数字农科院2.0

Molecular cloning and expression analysis of a putative sesquiterpene synthase gene from tea plant (Camellia sinensis)

文献类型： 外文期刊

作者： Fu, Jian-yu

作者机构：

关键词： Tea plant;Camellia sinensis;Cstps1;Terpene synthase;Gene

期刊名称： ACTA PHYSIOLOGIAE PLANTARUM

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A putative gene encoding germacrene D synthase (GenBank Accession No. JQ247185) from tea plant (Camellia sinensis) was isolated by rapid amplification of cDNA ends for the first time, which was designated as tea plant gene terpene synthase 1 (Cstps1).This full-length cDNA was 2,090 nucleotides, and the open reading frame was 1,704 bp encoding polypeptides of 568 amino acids. The deduced amino acid sequence contained two characteristic conserved motifs of RRx8W and DDxxD, which showed high homology with sesquiterpene synthases of angiosperms, and the highest level of similarity was 73 % to germacrene D synthase from Actinidia deliciosa (Accession NO. AAX16121.1). Phylogenetic analysis indicated that Cstps1 belonged to the terpene synthase-a subfamily of plant, which was the largest clade of terpene synthases and mainly composed of sesquiterpene synthases. The Cstps1 expression was detected in different organs, with high expression levels in leaf and flower, weak expression in stem and root. Real-time qRT-PCR indicated that Cstps1 could be induced by insect damage, this result suggested Cstps1 might have potential ecological function during tea plant defense response.

分类号： Q94

  • 相关文献

[1]Plant-derived monoterpene S-linalool and β-ocimene generated by CsLIS and CsOCS-SCZ are key chemical cues for attracting parasitoid wasps for suppressing Ectropis obliqua infestation in Camellia sinensis L. Liu, Guanhua,Wang, Qian,Chen, Hui,Wang, Yuxi,Zhou, Xiaogui,Bao, Demeng,Wang, Nuo,Sun, Juan,Huang, Fuyin,Yang, Mei,Zhang, Han,Yan, Peng,Li, Xin,Shi, Jiang,Fu, Jianyu. 2024

[2]Genotypic variation of beta-carotene and lutein contents in tea germplasms, Camellia sinensis (L.) O. Kuntze. Wang, Xin-Chao,Chen, Liang,Ma, Chun-Lei,Yao, Ming-Zhe,Yang, Ya-Jun. 2010

[3]Identification and Evaluation of Reliable Reference Genes for Quantitative Real-Time PCR Analysis in Tea Plant (Camellia sinensis (L.) O. Kuntze). Hao, Xinyuan,Yang, Yajun,Xiao, Bin,Hao, Xinyuan,Horvath, David P.,Chao, Wun S.,Hao, Xinyuan,Yang, Yajun,Wang, Xinchao,Hao, Xinyuan,Yang, Yajun,Wang, Xinchao. 2014

[4]Biochemical and transcriptomic analyses reveal different metabolite biosynthesis profiles among three color and developmental stages in 'Anji Baicha' (Camellia sinensis). Li, Chun-Fang,Xu, Yan-Xia,Ma, Jian-Qiang,Jin, Ji-Qiang,Huang, Dan-Juan,Yao, Ming-Zhe,Ma, Chun-Lei,Chen, Liang,Li, Chun-Fang. 2016

[5]Global transcriptome and gene regulation network for secondary metabolite biosynthesis of tea plant (Camellia sinensis). Li, Chun-Fang,Wang, Xin-Chao,Yao, Ming-Zhe,Chen, Liang,Yang, Ya-Jun,Zhu, Yan,Yu, Yao,Zhao, Qiong-Yi,Li, Xuan,Wang, Sheng-Jun,Luo, Da. 2015

[6]Recent Advances in the Specialized Metabolites Mediating Resistance to Insect Pests and Pathogens in Tea Plants (Camellia sinensis). Zhang J.,Yu Y.,Qian X.,Zhang X.,Li X.,Sun X.. 2024

[7]猪瘟病毒结构蛋白E^ms在E．coli中的高效表达及纯化. 尹双辉,刘湘涛,韩雪清,尚佑军,孙士琪. 2006

[8]Microbial-type terpene synthase genes occur widely in nonseed land plants, but not in seed plants. Jia, Qidong,Chen, Feng,Li, Guanglin,Fu, Jianyu,Chen, Xinlu,Xiong, Wangdan,Chen, Feng,Li, Guanglin,Kollner, Tobias G.,Gershenzon, Jonathan,Fu, Jianyu,Crandall-Stotler, Barbara J.,Bowman, John L.,Weston, David J.,Zhang, Yong,Chen, Li,Xie, Yinlong,Wong, Gane Ka-Shu,Li, Fay-Wei,Rothfels, Carl J.,Larsson, Anders,Graham, Sean W.,Stevenson, Dennis W.,Wong, Gane Ka-Shu,Wong, Gane Ka-Shu.

