数字农科院2.0

Oocyte Ivm Or Vitrification Significantly Impairs Dna Methylation Patterns In Blastocysts As Analysed By Single-Cell Whole-Genome Methylation Sequencing

文献类型： 外文期刊

作者： Zhao, YH; Wang, JJ; Zhang, PP; Hao, HS; Pang, YW; Wang, HY; Du, WH; Zhao, SJ; Ruan, WM; Zou, HY; Hao, T; Zhu, HB; Zhao, XM

作者机构：

关键词： Bovine; Development; Embryo; Mechanism

期刊名称： REPRODUCTION FERTILITY AND DEVELOPMENT

ISSN： 1031-3613

年卷期： 2020 年 32 卷 7 期

页码：

收录情况： JCR（2021版）

摘要： To explore the mechanisms leading to the poor quality of IVF blastocysts, the single-cell whole-genome methylation sequencing technique was used in this study to analyse the methylation patterns of bovine blastocysts derived from in vivo, fresh (IVF) or vitrified (V_IVF) oocytes. Genome methylation levels of blastocysts in the IVF and V_IVF groups were significantly lower than those of the in vivo group (P < 0.05). In all, 1149 differentially methylated regions (DMRs) were identified between the IVF and in vivo groups, 1578 DMRs were identified between the V_IVF and in vivo groups and 151 DMRs were identified between the V_IVF and IVF groups. For imprinted genes, methylation levels of insulin-like growth factor 2 receptor (IGF2R) and protein phosphatase 1 regulatory subunit 9A (PPP1R9A) were lower in the IVF and V_IVF groups than in the in vivo group, and the methylation level of paternally expressed 3 (PEG3) was lower in the V_IVF group than in the IVF and in vivo groups. Genes with DMRs between the IVF and in vivo and the V_IVF and IVF groups were primarily enriched in oocyte maturation pathways, whereas DMR sbetween the V_IVF and in vivo groups were enriched in fertilisation and vitrification-vulnerable pathways. The results of this study indicate that differences in the methylation of critical DMRs may contribute to the differences in quality between in vitro- and in vivo-derived embryos.

分类号：

  • 相关文献

[1]Research Progress of PPR Proteins in RNA Editing, Stress Response, Plant Growth and Development. Qin T.,Zhao P.,Sun J.,Zhao Y.,Zhang Y.,Yang Q.,Wang W.,Chen Z.,Mai T.,Zou Y.,Liu G.,Hao W.. 2021

[2]橙汁非酶褐变机制及控制措施. 苏霞,吴厚玖. 2012

[3]牛结核病. 刘思国,宫强,王牟平,王春来,彭永刚. 2004

[4]转cry1C和cry2A不同抗虫基因水稻品种强非靶标害虫灰飞虱生物学特性的影响. 林克剑,李飞,侯茂林. 2011

[5]转crylAh基因玉米花粉对龟纹瓢虫生长发育和成虫移动能力的影响. 王振营,何康来,白树雄. 2011

[6]Developmental Potential Of Buffalo Embryos Cultured In Serum Free Culture System. Shahzad, Qaisar,Liang, Xianwei,Xu, Hui-Yan,Pu, Liping,Waqas, Muhammad,Wadood, Armughan Ahmed,Xie, Long,Lu, Ke-Huan,Lu, Yangqing. 2020

[7]High-Throughput Sequencing Of Small Rna T.ranscriptomes In Maize Kernel I dentifies Mirnas Involved In Embryo And Endosperm Development. Jiang, Haiyang,Zhu, Ming,Xing, Lijuan,Xu, Miaoyun,Zhang, Min,Wang, Lei,Zou, Junjie,Zhu, Ming,Li, Wenzong. 2017

[8]Vitrification Alters Cell Adhesion Related G.enes In Pre-Implantation Buffalo E mbryos: Protective Role Of Beta-Mercaptoethanol. Zheng, Hai-Ying,Yu, Nong-Qi,Yu, Nong-Qi,Yang, Chun-Yan,Yang, Chun-Yan,Yan, Sheng-Fei,Yan, Sheng-Fei,Shang, Jiang-Hua,Shang, Jiang-Hua,Moussa, Mahmoud,Moussa, Mahmoud,Moussa, Mahmoud,Li, Meng-Qi,Li, Meng-Qi,Huang, Jia-Xiang,Huang, Jia-Xiang,Zheng, Hai-Ying. 2019

