文献类型: 外文期刊
作者: Juan Zhao ; Abolore Adijat Ajadi ; Yifeng Wang ; Xiaohong Tong ; Huimei Wang ; Liqun Tang ; Zhiyong Li ; Yazhou Shu ; Xixi Liu ; Shufan Li ; Shuang Wang ; Wanning Liu ; Jian Zhang *
作者机构:
关键词: Rice (Oryza Sativa L; ); Lncrnas; Seed Development; Grain Size
期刊名称: Genes
ISSN: 2073-4425
年卷期: 2020 年 11 卷 3 期
页码:
收录情况: JCR(2021版)
摘要: Rice seed is a pivotal reproductive organ that directly determines yield and quality. Long non-coding RNAs (lncRNAs) have been recognized as key regulators in plant development, but the roles of lncRNAs in rice seed development remain unclear. In this study, we performed a paired-end RNA sequencing in samples of rice pistils and seeds at three and seven days after pollination (DAP) respectively. A total of 540 lncRNAs were obtained, among which 482 lncRNAs had significantly different expression patterns during seed development. Results from semi-qPCR conducted on 15 randomly selected differentially expressed lncRNAs suggested high reliability of the transcriptomic data. RNA interference of TCONS_00023703, which is predominantly transcribed in developing seeds, significantly reduced grain length and thousand-grain weight. These results expanded the dataset of lncRNA in rice and enhanced our understanding of the biological functions of lncRNAs in rice seed development
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