[9]Tissue-Specific Expression of the Terpene Synthase Family Genes in Rosa chinensis and Effect of Abiotic Stress Conditions. Yuhang Yan,Mouliang Li,Xiaoni Zhang,Weilong Kong,Mohammed Bendahmane,Manzhu Bao,Xiaopeng Fu. 2022

[10]Terpene Synthases in Rice Pan-Genome and Their Responses to Chilo suppressalis Larvae Infesting. Yang Sun, Pei-tao Zhang,Dou-rong Kou, Yang-chun Han, Ji-chao Fang, Jiang-ping Ni, Bin Jiang, Xu Wang, Yong-jun Zhang, Wei Wang, Xiang-dong Kong. 2022

[11]The genome assembly of Chimonanthus praecox var. concolor and comparative genomic analysis highlight the genetic basis underlying conserved and variable floral traits of wintersweet. Yifan Jiang,Fei Chen,Aixia Song,Yiyong Zhao,Xinlu Chen,Yuan Gao,Guo Wei,Wanbo Zhang,Yaqin Guan,Jianyu Fu,Junhao Chen,Wenlei Guo,Zhengjia Wang,Tobias G. Köllner,Zhineng Li,Ning Liu,Shunzhao Sui,Liangsheng Zhang,Feng Chen. 2023

[12]Analysis of Fitness Predominance for Gaoyou Duck's Double Yolk Egg. Zhang, Tangjie,Chang, Hong,Li, Hui-Fang,Chen, Kuanwei,Zhao, Yonggao,Xuec, Minkai,Zhang, Shengfu. 2011

[13]Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder. Suo, Huayi,Xie, Jie,Song, Jia-Le,Song, Jia-Le,Yi, Ruokun,Zhou, Yalin,Zhu, Kai,Zhao, Xin,Liu, Zhenhu,Zhao, Xin. 2015

[14]Genomic analysis of an effective lentiviral vaccine-attenuated equine infectious anemia virus vaccine EIAV(FDDV13). Qi, Xu,Su, Zhiqiang,Wang, Xuefeng,Wang, Shuai,Lin, Yuezhi,Jiang, Chenggang,Ma, Jian,Zhao, Liping,Lv, Xiaoling,Shen, Rongxian,Kong, Xiangang,Zhou, Jianhua,Wang, Xuefeng,Wang, Fenglong,Ma, Jian. 2010

[15]Isolation and Expression Analysis of Sucrose Synthase Gene (ScSuSy4) from Sugarcane. Gui, Yi-Yun,Qin, Cui-Xian,Wang, Miao,Huang, Dong-Liang,Chen, Zhong-Liang,Gui, Yi-Yun,Qin, Cui-Xian,Wang, Miao,Huang, Dong-Liang,Chen, Zhong-Liang,Gui, Yi-Yun,Qin, Cui-Xian,Wang, Miao,Huang, Dong-Liang,Chen, Zhong-Liang,Gui, Yi-Yun,Qin, Cui-Xian,Wang, Miao,Huang, Dong-Liang,Liao, Qing,Li, Yang-Rui. 2016

[16]Stage-Specific developmental gene expression of goat preimplantation embryos produced in vitro. Li, Yong-Jun,Zhang, Li,Li, Yong-Jun,Ao, Hong,Sun, Gui-Jin,Sun, Gui-Jin.

[17]Peptidyl-prolyl isomerization targets rice Aux/IAAs for proteasomal degradation during auxin signalling. Jing, Hongwei,Yang, Xiaolu,Zhang, Jian,Zheng, Huakun,Nian, Jinqiang,Feng, Jian,Li, Jiayang,Zuo, Jianru,Jing, Hongwei,Yang, Xiaolu,Zhang, Jian,Zheng, Huakun,Nian, Jinqiang,Feng, Jian,Li, Jiayang,Zuo, Jianru,Jing, Hongwei,Yang, Xiaolu,Zheng, Huakun,Liu, Xuehui,Dong, Guojun,Qian, Qian,Xia, Bin.

[18]The degradation effects of a Pseudomonas hydrolase OPHC2 to organophosphorus insecticides. Wang, Jin-Fang,Gao, Ming-Hui,Pan, Can-Ping,Wu, Ning-Feng.

[19]Spread of recombinant Autographa californica nucleopolyhedrovirus in various tissues of silkworm Bombyx mori determined by real-time PCR. Zhang, Yi,Tian, Baozhong,Guo, Tingqing,Wang, Jianyang,Wei, Zhenguo,Lu, Changde,Zhang, Yi,Xia, Huanzhang,Tian, Baozhong,Wei, Zhenguo,Wang, Shengpeng.

[20]The phylogeographic system survey of native sheep breeds in the eastern and southern Central Asia. Sun, W.,Ma, Y. H.,Guan, W. J.,Sun, W.,Chang, H.,Musa, H. H.,Yang, Z. P.,Tsunoda, K.,Musa, H. H..

作者其他论文 更多>>