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EXPRESSION OF PHEROMONE BIOSYNTHESIS ACTIVATING NEUROPEPTIDE AND ITS RECEPTOR (PBANR) mRNA IN ADULT FEMALE Spodoptera exigua (LEPIDOPTERA: NOCTUIDAE)

文献类型： 外文期刊

作者： Luo, Lizhi;Jiang, Xingfu;Zhang, Lei;Niu, Changying

作者机构：

关键词： beet armyworm;sex pheromone;PBAN;PBANR G protein–coupled receptor

期刊名称： ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY

ISSN：

年卷期：

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收录情况： SCI

摘要： The full-length cDNA of pheromone biosynthesis activating neuropeptide receptor (PBANR) was cloned from the beet armyworm, Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae); it included an open reading frame of 1,053 bp encoding 350 amino acids. ThePBANR of S. exigua (SePBANR) was structurally characteristic of G protein–coupled receptor and its amino acid sequence shared 98% identity with the PBANR of Spodoptera littoralis. Both pheromone biosynthesis activating neuropeptide (PBAN) and PBANR mRNA abundance were measured in the brain-subesophageal ganglion complex, pheromone gland, ventral nerve cord, and ovary of S. exigua female moths by real-time RT-PCR. The abundance of PBAN mRNA in brain-subesophageal ganglion complex and PBANR mRNA in pheromone gland was significantly greater compared to other tissues, suggesting that the ligand-receptor relationship of PBAN and PBANR exists quantitatively in S. exigua.Both PBAN and PBANR expression displayed a remarkable diurnal rhythm, for they were low and stable during the photophase

分类号： Q966

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