数字农科院2.0

Gmnmhc5, A Neoteric Positive Transcription Factor Of Flowering And Maturity In Soybean

文献类型： 外文期刊

作者： Wang, WT; Wang, ZL; Hou, WS; Chen, L; Jiang, BJ; Liu, W; Feng, YJ; Wu, CX

作者机构：

关键词： Glycine Max; Gmnmhc5; Flowering Regulation; Crispr-Cas9

期刊名称： PLANTS-BASEL

ISSN： 2223-7747

年卷期： 2020 年 9 卷 6 期

页码：

收录情况： JCR（2021版）

摘要： The soybean (Glycine max(L.) Merr.) is an important oil and food crop. Its growth and development is regulated by complex genetic networks, and there are still many genes with unknown functions in regulation pathways. In this study, GmNMHC5, a member of the MADS-box protein family, was found to promote flowering and maturity in the soybean. Gene expression profiling in transgenic plants confirmed that the 35S:GmNMHC5T3 generation had early flowering and precocity. We used CRISPR-Cas9 to edit GmNMHC5 and found that late flowering and maturity occurred in Gmnmhc5 lines with stable inheritance. Remarkably, in the 35S:GmNMHC5 plants, the expression of flowering inhibitors GmFT1a and GmFT4 was inhibited. In addition, overexpression of GmNMHC5 in ft-10(a late flowering Arabidopsis thaliana mutant lacking Flowering Locus T(FT) function) rescued the extremely late-flowering phenotype of the mutant A. thaliana. These results suggest that GmNMHC5 is a positive transcription factor of flowering and maturity in the soybean, which has a close relationship with FT homologs in the flowering regulation pathway. This discovery provides new ideas for the improvement of the flowering regulation network, and can also provide guidance for future breeding work.

分类号：

  • 相关文献

[1]弓形虫sod3基因缺失株的构建及其基本生物学功能研究. 赵丹钰,李婷婷,王萌,祁淑芸,梁勤立,张芝玮,朱兴全,王金磊. 2022

[2]Flowering Time Regulation By The C.onstans-Like Gene Oscol10. Tan, JJ, Wu, FQ, Wan, JM. 2017

[3]Identification Of Putative Constans-Like Genes F.rom The De Novo A ssembled Transcriptome Of Leek. Liu, C, Tang, Q, Cheng, C, Xu, Y, Yang, Z, Dai, Z, Su, J. 2018

[4]Molecular Modulation Of Root Development By Ethylene. Ma, Changle,Miao, Yuchen,Zhou, Yun,Huang, Rongfeng,Qin, Hua,Qin, Hua,Huang, Rongfeng. 2020

[5]Ah2 Encodes A Myb Domain P.rotein That Determines Hull F ate And Affects Grain Yield And Quality In Rice. Guo, Longbiao,Peng, Youlin,Qian, Qian,Gao, Zhenyu,Hu, Jiang,Zhang, Yu,Wang, Yuexing,Hu, Haitao,Yu, Xiaoqi,Rao, Yuchun,Cui, Yuanjiang,Zhang, Qiang,Zeng, Dali,Zhu, Li,Chen, Guang,Xu, Qiankun,Zhang, Guangheng,Shen, Lan,Ren, Deyong,Hu, Haitao. 2019

[6]Fon4 Prevents The Multi-Floret Spikelet I.n Rice. Qiu, Zhennan,Ren, Deyong,Zeng, Dali,Xu, Qiankun,Ren, Deyong,Zhou, Tingting,Qian, Qian,Qian, Qian,Cui, Yuanjiang,Guo, Longbiao. 2019

[7]Efficient deletion of multiple circle RNA loci by CRISPR‐Cas9 reveals Os06circ02797 as a putative sponge for OsMIR408 in rice. Jianping Zhou,Mingzhu Yuan,Yuxin Zhao,Quan Quan,Dong Yu,Han Yang,Xu Tang,Xuhui Xin,Guangze Cai,Qian Qian,Yiping Qi,Yong Zhang. 2021

[8]Research Progress And Perspective On D.rought Stress In Legumes: A Review. Nadeem, M, Li, JJ, Yahya, M, Sher, A, Ma, CX, Wang, XB, Qiu, LJ. 2019

