数字农科院2.0

Formaldehyde Formation In The Glycine Cleavage System And Its Use For An Aldolase-Based Biosynthesis Of 1,3-Prodanediol

文献类型： 外文期刊

作者： Xu, YY; Meng, H; Ren, J; Zeng, AP

作者机构：

关键词： Glycine Cleavage System; Formaldehyde; 5; 10-Ch2-Thf; 1; 3; Propanediol

期刊名称： JOURNAL OF BIOLOGICAL ENGINEERING

ISSN： 1754-1611

年卷期： 2020 年 14 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： Glycine cleavage system (GCS) occupies a key position in one-carbon (C1) metabolic pathway and receives great attention for the use of C1 carbons like formate and CO2 via synthetic biology. In this work, we demonstrate that formaldehyde exists as a substantial byproduct of the GCS reaction cycle. Three causes are identified for its formation. First, the principal one is the decomposition of N-5,N-10-methylene-tetrahydrofolate (5,10-CH2-THF) to form formaldehyde and THF. Increasing the rate of glycine cleavage promotes the formation of 5,10-CH2-THF, thereby increasing the formaldehyde release rate. Next, formaldehyde can be produced in the GCS even in the absence of THF. The reason is that T-protein of the GCS can degrade methylamine-loaded H-protein (H-int) to formaldehyde and ammonia, accompanied with the formation of dihydrolipoyl H-protein (H-red), but the reaction rate is less than 0.16% of that in the presence of THF. Increasing T-protein concentration can speed up the release rate of formaldehyde by H-int. Finally, a certain amount of formaldehyde can be formed in the GCS due to oxidative degradation of THF. Based on a formaldehyde-dependent aldolase, we elaborated a glycine-based one carbon metabolic pathway for the biosynthesis of 1,3-propanediol (1,3-PDO) in vitro. This work provides quantitative data and mechanistic understanding of formaldehyde formation in the GCS and a new biosynthetic pathway of 1,3-PDO.

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