数字农科院2.0

Microrna858 Negatively Regulates Anthocyanin Biosynthesis By Repressing Aamybc1 Expression In Kiwifruit (Actinidia Arguta)

文献类型： 外文期刊

作者： Li, YK; Cui, W; Qi, XJ; Lin, MM; Qiao, CK; Zhong, YP; Hu, CG; Fang, JB

作者机构：

关键词： Anthocyanin; Aamybc1; Mir858; Overexpression; Actinidia Arguta

期刊名称： PLANT SCIENCE

ISSN： 0168-9452

年卷期： 2020 年 296 卷

页码：

收录情况： JCR（2021版）

摘要： The anthocyanin biosynthetic pathway regulated by exogenous and endogenous factors through sophisticated networks has been extensively studied in kiwifruit (Actinidia arguta). However, the role of micro RNAs (miRNAs) as regulatory factor in this process is largely unclear. Here, we demonstrate that miR858 is a negative regulator of anthocyanin biosynthesis by repressing the target gene AaMYBC1 in red-colored kiwifruit. Transient co-transformation in Nicotiana benthamiana confirmed that miR858 could target AaMYBC1, which was identified to be an R2R3-type tanscription factor (TF). Subcellular localization showed that AaMYBC1 was located in the nucleus, indicating AaMYBC1 protein could act as a transcriptional regulator in plant cells. Functional protein association network analysis and the yeast two hybrid (Y2H) assay revealed that AaMYBC1 and AabHLH42 interact with each other. Silencing of AaMYBC1 using the virus-induced gene silencing method in the core of A. arguta 'HB' ('Hongbaoshixing', a kind of red-fleshed A. arguta cultivar) fruits reduced the accumulation of anthocyanin and decreased the expression of late biosynthetic genes. miR858 overexpression played a stronger role than AaMYBC1 silencing in the inhibition of coloration. With overexpression of miR858, A. arguta did not present coloration, and anthocyanin was hardly detected. Together, these results clarify the negative regulatory role of miR858 in mediating anthocyanin biosynthesis and accumulation in A. arguta, providing novel insights into the molecular mechanism of anthocyanin biosynthesis.

分类号：

  • 相关文献

[1]Combined Analysis Of The Fruit M.etabolome And Transcriptome Reveals C andidate Genes Involved In Flavonoid Biosynthesis In Actinidia Arguta. Li, YK, Fang, JB, Qi, XJ, Lin, MM, Zhong, YP, Sun, LM, Cui, W. 2018

[2]Sucrose Synthase Genes: A Way F.orward For Cotton Fiber I mprovement. Ahmed, Mukhtar,Sarwar, Muhammad Bilal,Rao, Abdul Qayyum,Ahmed, Mukhtar,Ahmed, Mukhtar,Ahmed, Mukhtar,Wang Xuede,Akhtar, Sidra,Latif, Ayesha,Husnain, Tayyab,Shahid, Ahmad Ali,Ma Fanglu,Din, Salah Ud. 2018

[3]Isolation, Transformation And Overexpression Of S.ugarcane Sop5Cs Gene For D rought Tolerance Improvement. Li, Yang-Rui,Li, Jian,Yang, Li-Tao,Thi-Thu Phan,Xing, Yong-Xiu. 2018

[4]Atp-Citrate Lyase Gene (Soacla-1), A N.ovel Acla Gene In S ugarcane, And Its Overexpression Enhance Drought Tolerance Of Transgenic Tobacco. Thi-Thu Phan,Sun, Bo,Yang, Li-Tao,Zhao, Wen-Hui,Yang, Li-Tao,Li, Jian,Huang, Chan,Li, Yang-Rui,Liu, Jia-Yi,Li, Yang-Rui. 2017

[5]Overexpression Of The Peat1 Elicitor G.ene From Alternaria Tenuissima I mproves Drought Tolerance In Rice Plants Via Interaction With A Myo-Inositol Oxygenase. Shi, Fachao,Yang, Xiufeng,Zhang, Yi,Dong, Yijie,Qiu, Dewen,Shi, Fachao. 2017

[6]Overexpression Of Bnkcs1-1, Bnkcs1-2, And Bncer1-2 Promotes Cuticular Wax Production And Increases Drought Tolerance In Brassica Napus. Li, Shuai,Xu, Yi,Jin, Shurong,Zhang, Shuangjuan,Ni, Yu,Li, Jiana,Yuan, Zheng,Wang, Yanmei. 2020

[7]Roles Of Soybean Plasma Membrane I.ntrinsic Protein Gmpip2;9 In D rought Tolerance And Seed Development. Liu, Ruifang,Shou, Huixia,Lu, Linghong,Wang, Chuang,Lu, Linghong,Dong, Changhe,Zhou, Bin,Wang, Chuang. 2018

