In Vitro Activity Of Camellia Sinensis (Green Tea) Against Trophozoites And Cysts Of Acanthamoeba Castellanii
文献类型: 外文期刊
作者: Fakae, LB; Stevenson, CW; Zhu, XQ; Elsheikha, HM
作者机构:
关键词: Acanthamoeba Castellanii; Camellia Sinensis; Green Tea; Anti-Acanthamoebic Activity; Cyst; Trophozoite
期刊名称: INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
ISSN: 2211-3207
年卷期: 2020 年 13 卷
页码:
收录情况: JCR(2021版)
摘要: The effect of Camellia sinensis (green tea) on the growth of Acanthamoeba castellanii trophozoites was examined using a microplate based-Sulforhodamine B (SRB) assay. C. sinensis hot and cold brews at 75% and 100% concentrations significantly inhibited the growth of trophozoites. We also examined the structural alterations in C. sinensis-treated trophozoites using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). This analysis showed that C. sinensis compromised the cell membrane integrity and caused progressive destruction of trophozoites. C. sinensis also significantly inhibited the parasite's ability to form cysts in a dose-dependent manner and reduced the rate of excystation from cysts to trophozoites. C. sinensis exhibited low cytotoxic effects on primary corneal stromal cells. However, cytotoxicity was more pronounced in SV40-im-mortalized corneal epithelial cells. Chromatographic analysis showed that both hot and cold C. sinensis brews contained the same number and type of chemical compounds. This work demonstrated that C. sinensis has anti-acanthamoebic activity against trophozoite and cystic forms of A. castellanii. Further studies are warranted to identify the exact substances in C. sinensis that have the most potent anti-acanthamoebic effect.
分类号:
- 相关文献
作者其他论文 更多>>
-
Molecular Detection And Genotype Distribution Of Enterocytozoon Bieneusi In Farmed Silver Foxes (Vulpes Vulpes) And Arctic Foxes (Vulpes Lagopus) In Shandong Province, Eastern China
作者:Ma, YY; Zou, Y; Ma, YT; Nie, LB; Xie, SC; Cong, W; Xu, QM; Zhu, XQ
关键词:Enterocytozoon Bieneusi; Farmed Foxes; Prevalence; Genotypes; Shandong Province; China
-
Prevalence And Subtypes Of Blastocystis Sp. Infection In Zoo Animals In Three Cities In China
作者:Li, XD; Zou, Y; Pan, J; Liang, QL; Zeng, Z; Meng, YM; Wang, XL; Wang, HN; Zhu, XQ
关键词:Blastocystis; Zoo Animals; Prevalence; Subtypes; China
-
Prevalence, Risk Factors And Genotype Distribution Of Toxoplasma Gondii Dna In Soil In China
作者:Cong, W; Zhang, NZ; Hu, RS; Zou, FC; Zou, Y; Zhong, WY; Wu, JJ; Fallaize, CJ; Zhu, XQ; Elsheikha, HM
关键词:Soil Contamination; Toxoplasma Gondii; Prevalence; Genotypes; China
-
Fasciola Gigantica Excretory-Secretory Products (Fgesps) Modulate The Differentiation And Immune Functions Of Buffalo Dendritic Cells Through A Mechanism Involving Dnmt1 And Tet1
作者:Mei, XF; Shi, W; Zhao, WP; Luo, HL; Zhang, YY; Wang, YR; Sheng, ZA; Wang, DY; Zhu, XQ; Huang, WY
关键词:Fasciola Gigantica; Excretory; Secretory Products; Dendritic Cells; Differentiation; Immune Functions
-
Phylogenetic Relationships Among Toxocara Spp. And Toxascaris Sp. From Different Regions Of The World
作者:Fava, NMN; Cury, MC; Santos, HA; Takeuchi-Storm, N; Strube, C; Zhu, XQ; Taira, K; Odoevskaya, I; Panovag, O; Mateus, TL; Nejsum, P
关键词:Nematodes; Toxocara; Toxascaris; Phylogeny; Host Specificity
-
Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry-Based Metabolomics Reveals Metabolic Alterations In The Mouse Cerebellum Duringtoxoplasma Gondiiinfection
作者:Ma, J; He, JJ; Hou, JL; Zhou, CX; Elsheikha, HM; Zhu, XQ
关键词:Cerebellum; Toxoplasma Gondii; Metabolomics; Pathway Analysis; Host-Parasite Interaction
-
Characterization Of Haemonchus Contortus Excretory/Secretory Antigen (Es-15) And Its Modulatory Functions On Goat Immune Cells In Vitro
作者:Ehsan, M; Gadahi, JA; Hasan, MW; Haseeb, M; Ali, H; Yan, RF; Xu, LX; Song, XK; Zhu, XQ; Li, XR
关键词:Haemonchus contortus; 15 kDa; goat; PBMCs; host-parasite interactions; immunomodulation; cytokines