数字农科院2.0

Comparative Proteomics Analysis Indicates That Palmatine Contributes To Transepithelial Migration By Regulating Cellular Adhesion

文献类型： 外文期刊

作者： Yang, Feng;Xin, Ruihua;Wang, Hui;Sun, Yan;Cui, Dongan;He, Jiongjie;Zhang, Shidong

作者机构：

关键词： Proteomics; Mucosal Inflammation; Cell Adhesion Molecules

期刊名称： PHARMACEUTICAL BIOLOGY

ISSN： 1388-0209

年卷期： 2020 年 58 卷 1 期

页码：

摘要： Context Palmatine, a biologically active isoquinoline alkaloid, possesses multiple pharmaceutical activities against mucosal infection and inflammation. Objective There are no reports about the influence of palmatine on uterine mucosal epithelial cells. Materials and methods We used proteomics to analyse differentially expressed proteins (DEPs) in goat endometrial epithelial cells (EECs) stimulated by lipopolysaccharide (LPS, 5 mu g/mL, the dosage can induce inflammatory response, according to our previous study) for 12 h and then treated with palmatine (80 mu g/mL) for 8 h; the dosage was selected based on MTT assay. The EECs without any treatment were used as controls. Every group was treated in triplicate. Results A total of 428 DEPs in LPS-stimulated group and 486 DEPs in the palmatine-treated group were identified. Functional annotation analysis showed that palmatine mainly regulated the protein expression of structural molecules involved in the response to stimuli. Pathway analysis showed that cell adhesion molecule (CaM) pathways were most significant enriched due to palmatine treatment. Junction adhesion molecule 1 (JAM1), nectin 1 (NECT1) and cadherin 5 (CDH5), which play important roles in the transepithelial migration (TEpM) of leukocytes, were significantly downregulated by palmatine. Meanwhile, other proteins essential to the maintenance of cell adhesion and those that facilitate leukocyte migration were upregulated after palmatine treatment. Discussion and conclusions:The results suggested that palmatine regulates the expression of CaMs to affect TEpM during uterine mucosal inflammation and provides novel insight to understanding and developing palmatine pharmacology. Palmatine is a promising drug for treatment of mucosal inflammation.

分类号：

  • 相关文献

[1]Alterations In The Mucosa-Associated Fungal M.icrobiota In Patients With U lcerative Colitis. Qiu, XY, Ma, JJ, Jiao, CH, Mao, XQ, Zhao, XJ, Lu, MJ, Wang, K, Zhang, HJ. 2017

[2]Tembusu Virus Entering the Central Nervous System Caused Nonsuppurative Encephalitis without Disrupting the Blood-Brain Barrier. Sheng Yang,Yufei Huang,Yonghong Shi,Xuebing Bai,Ping Yang,Qiusheng Chen. 2021

[3]Comparative proteomics analysis of pomegranate seeds on fruit maturation period (Punica granatum L.). CAO Shang-yin,Niu Juan,Cao Da,Li Haoxian,Xue Hui,Chen Lina,Zhang Fuhong,ZHAO Di-guang. 2015

[4]PROTEOMIC ANALYSIS OF NOVEL Cry1Ac BINDING PROTEINS IN Helicoverpa armigera (HUBNER). Chen, Li-Zhen,Liang, Ge-Mei,Zhang, Jie,Wu, Kong-Ming,Guo, Yu-Yuan,Rector, Brian G.. 2010

[5]Comparative Proteomic Analysis Shows an Elevation of Mdh1 Associated with Hepatotoxicity Induced by Copper Nanoparticle in Rats. Dong Shu-wei,Gao Zhao-hui,Li Xia,Shen Xiao-yun,Gao Zhao-hui,Xue Hui-wen. 2014

[6]Proteomics analysis of Xenopus laevis gonad tissue following chronic exposure to atrazine. Chen, Xiuping,Wang, Jiamei,Zhu, Haojun,Peng, Yufa,Wang, Jiamei,Zhu, Haojun,Ding, Jiatong. 2015

[7]Comparative Proteome Analysis of Two Antagonist Bacillus subtilis Strains. Zhang, C. X.,Zhao, X.,Han, F.,Yang, M. F.,Chen, H.,Shen, S. H.,Zhang, C. X.,Chida, T.. 2009

[8]Metabolic responses and "omics" technologies for elucidating the effects of heat stress in dairy cows. Min, Li,Zhao, Shengguo,Tian, He,Zhou, Xu,Zhang, Yangdong,Li, Songli,Zheng, Nan,Wang, Jiaqi,Min, Li,Yang, Hongjian.