[8]Characterization Of Transcription Factor Gene &.Itosdrap1&It Conferring Drought Tolerance I n Rice. Huang, Liyu,Huang, Liyu,Li, Zhikang,Wang, Wensheng,Zhao, Yan,Zhao, Xiuqin,Wang, Yinxiao,Qin, Qiao,Li, Zichao,Li, Zhikang,Sun, Fan,Fu, Binying,Fu, Binying,Hu, Fengyi. 2018

[9]Improving Zinc And Iron Accumulation I.n Maize Grains Using T he Zinc And Iron Transporter Zmzip5. Chen, Rumei,Yang, Wenzhu,Li, Ye,Liu, Xiaoqing,Li, Suzhen,Zhou, Xiaojin. 2019

[10]Genome-Wide Analysis Of The Nf-Yb G.ene Family In Gossypium H irsutum L. And Characterization Of The Role Of Ghdnf-Yb22 In Embryogenesis. Ge, Xiaoyang,Yang, Zhaoen,Li, Fuguang,Yang, Zhaoen,Chen, Yanli,Xiao, Yanqing,Chen, Yanli,Zhang, Chaojun,Zhang, Xianlong,Wang, Ye,Wang, Peng. 2018

[11]The Role Of Gibberellin Synthase G.ene Ghga2Ox1 In Upland C otton (Gossypium Hirsutum L.) Responses To Drought And Salt Stress. Yan, Gen-Tu,Zhou, Hong,Xu, Qing-Hua,Wang, Ning,Shi, Jian-Bin. 2019

[12]Fatty Acid Biosynthesis And Transcriptional Regulation Of Stearoyl-Coa Desaturase 1 (Scd1) In Buffalo Milk. Rehman, Saif Ur,Shafique, Laiba,Luo, Chan,Liu, Qingyou,Wang, Zhiqiang,Cui, Kuiqing,Qian, Qian,Ruan, Jue,Liu, Qingyou,Lu, Suyu,Li, Zhipeng. 2020

[13]Expression Patterns Of Two Pal G.enes Of Pleurotus Ostreatus A cross Developmental Stages And Under Heat Stress. Hou, LD, Wang, LN, Wu, XL, Gao, W, Zhang, JX, Huang, CY. 2019

[14]Pro-197-Ser Mutation Inalsand High-Level Gst Activities: Multiple Resistance To Als And Accase Inhibitors Inbeckmannia Syzigachne. Wang, JJ, Chen, JC, Li, XJ, Li, D, Li, Z, Cui, HL. 2020

[15]Overexpression Ofwysrgene Enhances Wuyiencin Production In Delta Wysr3Mutant Strain Ofstreptomyces Albulusvar. Wuyiensis Strain Ck-15. Wei, Q, Aung, A, Liu, B, Ma, J, Shi, L, Zhang, K, Ge, B. 2020

[16]Transcriptome Analysis Reveals Cotton (Gossypium H.irsutum) Genes That Are D ifferentially Expressed In Cadmium Stress Tolerance. Han, MG, Lu, XK, Yu, J, Chen, XG, Wang, XG, Malik, WA, Wang, JJ, Wang, DL, Wang, S, Guo, LX, Chen, C, Cui, RF, Yang, XM, Ye, WW. 2019

[17]Transcriptome profiling and functional analysis suggest that the constitutive overexpression of four cytochrome P450s confers resistance to abamectin in Tetranychus urticae from China. Dandan Xu,Yan Zhang,Youjun Zhang,Qingjun Wu,Zhaojiang Guo,Wen Xie,Xiaomao Zhou,Shaoli Wang. 2021

[18]Overexpression Of Stcys1 Gene Enhances Tolerance To Salt Stress In The Transgenic Potato (Solanum Tuberosum L.) Plant. Liu, MM, Li, YL, Li, GC, Dong, TT, Liu, SY, Liu, P, Wang, QG. 2020

[19]Identification And Functional Characterization Of Nbmlp28, A Novel Mlp-Like Protein 28 Enhancing Potato Virus Y Resistance In Nicotiana Benthamiana. Song, LY, Wang, J, Jia, HY, Kamran, A, Qin, YX, Liu, YJ, Hao, KQ, Han, F, Zhang, CQ, Li, B, Li, YL, Shen, LL, Wang, FL, Wu, YH, Yang, JG. 2020

[20]Transcriptome profiling and functional analysis suggest that the constitutive overexpression of four cytochrome P450s confers resistance to abamectin in Tetranychus urticae from China. Dandan Xu,Yan Zhang,Youjun Zhang,Qingjun Wu,Zhaojiang Guo,Wen Xie,Xiaomao Zhou,Shaoli Wang. 2021

作者其他论文 更多>>