Scaling up of high-speed countercurrent chromatographic apparatus with three columns connected in series for rapid preparation of (-)-epicatechin
文献类型： 外文期刊
作者： Du, Qingbao;Yin, Junfeng;Xu, Yongquan;Du, Wenkai;Li, Bo;Du, Qizhen;Jiang, Heyuan;Yin, Junfeng;Xu, Yongquan
作者机构：
关键词： (-)-Epicatechin;(-)-Epicatechin gallate;Flash countercurrent chromatography;High-speed countercurrent chromatography;Scale-up
期刊名称： JOURNAL OF CHROMATOGRAPHY A
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： In the present study, compact high-speed countercurrent chromatographic apparatus was constructed with three columns connected in series. Two sets of columns were prepared from 10. mm and 12. mm I.D. tubing to form 12. L and 15. L capacities, respectively. Performance of these columns was compared for the separation of (-)-epicatechin gallate (ECG) from a tea extract by flash countercurrent chromatography (FCCC). In each separation, 200. g of the tea extract in 1600. mL of mobile phase was loaded onto the column. The 12. L column gave 7.5. L (35. g of ECG) and the 15. L column gave 9. L (40. g of ECG) of ECG solution without impurities. The ECG solution was directly hydrolyzed by tannase into (-)-epicatechin. The hydrolysate was purified by flash chromatography on AB-8 macroporous resin to give 52. g of EC (purity 99.1%). This scaled up apparatus could be used for the industrial separation of natural products.
分类号： O65
- 相关文献
作者其他论文 更多>>
-
Nitrogen Dynamics and Environmental Sustainability in Rice-Crab Co-Culture System: Optimal Fertilization for Sustainable Productivity
作者：Li, Hao;Wu, Shuxia;Xu, Yang;Li, Weijing;Zhang, Xiushuang;Ma, Siqi;Sun, Wentao;Li, Bo;Fan, Bingqian;Lei, Qiuliang;Liu, Hongbin
关键词：field water;rice-crab co-culture;nitrogen application rate;nitrogen transformation;environmental sustainability
-
Colorimetric Detection of H2O2 and Glucose Based on Novel Zn3(OH)2V2O72H2O Nanozymes with Superior Peroxidase-Like Properties
作者：Wang, Yingwen;Chi, Jingtian;Bi, Fan;Du, Qingbao;Zhou, Shanding;Wen, Siyu;Xiang, Zhenbo B.;Ju, Peng
关键词：Zn-3(OH)(2)V2O72H(2)O;2H(2)O nanobelts were developed as novel peroxidase mimics to establish a rapid and ultrasensitive colori-metric sensing platform for detecting H2O2 and glucose
-
The probing for nanoparticles in soybean protein isolate solutions
作者：Pan, Weichun;Wu, Wenhan;Ma, Shuang;Hu, Xinyu;Zhang, Bo;Yin, Junfeng;Niu, Fuge
关键词：Soybean protein isolate (SPI);Nanoparticles (NPs);Interactions/bonds;Particle size;zeta-Potential;Geometrical shape
-
Screening and Validation of Negative Regulators of the Type I Interferon Pathway
作者：Pang, Zhaojun;Yang, Han;Li, Bo;Liu, Yifu;Zhao, Zhenxiang;Zhu, Zixiang;Peng, Shouchun;Mu, Xin;Yu, Hongjian
关键词：CRISPR KO library;interferon, ITPKA;MAVS;negative regulators;PCGF3, UCK2
-
How can China curb biological invasions to meet Kunming-Montreal Target 6?
作者：Ju, Rui-Ting;Gui, Xiting;Measey, John;He, Qiang;Xian, Xiaoqing;Liu, Jianguo;Sutherland, William J.;Li, Bo;Wu, Jihua
关键词：Kunming-Montreal Target; Biological Invasions; China
-
Physiological and biochemical variations in different pepper (Capsicum annuum var. conoides) varieties under salt stress
作者：Li, Xinru;Zhang, Yamin;Zhang, Doudou;Liu, Junhao;Xu, Nan;Zhong, Yike;Han, Xu;Chen, Zhuona;Hua, Yingpeng;Lu, Nan;Li, Bo;Wang, Yinggang;Li, Wenyue;Shang, Haihong
关键词：Pod pepper;Salt stress;Physiological profiling;Transcriptomics;Metabolomics
-
Multiscale Elucidation of Taste Interactions in Osmanthus Green Tea: Bitter Masking via EGCG and Phenylethanoid Glycoside Interplay
作者：Huang, Qinle;Wang, Zhangtie;Xu, Yongquan;Gao, Liang;Hu, Linfu;Ji, Shengyang;Lu, Baiyi
关键词：summer-autumngreen tea;Osmanthus fragrans;EGCG;catechins;phenylethanoid glycosides;bitterness masking;molecular dynamics