数字农科院2.0

Perturbations In Glycerophospholipid Levels Of Pc12 Cells After Exposure To Pcb95 Based On Targeted Lipidomics Analysis

文献类型： 外文期刊

作者： Wang, XL; Xu, YY; Jia, Q; Song, X; Zhang, L; Zhang, W; Qian, YZ; Qiu, J

作者机构：

关键词： Pcb95; Pc12 Cells; Lipidomics; Glycerophospholipid Metabolism

期刊名称： COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY

ISSN： 1532-0456

年卷期： 2020 年 235 卷

页码：

收录情况： JCR（2021版）

摘要： Polychlorinated biphenyls (PCBs) are a group of organic chlorine chemicals that can induce various adverse health effects in animals and humans. The toxicology of PCBs is a significant public health concern because of their long-term presence in the environment. Among the 209 PCB congeners, PCB95 has been reported to be neurotoxic, however, there has been limited researches on evaluating whether and how PCB95 affects cellular lipids, the most abundant components of the brain. In this study, PCB95 was found to inhibit cell proliferation at concentrations of 0.1 mu M, 2 mu M and 10 mu M for 120 h. Additionally, there may be a shift in apoptosis to necrosis at 2 mu M PCB95 exposure for 24 h. However, lipid peroxidation was found not dominant for PCB95 exposure, especially at the concentrations of 0.1 mu M and 2 mu M. Because of playing vital roles in cell metabolism, 20 glycerophospholipids in PC12 cells were investigated after exposure to PCB95 for 120 h. The distinctions in the orthogonal projection to latent structures-discriminant analysis (OPLS-DA) models indicated that different concentrations of PCB95 leaded to aberrant glycerophospholipid metabolism. Based on the principles of t-test Pvalue < 0.05, variable importance at projection (VIP) value >1 and fold change >1, PC (14:0/14:0) and PC (16:0/14:0) were screened as potential biomarkers from all the target glycerophospholipids. This study is the first time that identifies the effects of PCB95 on specific glycerophospholipids in PC12 cells, and the observed changes in glycerophospholipids provides the basis for further evaluation of PCB95-induced neurotoxicity mechanisms.

分类号：

  • 相关文献

[1]Analysis Of Glycerophospholipid Metabolism After E.xposure To Pcb153 In P c12 Cells Through Targeted Lipidomics By Uhplc-Ms/Ms. Wang, XL, Xu, YY, Song, X, Jia, Q, Zhang, XN, Qian, YZ, Qiu, J. 2019

[2]Lipidomic profile changes of yellow-feathered chicken meat during thermal processing based on UPLC-ESI-MS approach. Zhang M., Xie D., Wang D., Xu W., Zhang C., Li P., Sun C.. 2023

[3]Cytotoxicity Of 2,2 ',3,5 ',6-Pentachlorobiphenyl (Pcb95) And Its Metabolites In The Chicken Embryo Liver Cells Of Laying Hens. Liao, GQ, Song, X, Wang, XL, Zhang, W, Zhang, L, Qiu, J, Hou, RY. 2020

[4]Involvement Of Phosphatidylserine And Triacylglycerol I.n The Response Of S weet Potato Leaves To Salt Stress. Li, Zongyun,Xuan, Ying,Yu, Yicheng,Sun, Jian,Tang, Zhonghou,Liu, Yang,Gao, Zhonghui,Liu, Yaju,Cao, Qinghe,Kou, Meng. 2019

[5]Investigation of the lipidomic profile of royal jelly from different botanical origins using UHPLC-IM-Q-TOF-MS and GC-MS. Yan S., Wang X., Sun M., Wang W., Wu L., Xue X.. 2022

[6]Lipidomics provides novel insights into understanding the bee pollen lipids transepithelial transport and metabolism in human intestinal cells. 李强强,,薛晓锋,,王凯,,吴黎明. 2020

[7]Lipidomes In Health And Disease: Analytical Strategies And Considerations. Wei, F, Lamichhane, S, Oresic, M, Hyotylainen, T. 2019

