数字农科院2.0

Coupling 16S Rdna Sequencing And Untargeted Mass Spectrometry For Milk Microbial Composition And Metabolites From Dairy Cows With Clinical And Subclinical Mastitis

文献类型： 外文期刊

作者： Wang, Y; Nan, XM; Zhao, YG; Wang, H; Wang, ML; Jiang, LS; Zhang, F; Xue, FG; Hua, DK; Li, KM; Liu, J; Yao, JH; Xiong, BH

作者机构：

关键词： Dairy Cow; Milk; Mastitis; Microbiota; Metabolomics

期刊名称： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN： 0021-8561

年卷期： 2020 年 68 卷 31 期

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： The internal environment of the cow's udder directly affects the udder health and milk quality. 16S rDNA sequencing and liquid chromatography-mass spectrometry (LC-MS) methods were used to investigate the significant differences in milk microbial diversity and metabolites among cows that are healthy (H) and those suffering from subclinical mastitis (SM) and clinical mastitis (CM). Results uncovered more than 16 and 192 differently abundant microbiota at the phylum and genus levels, respectively, and 673 different levels of metabolites enriched in 20 pathways in milk among the 3 groups. This study revealed the positive relevance between Staphylococcus and Streptococcus and ceramide in milk from CM cows. Similarly, Acinetobacter and Corynebacterium were positively associated with testosterone glucuronide and 5-methyl-tetrahydrofolate, in milk from SM cows. On the basis of the combined analysis of microbiome and metabolome, this study indicated that, apart from the exogenous pathogens, some beneficial symbiotic bacteria, such as Dietzia, Aeromicrobium, Alistipes, and Sphingobacterium, rarely reported in milk have been found to be significantly reduced during mastitis.

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