数字农科院2.0

BMO-MIR-9A DOWN REGULATES THE EXPRESSION OF BM-ASE GENE IN VITRO

文献类型： 外文期刊

作者： Song, Fei;Huang, Yong;Wang, Xin;Tang, Shunming;Shen, Xingjia;Shen, Xingjia;Huang, Yong

作者机构：

关键词： Bombyx mori;MicroRNA;Bmo-miR-9a;Bm-ase;Post-transcriptional regulation

期刊名称： RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY

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年卷期：

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收录情况： SCI

摘要： MicroRNAs (miRNAs) are a class of non-protein coding small RNAs of 18-24 nucleotides in length that regulate expression of genes at post-transcriptional levels and play multiple roles in biological processes. Bm-ase plays an important role in the course of nerve development of the silkworm, Bombyx mori. Bmo-miR-9a is a conservative miRNA. By using target prediction software R.NA22 and RNAhybrid, we found a target site of Bmo-miR-9a in the 3'UTR of Bm-ase gene. To verify the regulation function of Bmo-miR-9a on the expression of Bm-ase gene, a Bmo-miR-9a over-expressing vector and Bm-ase 3'UTR fused firefly luciferase gene reporter plasmid were constructed, respectively. Then they were used to co-transfect the BmN cells. The result showed that luciferase activity in the co-transfected cells was suppressed compared with the control. A similar result was obtained when BmN cells were co-transfected with artificial synthetic Bmo-miR-9a mimics and Bm-ase 3'UTR fused luciferase reporter plasmid. These results suggest that Bmo-miR-9a can down regulate the expression of Bm-ase gene.

分类号： Q50

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