数字农科院2.0

De Novo Assembly Of The Asian Citrus Psylliddiaphorina Citri(Hemiptera: Psyllidae) Transcriptome Across Developmental Stages

文献类型： 外文期刊

作者： Yang, CX; Ou, D; Guo, W; Lu, J; Guo, CF; Qiu, BL; Pan, HP

作者机构：

关键词： Diaphorina Citri; Transcriptome Annotation; Developmental Stage; Differential Expression; Trehalase

期刊名称： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN：

年卷期： 2020 年 21 卷 14 期

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收录情况： JCR（2021版）

摘要： Asian citrus psyllidDiaphorina citriKuwayama is an important economic pest of citrus, as it transmitsCandidatusLiberibacter asiaticus, the causative agent of huanglongbing. In this study, we used RNA-seq to identify novel genes and provide the first high-resolution view of the ofD. citritranscriptome throughout development. The transcriptomes ofD. citriduring eight developmental stages, including the egg, five instars, and male and female adults were sequenced. In total, 115 million clean reads were obtained and assembled into 354,726 unigenes with an average length of 925.65 bp and an N50 length of 1733 bp. Clusters of Orthologous Groups, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes analyses were conducted to functionally annotate the genes. Differential expression analysis highlighted developmental stage-specific expression patterns. Furthermore, two trehalase genes were characterized with lower expression in adults compared to that in the other stages. The RNA interference (RNAi)-mediated suppression of the two trehalase genes resulted in significantly highD. citrimortality. This study enriched the genomic information regardingD. citri. Importantly, these data represent the most comprehensive transcriptomic resource currently available forD. citriand will facilitate functional genomics studies of this notorious pest.

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