their ability to modulate host immune responses, as well as their bactericidal activities, is essential for therapeutic interventions. In this study, we compared the bactericidal activities, antibiofilm activities and immune-modulatory properties of cathelicidins BMAP-27, BMAP-34, mCRAMP, and LL-37, and evaluated the therapeutic efficacy of the combination of BMAP-27 and LL-37 using a mouse pulmonary infection model. Our results showed that all of the four cathelicidins effectively killed bacteria via rapid induction of membrane permeabilization, and BMAP-27 exhibited the most excellent bactericidal activity against diverse bacterial pathogens. BMAP-27, mCRAMP, and LL-37 effectively inhibited biofilm formation, while BMAP-34, mCRAMP and LL-37 exerted immunomodulatory functions with varying degrees of efficacy by stimulating the chemotaxis of neutrophils, inducing the production of reactive oxygen species, and facilitating the formation of neutrophil extracellular traps. Of note, the combination of BMAP-27 and LL-37 effectively enhanced the clearance of Pseudomonas aeruginosa and reduced the organ injury in vivo. Together, these findings highlight that identifying the appropriate synergistic combination of mammalian cathelicidins with different beneficial properties may be an effective strategy against bacterial infection.

分类号:

  • 相关文献

[1]Antibacterial And Antibiofilm Activity Of L.actic Acid Bacteria Isolated F rom Traditional Artisanal Milk Cheese From Northeast China Against Enteropathogenic Bacteria. Cui, XY, Shi, YJ, Gu, SS, Yan, X, Chen, HY, Ge, JW. 2018

[2]The Cysteine Protease Apds From Streptococcus Suis Promotes Evasion Of Innate Immune Defenses By Cleaving The Antimicrobial Peptide Cathelicidin Ll-37. Xie, F, Zan, YN, Zhang, YL, Zheng, N, Yan, QL, Zhang, WJ, Zhang, HH, Jin, MJ, Chen, FG, Zhang, XY, Liu, SG. 2019

[3]Oxalic Acid From Sesbania Rostrata Seed Exudates Mediates The Chemotactic Response Of Azorhizobium Caulinodans Ors571 Using Multiple Strategies. Liu, XL, Zhang, KY, Liu, YN, Xie, ZH, Zhang, CS. 2019

[4]Pseudomonas Aeruginosa As A Powerful B.iofilm Producer And Positive A ction Of Amikacin Against Isolates From Chronic Wounds. Rahim, K, Saleha, S, Basit, A, Zhu, XD, Ahmed, I, Huo, L, Zhang, P, Usman, B, Munir, S, Franco, OL. 2017

[5]Antagonistic Activity And Mode Of A.ction Of Phenazine-1-Carboxylic Acid, P roduced By Marine Bacterium Pseudomonas Aeruginosa Pa31X, Against Vibrio Anguillarum In Vitro And In A Zebrafish In Vivo Model. Zhang, LL, Tian, XY, Kuang, S, Liu, G, Zhang, CS, Sun, CM. 2017

[6]Bio-Detoxification Bacteria Isolated From Dye-Polluted S.oils Promote Lactic Acid P roduction From Ammonia Pretreated Corn Stover. Liu, L, Cai, YY, Li, H, Zhao, SM, He, MX, Hu, GQ, Liang, YX, Peng, N, Hu, JL. 2019

[7]Whole Genome Analysis of Sugarcane Root-Associated Endophyte Pseudomonas aeruginosa B18—A Plant Growth-Promoting Bacterium With Antagonistic Potential Against Sporisorium scitamineum. Pratiksha Singh,Rajesh Kumar Singh,Dao-Jun Guo,Anjney Sharma,Ram Nageena Singh,Dong-Ping Li,Mukesh K. Malviya,Xiu-Peng Song,Prakash Lakshmanan,Li-Tao Yang,Yang-Rui Li. 2021

[8]Epsp Of L. Casei Bl23 P.rotected Against The Infection C aused By Aeromonas Veronii Via Enhancement Of Immune Response In Zebrafish. Qin, CB, Zhang, Z, Wang, YB, Li, SN, Ran, C, Hu, J, Xie, YD, Li, WF, Zhou, ZG. 2017

