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A Lactococcus Lactis-Vectored Oral Vaccine Induces Protective Immunity Of Mice Against Enterotoxigenic Escherichia Coli Lethal Challenge

文献类型： 外文期刊

作者： Song, JJ; Zhao, LY; Song, MX

作者机构：

关键词： Oral Vaccine; Lactococcus Lactis; Enterotoxigenic Escherichia Coli; Enterotoxin; Fimbriae

期刊名称： IMMUNOLOGY LETTERS

ISSN： 0165-2478

年卷期： 2020 年 225 卷

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收录情况： JCR（2021版）

摘要： Enterotoxigenic Escherichia coli (ETEC) is a global primary pathogenic bacterium causing diarrhoea in human and a wide variety of neonatal animals. Lactococcus lactis as non-pathogenic and food-grade lactic acid bacteria has already been explored as a vector for mucosal vaccine. Here, the current study was undertaken to evaluate the live recombinant L. lactis (rL. lactis) vaccine expressing the trivalent enterotoxin protein STa-LTB-STb and the F5 fimbrial antigen (SLS-F5) with OmpH of Yersinia enterocolitica in protection against ETEC. Western blot confirmed the expression of fusion protein SLS-F5-OmpH in nisin-controlled expression (NICE) system. Mice orally immunized with rL. lactis-SLS-F5-OmpH were observed to produce high levels of mucosal SIgA and serum IgG antibodies, while also inducing increases in the production of CD4(+) and CD8(+) T cells, lymphocyte proliferation, and secretion of cytokines. Moreover, orally immunized mice produced complete protection after ETEC challenge. The above results suggested that rL. lactis-SLS-F5-OmpH has the potential as a candidate for oral vaccine against ETEC.

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