Development Of An Enzyme-Linked Immunosorbent Assay Based On Cd150/Slam For The Detection Of Peste Des Petits Ruminant Virus
文献类型: 外文期刊
作者: Prajapati, M; Dou, YX; Zhu, XL; Zhao, SY; Alfred, N; Li, YM; Zhang, ZD
作者机构:
关键词: Elisa; Ppr Virus; Slam (Cd150); Diagnosis; Rt-Qpcr
期刊名称: FRONTIERS IN VETERINARY SCIENCE
ISSN:
年卷期: 2020 年 7 卷
页码:
收录情况: JCR(2021版)
摘要: Peste des petits ruminant (PPR) is an economically important severe viral disease of small ruminants that affects primarily the respiratory and digestive tract. Specific detection of the PPR virus (PPRV) antigen plays an important role in the disease control and eradication program. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) based on the recombinant goat signaling lymphocyte activation molecule (SLAM) as the capture ligand was successfully developed for the detection of the PPRV antigen (PPRV SLAM-iELISA). The assay was highly specific for PPRV with no cross-reactions among foot and mouth disease virus, Orf virus, sheep pox virus, and goat pox virus and had a sensitivity with a detection limit of 1.56 x 10(1) TCID50/reaction (50 mu l). Assessment of 136 samples showed that the developed PPRV SLAM-iELISA was well correlated with real-time RT-qPCR assays and commercially available sandwich ELISA for detection of PPRV and showed relative sensitivity and specificity of 93.75 and 100.83%, respectively. These results suggest that the developed PPRV SLAM-iELISA is suitable for specific detection of the PPRV antigen. This study demonstrated for the first time that the goat SLAM, the cellular receptor for PPRV, can be used for the development of a diagnostic method for the detection of PPRV.
分类号:
- 相关文献
作者其他论文 更多>>
-
Fmd Vaccine Matching: Inter Laboratory Study For Improved Understanding Of R(1) Values
作者:Willems, T; De Vleeschauwer, A; Perez-Filgueira, M; Li, YM; Ludi, A; Lefebvre, D; Wilsden, G; Statham, B; Haas, B; Mattion, N; Robiolo, B; Perez, CB; Maradei, E; Smitsaart, E; La Torre, J; De Clercq, K
关键词:Foot-And-Mouth Disease Virus; Liquid Phase Blocking Elisa (Lpbe); Virus Neutralization Test (Vnt); Vaccine Matching; R(1)-Value
-
Enhancing Thermostability Of A Psychrophilic Alpha-Amylase By The Structural Energy Optimization In The Trajectories Of Molecular Dynamics Simulations
作者:Li, QB; Yan, YR; Liu, XQ; Zhang, ZD; Tian, J; Wu, NF
关键词:Cold-Adapted Alpha-Amylase; Thermostability; Mutant; Molecular Dynamics Simulations; Energy Calculation
-
Nlrp3 Inflammasome Activation By Foot-And-Mouth Disease Virus Infection Mainly Induced By Viral Rna And Non-Structural Protein 2B
作者:Zhi, XY; Zhang, Y; Sun, SQ; Zhang, ZH; Dong, H; Luo, X; Wei, YQ; Lu, ZJ; Dou, YX; Wu, R; Jiang, ZF; Weng, CJ; Seo, HS; Guo, HC
关键词:Fmdv; Fmdv Rna; Viroporin; 2B; Nlrp3 Inflammasome; Il-1 Beta
-
Expanding Diversity Of Susceptible Hosts In Peste Des Petits Ruminants Virus Infection And Its Potential Mechanism Beyond
作者:Dou, YX; Liang, ZX; Prajapati, M; Zhang, R; Li, YM; Zhang, ZD
关键词:Morbillivirus; peste des petits ruminants; peste des petits ruminants virus; susceptible hosts; expanding; potential mechanism
-
Protaetia Brevitarsis Larvae Can Feed On And Convert Spent Mushroom Substrate From Auricularia Auricula And Lentinula Edodes Cultivation
作者:Wei, PP; Li, YM; Lai, DQ; Geng, LL; Liu, CQ; Zhang, J; Shu, CL; Liu, RM
关键词:Spent Mushroom Substrate; Protaetia Brevitarsis Larvae; Organic Fertilizer; Frass; Organic Carbon
-
How To Increase Maize Production Without Extra Nitrogen Input
作者:Hou, P; Liu, Y; Liu, WM; Liu, GZ; Xie, RZ; Wang, KR; Ming, B; Wang, YH; Zhao, RL; Zhang, WJ; Wang, YJ; Bian, SF; Ren, H; Zhao, XY; Liu, P; Chang, JZ; Zhang, GH; Liu, JY; Yuan, LZ; Zhao, HY; Shi, L; Zhang, LL; Yu, L; Gao, JL; Yu, XF; Shen, LG; Yang, SZ; Zhang, ZD; Xue, JQ; Ma, XF; Wang, XQ; Lu, TQ; Dong, BC; Li, G; Ma, BX; Li, JQ; Deng, XF; Liu, YH; Yang, Q; Fu, H; Liu, XZ; Chen, XP; Huang, CL; Li, SK
关键词:Maize Production; Nitrogen; Density; Reactive N Losses Intensity; Ghg Intensity
-
The Mutation Of A Pectate Lyase-Like Gene Is Responsible For The Yellow Margin Phenotype In Potato
作者:Yang, ZM; Feng, SS; Tang, D; Zhang, LD; Li, YM; Kear, P; Huang, SW; Zhang, CZ
关键词: