数字农科院2.0

A Novel Microfluidic Paper-Based Analytical Device Based On Chemiluminescence For The Determination Of Beta-Agonists In Swine Hair

文献类型： 外文期刊

作者： Li, W; Luo, Y; Yue, XQ; Wu, JR; Wu, RN; Qiao, Y; Peng, Q; Shi, B; Lin, BC; Chen, X

作者机构：

期刊名称： ANALYTICAL METHODS

ISSN： 1759-9660

年卷期： 2020 年 12 卷 18 期

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： beta-Agonists are illegal feed additives in the feed industries of many countries, especially China. Here, we report a microfluidic paper-based analytical device (mu PAD) coupled with the chemiluminescence (CL) method to provide the sensitive, simple and rapid quantitative detection of beta-agonists in swine hair samples. In this study, we found that the beta-agonists diminished the CL generated by the reaction of K-3[Fe(CN)(6)] and luminol on mu PAD, which was different from that observed in the aqueous solution, and the degree of diminishment was proportional to the concentration of beta-agonists. The possible mechanism was discussed as well. Also, this detection method showed a wide linear range (from 4.0 x 10(-8) to 1.0 x 10(-5) mol L-1) and low limit of detection (2.0 x 10(-8) mol L-1) with a low consumption of samples and reagents. Satisfactory recovery values (from 78% to 95%) were achieved. Therefore, our mu PAD CL sensor will be favorable to develop a miniaturized instrument for the on-site analysis of beta-agonists in swine hair samples.

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