数字农科院2.0

Hs1 Is Involved In Hygromycin Resistance Through Facilitating Hygromycin Phosphotransferase Transportation From Cytosol To Chloroplast

文献类型： 外文期刊

作者： 罗彦忠; Zhang, L; Li, WW; Xu, MY; Zhang, CY; 王磊

作者机构：

关键词： Arabidopsis Thaliana; Chloroplast; Hs1; Hpt; Transit Peptide-Less Protein; Transportation; Hygromycin Sensitivity

期刊名称： FRONTIERS IN PLANT SCIENCE

ISSN： 1664-462X

年卷期： 2020 年 11 卷

页码：

收录情况： JCR（2021版）

摘要： The transportation of proteins encoded by nuclear genes from plant cytosol to chloroplast is essential for chloroplast functions. Proteins that have a chloroplast transit peptide (cTP) are imported into chloroplasts via translocases on the outer and inner chloroplast envelope. How proteins lacking transit sequence are imported into chloroplast remains largely unknown. During screening of an Arabidopsis population transformed with a hairpin RNA gene-silencing library, we identified some transgenic plants that had active expression of the selectable marker gene, hygromycin phosphotransferase (HPT), but were sensitive to the selection agent, hygromycin B (HyB). Mutant and complementation analysis showed that this HyB sensitivity of transgenic plants was due to silencing of the HS1 (Hygromycin-Sensitive 1) gene. HS1 is localized in the chloroplast and interacts physically with HPT in yeast cells and in planta. Fluorescence and immunoblotting analysis showed that HPT could not be transported effectively into chloroplasts in Aths1, which resulted in Aths1 is sensitivity to hygromycin on higher HyB-containing medium. These data revealed that HS1 is involved in HyB resistance in transgenic Arabidopsis through facilitating cytosol-chloroplast transportation of HPT. Our findings provide novel insights on transportation of chloroplast cTP-less proteins.

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