[9]Functional Characterization Of Dense Granule P.roteins In Toxoplasma Gondii R h Strain Using Crispr-Cas9 System. Bai, MJ, Wang, JL, Elsheikha, HM, Liang, QL, Chen, K, Nie, LB, Zhu, XQ. 2018

[10]Progress And Perspective Of Biosynthetic P.latform For Higher-Order Biofuels. Su, HF, Lin, JF, Tan, FR. 2017

[11]Advances in detecting and reducing off-target effects generated by CRISPR-mediated genome editing. Chen, Wanjin,Zuo, Erwei,Hong, Shunyan,Li, Jinjing,Chen, Wanjin,Hong, Shunyan,Yang, Hui,Li, Jinjing,Hong, Shunyan,Chen, Wanjin,Li, Jinjing. 2019

[12]Functional Genetic Analysis Of The L.eucinostatin Biosynthesis Transcription Regulator L csl In Purpureocillium Lilacinum Using Crispr-Cas9 Technology. Jiao, Y, Li, Y, Li, YL, Cao, HY, Mao, ZC, Ling, J, Yang, YH, Xie, BY. 2019

[13]Characterization of GmABI3VP1 Associated with Resistance to Soybean Cyst Nematode in Glycine max. 曲硕,张淼莉,宋庚辰,胡世豪. 2025

[14]Synthesis and Degradation of the Major Allergens in Developing and Germinating Soybean Seed. Wu, Yong-Mei,Guan, Rong-Xia,Liu, Zhang-Xiong,Chang, Ru-Zhen,Qiu, Li-Juan,Wu, Yong-Mei,Li, Run-Zhi. 2012

[15]A Novel Sucrose-Regulatory MADS-Box Transcription Factor GmNMHC5 Promotes Root Development and Nodulation in Soybean (Glycine max [L.] Merr.). Liu, Wei,Han, Xiangdong,Zhan, Ge,Zhao, Zhenfang,Wu, Cunxiang,Han, Xiangdong,Zhan, Ge,Zhao, Zhenfang,Feng, Yongjun. 2015

[16]Postflowering photoperiod regulates vegetative growth and reproductive development of soybean. Han, TF,Wu, CX,Tong, Z,Mentreddy, RS,Tan, KH,Gai, JY. 2006

[17]Analyses of a Glycine max Degradome Library Identify microRNA Targets and MicroRNAs that Trigger Secondary SiRNA Biogenesis. Hu, Zheng,Jiang, Qiyan,Ni, Zhiyong,Xu, Shuo,Zhang, Hui,Chen, Rui. 2013

[18]Genetic diversity of Chinese spring soybean germplasm revealed by SSR markers. Wang, L. X.,Guan, R. X.,Li, Y. H.,Lin, F. Y.,Luan, W. J.,Li, W.,Ma, Y. S.,Liu, Z. X.,Chang, R. Z.,Qiu, L. J.. 2008

[19]Isolation and characterization of GmSTY1, a novel gene encoding a dual-specificity protein kinase in soybean (Glycine max L.). Xu, Zhao-Shi,Ma, You-Zhi,Cheng, Xian-Guo,Cao, Li-Xia,Li, Lian-Cheng,Chen, Ming. 2006

[20]Potential of marker selection to increase prediction accuracy of genomic selection in soybean (Glycine max L.). Li, Wenbin,Ma, Yansong,Liu, Zhangxiong,Guo, Yong,Qiu, Lijuan,Ma, Yansong,Luan, Xiaoyan,Reif, Jochen C.,Jiang, Yong,Wen, Zixiang,Wang, Dechun,Han, Tianfu,Wu, Cunxiang,Sun, Shi,Wei, Shuhong,Wang, Shuming,Yang, Chunming,Wang, Huicai,Yang, Chunming,Zhang, Mengchen,Lu, Weiguo,Xu, Ran,Zhou, Rong,Zhou, Xinan,Wang, Ruizhen,Sun, Zudong,Chen, Huaizhu,Zhang, Wanhai,Sun, Bincheng,Wu, Jian,Han, Dezhi,Yan, Hongrui,Hu, Guohua,Liu, Chunyan,Fu, Yashu,Chen, Weiyuan,Guo, Tai,Zhang, Lei,Yuan, Baojun.

作者其他论文 更多>>