数字农科院2.0

Competitive Annealing Mediated Isothermal Amplification (Camp) For Rapid And Simple Detection Of Listeria Monocytogenes In Milk

文献类型： 外文期刊

作者： Li, W; Mao, R; Yue, XQ; Wu, JR; Wu, RN; Qiao, Y; Peng, Q; Shi, B; Luo, Y; Chen, X; Du, YG

作者机构：

关键词： Competitive Annealing Mediated Isothermal Amplification; L.Monocytogenes; Milk

期刊名称： FOOD CONTROL

ISSN： 0956-7135

年卷期： 2020 年 117 卷

页码：

收录情况： JCR（2021版）

摘要： Listeria monocytogenes (L. monocytogenes ) is widespread in nature and considered an important foodborne pathogen. And it can cause listeriosis, which can lead to miscarriage, neonatal death, septicemia and meningitis. Therefore, the appropriate detection methods are needed to effectively control and prevent this pathogenic bacterium. Competitive annealing mediated isothermal amplification (CAMP) is a novel nucleic acid-based detection technology that amplifies DNA with high sensitivity and specificity under isothermal conditions. The aim of this study was to develop a CAMP assay for rapid and simple detection of L. monocytogenes in milk. In this system, a pair of inner primers was designed to specifically target hlyA gene of L. monocytogenes. For further optimization, loop primers and outer primers were designed to accelerate CAMP reaction. Compared with conventional PCR method, the CAMP assay has the same specificity and higher sensitivity. All 8 strains of L. monocytogenes yielded positive results using the CAMP assay and showed no cross reaction with 26 tested non L. monocytogenes strains. The detection limit of the CAMP assay was 1.0 x 10(2) CFU/mL for pure bacterial, which was 10-fold more sensitive than PCR. Additionally, the CAMP method with the enrichment step could reliably detect L. monocytogenes in artificially contaminated milk samples at concentration as low as 1 CFU/mL. These results indicate the developed CAMP is a potential useful method for rapid detection of L. monocytogenes in milk samples.

分类号：

  • 相关文献

[1]The Simultaneous Detection of Multiple Antibiotics in Milk and Pork Based on an Antibody Chip Biosensor. Xiao J., Wei N., Wu S., Li H., Yin X., Si Y., Li L., Peng D.. 2022

[2]A Rapid And Simple Hplc-Fld S.creening Method With Quechers A s The Sample Treatment For The Simultaneous Monitoring Of Nine Bisphenols In Milk. Xiong, Lin,Gao, Ya-Qin,Yan, Ping,Chu, Min,Li, Wei-Hong,Li, Wei-Hong,Gao, Ya-Qin,Yang, Xiao-Ling,Xiong, Lin,Yan, Ping,Xiong, Lin,Xiong, Lin,Chu, Min,Yan, Ping,Yang, Xiao-Ling. 2018

[3]Multiresidue Analysis Of Glucocorticoids In M.ilk By Lc-Ms/Ms With L ow-Temperature Purification And Dispersive Solid-Phase Extraction. Ma, LL, Fan, XJ, Jia, L, Wang, JQ, Wang, SL, Zhao, LW. 2017

[4]Effect Of Broussonetia Papyrifera L. (.Paper Mulberry) Silage On D ry Matter Intake, Milk Composition, Antioxidant Capacity And Milk Fatty Acid Profile In Dairy Cows. Si, BW, Tao, H, Zhang, XL, Guo, JP, Cui, K, Tu, Y, Diao, QY. 2018

[5]A Dummy Molecularly Imprinted Solid-Phase E.xtraction Coupled With Liquid C hromatography-Tandem Mass Spectrometry For Selective Determination Of Four Pyridine Carboxylic Acid Herbicides In Milk. Tan, SJ, Yu, HL, He, YH, Wang, M, Liu, GQ, Hong, SH, Yan, FY, Wang, YL, Wang, MQ, Li, TF, Wang, J, Abd El-Aty, AM, Haclmuftuoglu, A, She, YX. 2019

[6]Quantitative Polymerase Chain Reaction Coupled W.ith Sodium Dodecyl Sulfate A nd Propidium Monoazide For Detection Of Viable Streptococcus Agalactiae In Milk. Zhao, YK, Chen, H, Li, HM, Cai, JX, Meng, L, Dong, L, Zheng, N, Wang, JQ, Wang, C. 2019