[9]Transcriptomic Responses Of Water Buffalo L.iver To Infection With T he Digenetic Fluke Fasciola Gigantica. Zhang, FK, Zhang, XX, Elsheikha, HM, He, JJ, Sheng, ZA, Zheng, WB, Ma, JG, Huang, WY, Guo, AJ, Zhu, XQ. 2017

[10]Immunomodulation effects of polyphenols from thinned peach treated by different drying methods on RAW264.7 cells through the NF-κB and Nrf2 pathways. Chongting Guo,Jinfeng Bi,Xuan Li,Jian Lyu,Xuan Liu,Xinye Wu,Jianing Liu. 2021

[11]Characterization Of Haemonchus Contortus Excretory/Secretory Antigen (Es-15) And Its Modulatory Functions On Goat Immune Cells In Vitro. Ehsan, M, Gadahi, JA, Hasan, MW, Haseeb, M, Ali, H, Yan, RF, Xu, LX, Song, XK, Zhu, XQ, Li, XR. 2020

[12]The Multitasking Fasciola Gigantica Cathepsin B. Interferes With Various F unctions Of Goat Peripheral Blood Mononuclear Cells In Vitro. Chen, D, Tian, AL, Hou, JL, Li, JX, Tian, XW, Yuan, XD, Li, XR, Elsheikha, HM, Zhu, XQ. 2019

[13]The Pervasive Effects Of Recombinant F.asciola Gigantica Ras-Related Protein R ab10 On The Functions Of Goat Peripheral Blood Mononuclear Cells. Tian, AL, Lu, MM, Zhang, FK, Calderon-Mantilla, G, Petsalaki, E, Tian, XW, Wang, WJ, Huang, SY, Li, XR, Elsheikha, HM, Zhu, XQ. 2018

[14]Impact Of Lactobacillus Casei Bl23 O.n The Host Transcriptome, G rowth And Disease Resistance In Larval Zebrafish. Qin, CB, Xie, YD, Wang, YB, Li, SN, Ran, C, He, SX, Zhou, ZG. 2018

[15]Extracellular Vesicles Derived Fromtrichinella Spiralismuscle Larvae Ameliorate Tnbs-Induced Colitis In Mice. Yang, Y, Liu, L, Liu, XL, Zhang, YY, Shi, HN, Jia, WZ, Zhu, HF, Jia, H, Liu, MY, Bai, X. 2020

[16]Biological Characteristics Of A New A.ntibacterial Peptide And Its A ntibacterial Mechanisms Against Gram-Negative Bacteria. Pei, Z.,Ying, X.,Tang, Y.,Kong, L.,Ma, H.,Liu, S.,Zhang, H.,Liu, L.,Wang, K.,Zhang, D.,Gao, Y.. 2018

[17]A novel PCR-based method for high throughput prokaryotic expression of antimicrobial peptide genes. Ke, Tao,Chen, Jibao,Liang, Su,Huang, Jin,Ma, Xiangdong,Kang, Jianxiong,Liu, Dongqi,Ke, Tao,Mao, Han,Dong, Caihua,Huang, Junyan,Liu, Shengyi. 2012

[18]Functional analysis of a novel cysteine-rich antimicrobial peptide from the salivary glands of the tick Rhipicephalus haemaphysaloides. Zhang, Houshuang,Yang, Siqi,Gong, Haiyan,Cao, Jie,Zhou, Yongzhi,Zhou, Jinlin,Zhou, Jinlin,Yang, Siqi.

[19]Sequence characterization and expression patterns of two defensin-like antimicrobial peptides from the tick Haemaphysalis longicornis. Zhou, Jinlin,Liao, Min,Ueda, Marni,Gong, Haiyan,Xuan, Xuenan,Fujisaki, Kozo. 2007

[20]Expression of antimicrobial peptide LH multimers in Escherichia coli C43(DE3). Tian, Zi-gang,Dong, Tian-tang,Yang, Ya-lin,Teng, Da,Wang, Jian-hua.

作者其他论文 更多>>