[9]Twenty-six circulating antigens and two novel diagnostic candidate molecules identified in the serum of canines with experimental acute toxoplasmosis. Xue, Junxin,Xiao, Yan,Huang, Kehe,Xue, Junxin,Jiang, Wei,Chen, Yongjun,Liu, Yingchun,Zhang, Huajing,Wang, Quan,Qiao, Yuanbiao. 2016

[10]A genotypic difference in primary root length is associated with the inhibitory role of transforming growth factor-beta receptor-interacting protein-1 on root meristem size in wheat. He, Xue,Fang, Jingjing,Li, Jingjuan,Qu, Baoyuan,Ren, Yongzhe,Ma, Wenying,Zhao, Xueqiang,Li, Bin,Wang, Daowen,Li, Zhensheng,Tong, Yiping,Fang, Jingjing,Li, Jingjuan,Ren, Yongzhe. 2014

[11]iTRAQ-based quantitative proteomic analysis of longissimus muscle from growing pigs with dietary supplementation of non-starch polysaccharide enzymes. Zhang, Ji-ze,Gao, Yang,Lu, Qing-ping,Sa, Ren-na,Zhang, Hong-fu,Gao, Yang. 2015

[12]Proteomic Analysis of the Secretome of Porcine Alveolar Macrophages Infected with Porcine Reproductive and Respiratory Syndrome Virus. Li, Yuming,Wu, Zhuanchang,Liu, Ke,Qi, Pengfei,Xu, Jinpeng,Wei, Jianchao,Li, Beibei,Shao, Donghua,Shi, Yuanyuan,Qiu, Yafeng,Ma, Zhiyong.

[13]Proteomic analysis of castor bean tick Ixodes ricinus: a focus on chemosensory organs. Dani, Francesca Romana,Ban, Liping,Song, Limei,Pelosi, Paolo,Dani, Francesca Romana.

[14]Global iTRAQ-based proteomic profiling of Toxoplasma gondii oocysts during sporulation. Zhou, Chun-Xue,Suo, Xun,Zhou, Chun-Xue,Suo, Xun,Zhou, Chun-Xue,Zhu, Xing-Quan,He, Shuai,Zhou, Dong-Hui,Elsheikha, Hany M.,He, Shuai,Li, Qian.

[15]Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus. Deng, Xiaoyu,Cong, Yanlong,Yin, Renfu,Ding, Zhuang,Yang, Guilian,Wang, Chunfeng,Ding, Chan,Yu, Shengqing,Liu, Xiufan,Wang, Chunfeng.

[16]PROTEOMICS ANALYSIS OF OVEREXPRESSED PLASMA PROTEINS IN RESPONSE TO COLD ACCLIMATION IN Ostrinia furnacalis. Shang, Qingli,Pan, Yiou,Peng, Tianfei,Yang, Shuang,Xi, Jinghui,Lu, Xin,Wang, Zhenying.

[17]Comparative proteomics analysis of pomegranate seeds on fruit maturation period (Punica granatum L.). Cao Shang-yin,Niu Juan,Li Hao-xian,Xue Hui,Chen Li-na,Zhang Fu-hong,Zhao Di-guang,Cao Da. 2015

[18]Hypothalamic protein profiles associated with inhibited feed intake of ducks fed with insufficient dietary arginine. Wang, C.,Xie, M.,Huang, W.,Xie, J. J.,Hou, S. S.,Wang, C.,Zheng, A. J..

[19]Comparative proteomic analysis of alfalfa revealed new salt and drought stress-related factors involved in seed germination. Ma, Qiaoli,Kang, Junmei,Zhang, Kun,Wang, Tenghua,Sun, Yan,Kang, Junmei,Long, Ruicai,Zhang, Tiejun,Yang, Qingchuan,Ma, Qiaoli,Xiong, Junbo.

[20]Comparative proteomic analyses reveal the changes of metabolic features in soybean (Glycine max) pistils upon pollination. Li, Ming,Yang, Pingfang,Li, Ming,Sha, Aihua,Zhou, Xinan.

作者其他论文 更多>>