[8]Uplc-Q-Exactive Orbitrap/Ms-Based Lipidomics Approach To C.haracterize Lipid Extracts From B ee Pollen And Their In Vitro Anti-Inflammatory Properties. Li, QQ, Liang, XW, Zhao, L, Zhang, ZY, Xue, XF, Wang, K, We, LM. 2017

[9]Characterization And Discrimination Of Taihe B.lack-Boned Silky Fowl (Gallus G allus Domesticus Brisson) Muscles Using Lc/Ms-Based Lipidomics. Mi, S, Shang, K, Jia, W, Zhang, CH, Li, X, Fan, YQ, Wang, H. 2018

[10]Methods of Lipidomic Analysis: Extraction, Derivatization, Separation, and Identification of Lipids. Xie Ya,Wu Zongyuan,Qin Zuojian,Wu Bangfu,Lv Xin,Wei Fang,Chen Hong. 2021

[11]Investigation on green tea lipids and their metabolic variations during manufacturing by nontargeted lipidomics. Jia Li,Jinjie Hua,Haibo Yuan,Yuliang Deng,Qinghua Zhou,Yanqin Yang,Chunwang Dong,Jun Zeng,Yongwen Jiang. 2021

[12]Comprehensive Lipidomics Analysis Reveals The Effects Of Different Omega-3 Polyunsaturated Fatty Acid-Rich Diets On Egg Yolk Lipids. Wu, BF, Xie, Y, Xu, SL, Lv, X, Yin, HQ, Xiang, JQ, Chen, H, Wei, F. 2020

[13]Characterization of key lipids for binding and generating aroma compounds in roasted mutton by UPLC-ESI-MS/MS and Orbitrap Exploris GC. Liu H., Hui T., Zheng X., Li S., Wei X., Li P., Zhang D., Wang Z.. 2022

[14]Serumal Lipidomics Reveals the Anti-inflammatory Effect of Flax Lignans and Sinapic Acid in High-Fat-Diet-Fed Mice. Zongyuan Wu,Bangfu Wu,Xin Lv,Ya Xie,Shuling Xu,Congcong Ma,Jiqu Xu,Xinghao Tu,Fang Wei,Hong Chen. 2021

[15]Linking Gut Microbiome And Lipid Metabolism: Moving Beyond Associations. Lamichhane, S, Sen, P, Alves, MA, Ribeiro, HC, Raunioniemi, P, Hyotylainen, T, Oresic, M. 2021

[16]Lipidomics Strategy for the Identification of Ultra-High Temperature and Reconstituted Milk by UPLC-Q-Exactive Orbitrap Mass Spectrometry. Jing Cui,Dan Zhu,Meicheng Su,Dongfei Tan,Xia Zhang,Man Jia,Huaxing Wu,Gang Chen. 2021

[17]Response of lipid molecular structure to dietary lipid type in Chinese mitten crab Eriocheir sinensis: A deep lipidomics analysis. Chang Xu,Qianqian Ma,Erchao Li,Yan Liu,Xiaodan Wang,Zhenyu Du,Jian G. Qin,Liqiao Chen. 2021

[18]Angustoline Inhibited Esophageal Tumors Through Regulating Lkb1/Ampk/Elavl1/Lpact2 Pathway And Phospholipid Remodeling. Li, HY, Zhang, C, Zhang, M, Yao, QQ, Yang, HG, Fan, LL, Zheng, N. 2020

[19]Stereoselective Bioaccumulation Of Chiral Pcb 9.1 In Earthworm And I ts Metabolomic And Lipidomic Responses. He, ZY, Wang, YH, Zhang, YW, Cheng, HY, Liu, XW. 2018

[20]Adipose Lipidomics And Rna-Seq Analysis R.evealed The Enhanced Mitochondrial F unction In Ucp1 Knock-In Pigs. Pan, JF, Tao, C, Cao, CW, Zheng, QT, Lam, SM, Shui, GH, Liu, XX, Li, K, Zhao, JG, Wang, YF. 2019

作者其他论文 更多>>