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Dendrigraft Poly-L-Lysines Delivery Of Dna Vaccine Effectively Enhances The Immunogenic Responses Against H9N2 Avian Influenza Virus Infection In Chickens

文献类型: 外文期刊

作者: Zhao, K; Rong, GY; Teng, QY; Li, XS; Lan, HL; Yu, L; Yu, S; Jin, Z; Chen, GP; Li, ZJ

作者机构:

关键词: H9N2 Avian Influenza; Dendrigraft Poly-L-Lysine; Nanoparticles; Delivery Of Dna Vaccine; Immune Responses

期刊名称: NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE

ISSN: 1549-9634

年卷期: 2020 年 27 卷

页码:

收录情况: JCR(2021版) ; EI(2021版)

摘要: Biodegradable nanomaterials can protect antigens from degradation, promote cellular absorption, and enhance immune responses. We constructed a eukaryotic plasmid [pCAGGS-opti441-hemagglutinin (HA)] by inserting the optimized HA gene fragment of H9N2 AIV into the pCAGGS vector. The pCAGGS-opti441-HA/DGL was developed through packaging the pCAGGS-opti441-HA with dendrigraft poly-llysines (DCiLs). DGL not only protected the pCAGGS-opti441 -HA from degradation, but also exhibited high transfection efficiency. Strong cellular immune responses were induced in chickens immunized with the pCAGGS-opti441-HA/DGL. The levels of IFN-gamma and IL-2, and lymphocyte transformation rate of the vaccinated chickens increased at the third week post the immunization. For the vaccinated chickens, T lymphocytes were activated and proliferated, the numbers of CD3+CD4+ and CD4+/CD8+ increased, and the chickens were protected completely against H9N2 MV challenge. This study provides a method for the development of novel AIV vaccines, and a theoretical basis for the development of safe and efficient gene delivery carriers. (C) 2020 Elsevier Inc. All rights reserved.

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