数字农科院2.0

Global Proteomic Analysis Of Lysine Malonylation In Toxoplasma Gondii

文献类型： 外文期刊

作者： Nie, LB; Liang, QL; Du, R; Elsheikha, HM; Han, NJ; Li, FC; Zhu, XQ

作者机构：

关键词： Toxoplasma Gondii; Toxoplasmosis; Post-Translational Modifications; Lysine Malonylation; Malonylome

期刊名称： FRONTIERS IN MICROBIOLOGY

ISSN： 1664-302X

年卷期： 2020 年 11 卷

页码：

收录情况： JCR（2021版）

摘要： Lysine malonylation (Kmal) is a new post-translational modification (PTM), which has been reported in several prokaryotic and eukaryotic species. Although Kmal can regulate many and diverse biological processes in various organisms, knowledge about this important PTM in the apicomplexan parasite Toxoplasma gondii is limited. In this study, we performed the first global profiling of malonylated proteins in T. gondii tachyzoites using affinity enrichment and Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Three experiments performed in tandem revealed 294, 345, 352 Kmal sites on 203, 236, 230 malonylated proteins, respectively. Computational analysis showed the identified malonylated proteins to be localized in various subcellular compartments and involved in many cellular functions, particularly mitochondrial function. Additionally, one conserved Kmal motif with a strong bias for cysteine was detected. Taken together, these findings provide the first report of Kmal profile in T. gondii and should be an important resource for studying the physiological roles of Kmal in this parasite.

分类号：

  • 相关文献

[1]Immune Responses Induced By Pvax/Tgerk7 Against Toxoplasma Gondii Infection In Balb/C Mice. Guo, HT, Li, ZY, Wang, JL, Geng, ZY, Zhu, XQ. 2019

[2]Evaluation Of Protective Immunity Induced B.y Recombinant Calcium-Dependent Protein K inase 1 (Tgcdpk1) Protein Against Acute Toxoplasmosis In Mice. Huang, SY, Chen, K, Wang, JL, Yang, B, Zhu, XQ. 2019

[3]Immune Responses And Protection After D.na Vaccination Against Toxoplasma G ondii Calcium-Dependent Protein Kinase 2 (Tgcdpk2). Chen, K, Wang, JL, Huang, SY, Yang, WB, Zhu, WN, Zhu, XQ. 2017

[4]Evaluation Of Protective Immunity Induced B.y Dna Vaccination With G enes Encoding Toxoplasma Gondii Gra17 And Gra23 Against Acute Toxoplasmosis In Mice. Zhu, WN, Wang, JL, Chen, K, Yue, DM, Zhang, XX, Huang, SY, Zhu, XQ. 2017

[5]Sequence Variation In Rhoptry Neck P.rotein 10 Gene Among T oxoplasma Gondii Isolates From Different Hosts And Geographical Locations. Zhao, Y, Zhou, DH, Chen, J, Sun, XL. 2017

[6]Hydroxylamine And Carboxymethoxylamine Can Inhibit Toxoplasma Gondii Growth Through An Aspartate Aminotransferase-Independent Pathway. Li, JX, Guo, HP, Galon, EM, Gao, Y, Lee, SH, Liu, MM, Li, YC, Ji, SW, Jia, HL, Xuan, XN. 2020

[7]Malonylome Analysis In Developing Rice (.Oryza Saliva) Seeds Suggesting T hat Protein Lysine Malonylation Is Well-Conserved And Overlaps With Acetylation And Succinylation Substantially. Peng, Xiaojun,Peng, Zhaohua,Mujahid, Hana,Meng, Xiaoxi,Wang, Cailin,Xing, Shihai. 2018

[8]Prediction Of The Post-Translational Modifications O.f Adipokinetic Hormone Receptors F rom Solitary To Eusocial Bees. Yang, HP, Huang, JX, Liu, YJ, Li, JL, Luo, SD, Wu, J. 2018

[9]Comparison Of Predicted Amino Acid Sequences Of Allatotropin/Allatostatin Receptors From Solitary To Eusocial Bee Species (Hymenoptera, Apoidea). Yang, HP, Wu, J. 2019

[10]Practical Chemical Synthesis Of Atypical U.biquitin Chains By Using A n Isopeptide-Linked Ub Isomer. Tang, S, Liang, LJ, Si, YY, Gao, S, Wang, JX, Liang, J, Mei, ZQ, Zheng, JS, Liu, L. 2017

[11]Seroprevalence Of Toxoplasma Gondii In S.outh Asian Countries. Khan, MU, Rashid, I, Akbar, H, Islam, S, Riaz, F, Nabi, H, Ashraf, K, Singla, LD. 2017

[12]Effect of deletion of gra17 and gra23 genes on the growth, virulence and immunogenicity of type II Toxoplasma gondii.. . 2020

[13]Functional Characterization of Two Thioredoxin Proteins of Toxoplasma gondii Using the CRISPR-Cas9 System. Zhi-Wei Zhang,Ting-Ting Li,Jin-Lei Wang,Qin-Li Liang,Hai-Sheng Zhang,Li-Xiu Sun,Xing-Quan Zhu. 2021

[14]Seroprevalence Of Toxoplasma Gondii In Cattle In China From 2010 To 2019: A Systematic Review And Meta-Analysis. Gong, QL, Li, J, Li, D, Tian, T, Leng, X, Li, JM, Shi, K, Zhang, NZ, Du, R, Zhao, Q. 2020

[15]First Report of Toxoplasma gondii Seroprevalence in Pet Parrots in China. Zhang, Xiao-Xuan,Zhang, Nian-Zhang,Tian, Wei-Peng,Zhou, Dong-Hui,Zhu, Xing-Quan,Zhang, Xiao-Xuan,Xu, Ying-Tian,Tian, Wei-Peng. 2014

[16]Impact of environmental factors on the emergence, transmission and distribution of Toxoplasma gondii. Yan, Chao,Liang, Li-Jun,Zheng, Kui-Yang,Zhu, Xing-Quan,Yan, Chao,Zhu, Xing-Quan,Liang, Li-Jun,Zhu, Xing-Quan. 2016

[17]Genetic Diversity of Toxoplasma gondii Strains from Different Hosts and Geographical Regions by Sequence Analysis of GRA20 Gene. Ning, Hong-Rui,Xu, Qian-Ming,Zhu, Xing-Quan,Ning, Hong-Rui,Huang, Si-Yang,Wang, Jin-Lei,Zhu, Xing-Quan. 2015

[18]Geographical patterns of Toxoplasma gondii genetic diversity revealed by multilocus PCR-RFLP genotyping. Shwab, E. Keats,Majumdar, Debashree,Su, Chunlei,Zhu, Xing-Quan,Su, Chunlei,Pena, Hilda F. J.,Gennari, Solange M.,Dubey, Jitender P.. 2014

[19]Analysis of miRNA expression profiling in mouse spleen affected by acute Toxoplasma gondii infection. He, Jun-Jun,Ma, Jun,Wang, Jin-Lei,Xu, Min-Jun,Zhu, Xing-Quan,Ma, Jun,Xu, Min-Jun,Zhu, Xing-Quan.

[20]Vaccination with Toxoplasma gondii calcium-dependent protein kinase 6 and rhoptry protein 18 encapsulated in poly(lactide-co-glycolide) microspheres induces long-term protective immunity in mice. Zhang, Nian-Zhang,Xu, Ying,Wang, Meng,Chen, Jia,Huang, Si-Yang,Zhu, Xing-Quan,Xu, Ying,Gao, Qi,Huang, Si-Yang,Zhu, Xing-Quan. 2016

作者其他论文 更多>>