数字农科院2.0

Ghrh32 Negatively Regulates Cold Tolerance In Upland Cotton (Gossypium Hirsutum L.)

文献类型： 外文期刊

作者： Ge, Chang-wei;Ge, Chang-wei;Ge, Chang-wei;Ge, Chang-wei;Yang, Yong-fei;Liu, Rui-hua;Ma, Hui-juan;Li, Yang;Zhao, Xin-hua;Pang, Chao-you;Yang, Yong-fei;Yang, Yong-fei;Zhou, Yu-qing;Wang, Li;Li, Shi-cong;Li, Shi-cong;Zhou, Yu-qing;Zhou, Yu-qing;Liu, Rui-hua;Zhang, Si-ping;Zhao, Xin-hua;Liu, Shao-dong;Pang, Chao-you;Chen, Jing;Shen, Qian

作者机构：

关键词： Cold; Dead-Box Rna Helicase; Upland Cotton; Cbf

期刊名称： PLANT GROWTH REGULATION

ISSN： 0167-6903

年卷期： 2020 年 91 卷 2 期

页码：

摘要： DEAD-box RNA helicases regulate growth, development, and stress response of plants. In this study, a gene encoding a DEAD-box RNA helicase, GhRH32, was identified in upland cotton (Gossypium hirsutum L.). Gene silencing experiments demonstrated that GhRH32 functions as a negative regulator in cold tolerance. ATPase activity and ATP content analysis revealed that GhRH32 regulates ATP supply. Subcellular localization analysis showed that GhRH32 is targeted to the nucleus. Furthermore, we found that GhRH32 interacts with GhCBF, and the expression of CBF pathway genes increased in plants that were subjected to virus induced gene silencing (VIGS). Taken together, our results indicate that GhRH32 could be a potential molecular target to improve cold tolerance in plants.

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