数字农科院2.0

The Trichome-Specific Acetolactate Synthase Ntals1 Gene, Is Involved In Acylsugar Biosynthesis In Tobacco (Nicotiana Tabacuml.)

文献类型： 外文期刊

作者： Chang, AX; Chen, B; Yang, AG; Hu, RS; Feng, QF; Chen, M; Yang, XN; Luo, CG; Li, YY; Wang, YY

作者机构：

关键词： Acylsugar; Ntals1; Tobacco; Acetolactate Synthase

期刊名称： PLANTA

ISSN： 0032-0935

年卷期： 2020 年 252 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： Main conclusion NtALS1is specifically expressed in glandular trichomes, and can improve the content of acylsugars in tobacco. Abtract The glandular trichomes of many species in the Solanaceae family play an important role in plant defense. These epidermal outgrowths exhibit specialized secondary metabolism, including the production of structurally diverse acylsugars that function in defense against insects and have substantial developmental potential for commercial uses. However, our current understanding of genes involved in acyl chain biosynthesis of acylsugars remains poor in tobacco. In this study, we identified three acetolactate synthase (ALS) genes in tobacco through homology-based gene prediction using ArabidopsisALS. Quantitative real-time PCR (qRT-PCR) and tissue distribution analyses suggested thatNtALS1was highly expressed in the tips of glandular trichomes. Subcellular localization analysis showed that the NtALS1 localized to the chloroplast. Moreover, in the wild-type K326 variety background, we generated twontals1loss-of-function mutants using the CRISPR-Cas9 system. Acylsugars contents in the twontals1mutants were significantly lower than those in the wild type. Through phylogenetic tree analysis, we also identifiedNtALS1orthologs that may be involved in acylsugar biosynthesis in other Solanaceae species. Taken together, these findings indicate a functional role forNtALS1in acylsugar biosynthesis in tobacco.

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