A xylanase gene directly cloned from the genomic DNA of alkaline wastewater sludge showing application potential in the paper industry
文献类型: 外文期刊
作者: Zhao, Yanyu;Luo, Huiying;Meng, Kun;Shi, Pengjun;Wang, Guozeng;Yuan, Tiezheng;Yao, Bin;Zhao, Yanyu;Yang, Peilong
作者机构:
关键词: Alkaline wastewater sludge;Direct cloning and heteroexpression;Hardwood xylan;Xylanase
期刊名称: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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年卷期:
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收录情况: SCI
摘要: A xylanase gene, aws-2x, was directly cloned from the genomic DNA of the alkaline wastewater sludge using degenerated PCR and modified TAIL-PCR. The deduced amino acid sequence of AWS-2x shared the highest identity (60%) with the xylanase from Chryseobacterium gleum belonging to the glycosyl hydrolase GH family 10. Recombinant AWS-2x was expressed in Escherichia coli BL21 (DE3) and purified to electrophoretic homogeneity. The enzyme showed maximal activity at pH 7.5 and 55 °C, maintained more than 50% of maximal activity when assayed at pH 9.0, and was stable over a wide pH range from 4.0 to 11.0. The specific activity of AWS-2x towards hardwood xylan (beechwood and birchwood xylan) was significantly higher than that to cereal xylan (oat spelt xylan and wheat arabinoxylan). These properties make AWS-2x a potential candidate for application in the pulp and paper industry.
分类号: Q5
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