数字农科院2.0

A recently identified ovine Babesia in China: Serology and sero-epidemiology

文献类型： 外文期刊

作者： Guan, Guiquan;Ma, Miling;Liu, Aihong;Ren, Qiaoyun;Wang, Jinming;Yang, Jifei;Li, Anyan;Liu, Zhijie;Du, Pengfei;Li, Youquan;Liu, Qing;Zhu, Hai;Yin, Hong;Luo, Jianxun

作者机构：

关键词： Babesia sp. in Xinjiang;Merozoite antigens;ELISA;Western blotting;Sero-epidemiology

期刊名称： PARASITOLOGY INTERNATIONAL

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年卷期：

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收录情况： SCI

摘要： Babesia sp. in Xinjiang, transmitted by Hyalomma, is a large Babesia that is infective for small ruminants, but it has almost no pathogenicity in healthy sheep. On the basis of the sequences of the 18S rRNA and internal transcribed spacer (ITS) genes, morphological characteristics, vector tick species and pathogenicity it was identified recently as a novel Babesia species. In the present study, an enzyme-linked immunosorbent assay (ELISA) was developed using soluble merozoite antigens of Babesia sp. in Xinjiang (BXJMA) derived from in vitro culture. When the positive threshold was chosen as 24.65% of the specific mean antibody rate, the specificity and sensitivity were both 97.3%. There was no cross-reaction between BXJMA and positive sera from sheep infected with other Chinese ovine piroplasms or Anaplasma ovis in the ELISA and western blotting. Specific antibodies against Babesia sp. in Xinjiang could be detected 2 weeks post infection and a high level of antibodies persisted for more than 12 weeks in experimentally infected sheep. The ELISA was tested on 3857 sera collected from small ruminants in 50 prefectures of 22 provinces to evaluate the sero-epidemiology of Babesia sp. in Xinjiang infection, and the average positive rate was 31.66%. These data provide that the developed ELISA is a powerful tool for the sero-diagnosis of Babesia sp. in Xinjiang and confirm that it is a novel species.

分类号： Q18

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