[7]A Metabolomics Approach To Characterize R.aw, Pasteurized, And Ultra-High T emperature Milk Using Ultra-Performance Liquid Chromatography-Quadrupole Time-Of-Flight Mass Spectrometry And Multivariate Data Analysis. Zhang, YD, Li, P, Zheng, N, Jia, ZW, Meruva, N, Ladak, A, Cleland, G, Wen, F, Li, SL, Zhao, SG, Wang, JQ. 2018

[8]Population Structure, Genetic Diversity And S.election Signatures Within Seven I ndigenous Pakistani Goat Populations. Kumar, C, Song, S, Dewani, P, Kumar, M, Parkash, O, Ma, Y, Malhi, KK, Yang, N, Mwacharo, JM, He, X, Jiang, L. 2018

[9]Presence Of Tetracyclines, Quinolones, Lincomycin A.nd Streptomycin In Milk. Du, BY, Wen, F, Zhang, YD, Zheng, N, Li, SL, Li, FD, Wang, JQ. 2019

[10]Accumulation And Depletion Of Cadmium I.n The Blood, Milk, H air, Feces, And Urine Of Cows During And After Treatment. Su, CY, Zhang, JM, Li, ZT, Zhao, QY, Liu, KD, Sun, YD, Wang, JH. 2017

[11]Elimination Of Ceftiofur Hydrochloride Residue I.n Postpartum Cows' Milk A fter Intramammary Infusing At Dry-Off. Kang, JJ, Liu, YM, Zhao, LL, Xu, F, Chen, XJ, Yan, X, Li, XB. 2018

[12]Multi-Mycotoxins Analysis In Raw Milk B.y Ultra High Performance L iquid Chromatography Coupled To Quadrupole Orbitrap Mass Spectrometry. Mao, JF, Zheng, N, Wen, F, Guo, LG, Fu, CP, Ouyang, HX, Zhong, LL, Wang, JQ, Lei, SR. 2018

[13]Multiple authentications of high-value milk by centrifugal microfluidic chip-based real-time fluorescent LAMP. Wenjie Yu,Yanjing Chen,Zhiying Wang,Lu Qiao,Ruibin Xie,Juan Zhang,Suying Bian,Hui Li,Yan Zhang,Ailiang Chen. 2021

[14]Microbiome And Metabolic Changes In Milk In Response To Artemisinin Supplementation In Dairy Cows. Hou, K, Tong, JJ, Zhang, H, Gao, S, Guo, YQ, Niu, H, Xiong, BH, Jiang, LS. 2020

[15]Development and evaluation of a holographic method for analysis of infant formula humanization based on the milk nutrient molecular dataset. Xiu H., Feng B., Liu Y., Bai X., Li Z., He B., Wen C., Wang Q., Zhang H., Wang H., Shao H., Wang Y., Zhang Y.. 2022

[16]Milk Powder Extraction: Optimization of Conditions for the Water-Soluble Metabolites by Proton Nuclear Magnetic Resonance ( 1 H-NMR). Dan Zhu,Alan Hayman,Russell Frew,Biniam Kebede,Gang Chen,Ian Stewart. 2021

[17]Polyethyleneimine‐modified magnetic carbon nanotubes as solid‐phase extraction adsorbent for the analysis of multi‐class mycotoxins in milk via liquid chromatography–tandem mass spectrometry. Nan Li,Jing Qiu,Yongzhong Qian. 2021

[18]Milk biomarkers in relation to inherent and external factors based on metabolomics. Dan Zhu,Biniam Kebede,Kiri McComb,Alan Hayman,Gang Chen,Russell Frew. 2021

[19]Fluorescence Immunoassay Through Histone-Ds-Poly(At)-Templated Copper N.anoparticles As Signal Transductors F or The Sensitive Detection Of Salmonella Choleraesuis In Milk. Xiong, Y, Zhang, KK, Gao, B, Wu, YQ, Huang, XL, Lai, WH, Xiong, YH, Liu, Y. 2019

[20]Analysis Of Long Non-Coding Rnas And Mrnas Associated With Lactation In The Crop Of Pigeons (Columba Livia). Ma, H, Ni, AX, Ge, PZ, Li, YL, Shi, L, Wang, PL, Fan, J, Isa, AM, Sun, YY, Chen, JL. 2020

作者其他论文 更多>>