数字农科院2.0

Characterization of cry9Da4, cry9Eb2, and cry9Ee1 genes from Bacillus thuringiensis strain T03B001

文献类型： 外文期刊

作者： Su, Huiqin;Shu, Changlong;Su, Huiqin;Zhang, Jie;He, Kanglai;Huang, Dafang;Song, Fuping

作者机构：

关键词： Bacillus thuringiensis;Cry1Ac resistance;Cry9Eb2 and Cry9Ee1;High-resolution melting analysis

期刊名称： APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

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年卷期：

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收录情况： SCI

摘要： Three cry9 genes, cry9Da4, cry9Eb2, and cry9Ee1, were cloned from Bacillus thuringiensis strain T03B001 using a high-resolution melting analysis method. All three cry9 genes were overexpressed in Escherichia coli Rosetta (DE3), and the expressed products Cry9Eb2 and Cry9Ee1 were shown to be toxic to Plutella xylostella and Ostrinia furnacalis, but not to Helicoverpa armigera or Colaphellus bowringi. The bioassay of Cry9Eb2 and Cry9Ee1 against Cry1Ac-resistant P. xylostella strains indicated that both novel Cry9 toxins exhibited no cross-resistance with Cry1Ac. Cry9Eb2 and Cry9Ee1 can be applied not only for P. xylostella and O. furnacalis control, but also for the Cry1Ac-resistance management of pests.

分类号： Q939.9`Q81

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