数字农科院2.0

Fine mapping and candidate gene analysis of LM3, a novel lesion mimic gene in rice

文献类型： 外文期刊

作者： Shi, Chunhai;Zeng, Yuxiang;Ma, Liangyong;Ji, Zhijuan;Wen, Zhihua;Li, Ximing;Yang, Changdeng

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关键词： lesion mimic mutant;LM3;rice (Oryza sativa L.);uroporphyrinogen decarboxylase

期刊名称： BIOLOGIA

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收录情况： SCI

摘要： A rice lesion mimic mutant, lm3, was obtained by the mutagenesis of an indica cultivar, 93-11, using γ-ray radiation. Brownish lesions appeared on the leaves of lm3 at the young seedling stage and persisted until the ripening stage. The lm3 mutant was characterised by a shorter plant height and delayed heading compared with the wild-type 93-11. A genetic analysis indicated that the lesion mimic phenotype was controlled by a single recessive gene. Using simple sequence repeat (SSR) markers, the target gene LM3 was first located between marker RM5748 and RM14906 on chromosome 3. We then developed Insertion-Deletion (InDel) markers to fine-map LM3, and the locus was localised to a 29 kb region defined by two InDel markers, In12571 and In12600. Five ORFs were predicted in the candidate region, and DNA sequencing detected a single-nucleotide polymorphism (SNP) in the coding region of LOC Os03g21900. The SNP in the fourth exon (C in 93-11; T in lm3) of LOC_Os03g21900 results in the substitution of a proline (P) with a serine (S) at the 140~(th) amino acid of the deduced uroporphyrinogen decarboxylase protein. We did not detect polymorphisms in the other predicted ORF regions between lm3 and 93-11. These results suggest that LOC_Os03g21900 is the most likely candidate gene for LM3.

分类号： Q

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