[9]Analysis Of Promoter Activity Reveals T.hat Gmftl2 Expression Differs F rom That Of The Known Flowering Locus T Genes In Soybean. 张晓玫,陈福禄,傅永福. 2017

[10]The landscape of chromatin accessibility in skeletal muscle during embryonic development in pigs. Yue Jingwei,Hou Xinhua,Liu Xin,Wang Ligang,Gao Hongmei,Zhao Fuping,Shi Lijun,Shi Liangyu,Yan Hua,Deng Tianyu,Gong Jianfei,Wang Lixian,Zhang Longchao. 2021

[11]Focal Adhesion Kinase Ptk2 Autophosphorylation I.s Not Required For T he Activation Of Sodium-Hydrogen Exchange By Decreased Cell Volume In The Preimplantation Mouse Embryo. Fenelon, JC, Xu, BZ, Baltz, JM. 2019

[12]Developmental toxicity of TCBPA on the nervous and cardiovascular systems of zebrafish (Danio rerio): A combination of transcriptomic and metabolomics. Liu W., Pan Y., Yang L., Xie Y., Chen X., Chang J., Hao W., Zhu L., Wan B.. 2023

[13]Evolutionary divergence in embryo and seed coat development of U’s Triangle Brassica species illustrated by a spatiotemporal transcriptome atlas. Gao P., Quilichini T.D., Yang H., Li Q., Nilsen K.T., Qin L., Babic V., Liu L., Cram D., Pasha A., Esteban E., Condie J., Sidebottom C., Zhang Y., Huang Y., Zhang W., Bhowmik P., Kochian L.V., Konkin D., Wei Y., Provart N.J., Kagale S., Smith M., Patterson N., Gillmor C.S., Datla R., Xiang D.. 2022

[14]Environmental level of bisphenol F induced reproductive toxicity toward zebrafish. Mu X., Qi S., Liu J., Wang H., Yuan L., Qian L., Li T., Huang Y., Wang C., Guo Y., Li Y.. 2022

[15]Enhanced As(Iii) Removal From Aqueous S.olution By Fe-Mn-La-Impregnated Biochar C omposites. Qiu, Weiwen,Liu, Xuewei,Lin, Lina,Khan, Zulgarnain Haider,Song, Zhengguo. 2019

[16]Comparative Transcriptomic Analysis Of Biological P.rocess And Key Pathway I n Three Cotton (Gossypium Spp.) Species Under Drought Stress. Ma, Fanglu,Chen, Yadong,Li, Feng,Islam, Faisal,Mosfeq-Ul Hasan, Md,Shen, Hao,Sajid, Muhammad,Mosfeq-Ul Hasan, Md,Wang, Xuede,Prodhan, Zakaria H.. 2019

[17]Is There A Strategy I I.ron Uptake Mechanism In M aize?. Zhou, Xiaojin,Li, Suzhen,Chen, Jingtang,Li, Suzhen,Chen, Rumei. 2018

[18]Effects Of Low-Levels Of Three H.exabromocyclododecane Diastereomers On The M etabolic Profiles Of Pak Choi Leaves Using High-Throughput Untargeted Metabolomics Approach. Guo, Qiqi,Liu, Xiaowei,Wang, Yuehua,He, Zeying,Tan, Dongfei,Zhang, Yanwei. 2018

[19]Enhanced Removal Of Microcystis Aeruginosa I.n Bdd-Cf Electrochemical System B y Simple Addition Of Fe2+. Long, YJ, Li, HN, Xing, X, Ni, JR. 2017

[20]Insights Into Photocatalytic Inactivation Mechanism O.f The Hypertoxic Site I n Aflatoxin B1 Over Clew-Like Wo3 Decorated With Cds Nanoparticles. Mao, J, Li, PW, Wang, JM, Wang, HT, Zhang, Q, Zhang, LX, Li, H, Zhang, W, Peng, TY. 2019

作者其他论文 更多>>