数字农科院2.0

Evaluation of molecular methods for detection of Borrelia burgdorferi senso lato in ticks

文献类型： 外文期刊

作者： Yang, Jifei;Liu, Zhijie;Guan, Guiquan;Che, Rongxuan;Niu, Qingli;Li, Youquan;Liu, Junlong;Ma, Miling;Ren, Qiaoyun;Liu, Aihong;Luo, Jianxun;Yin, Hong

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关键词： Borrelia burgdorferi sensu lato;PCR;Prevalence;Tick

期刊名称： DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE

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收录情况： SCI

摘要： Borrelia burgdorferi sensu lato (s. l.), the agent of Lyme disease, is distributed widely worldwide. A large number of polymerase chain reaction (PCR) methods have been developed and used for detection of . B. burgdorferi s. l. However, there is a lack of a reference standard because of the genetic diversity of the . B. burgdorferi s. l. complex. In this study, 4 PCR methods, based on the . OspA, . flagellin, . rrs, and . P66 genes, for detection of . B. burgdorferi s. l. were evaluated by detection of genomic DNA from 3 reference genospecies and tick samples. The sensitivity of the PCR methods was analyzed using serially diluted gDNA from . B. afzelii (Bo23), . B. burgdorferi sensu stricto (B31), and . B. garinii (PBi). The performance of the PCRs was evaluated by detection of the gDNA of 543 ticks. The results showed that the PCRs targeting the . OspA gene, . fla gene, . rrs gene, and . P66 gene detected 37 (6.8%), 74 (13.6%), 16 (2.9%), and 14 (2.6%) tick samples, respectively. The PCR targeting the . fla gene was the most sensitive method for the detection of . B. burgdorferi s. l.

分类号： R51

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