数字农科院2.0

CLONING AND EXPRESSION ANALYSIS OF G-PROTEIN G alpha q SUBUNIT AND G beta 1 SUBUNIT FROM Bemisia tabaci GENNADIUS (HOMOPTERA: ALEYRODIDAE)

文献类型： 外文期刊

作者： Tu, Hongtao;Qin, Yuchuan;Tu, Hongtao

作者机构：

关键词： Bemisia tabaci;Gq subunit;G1 subunit;gene cloning;expression pattern

期刊名称： ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The heterotrimeric G proteins play an essential role in a wide variety of signal transduction pathways, mediating the process of chemical signals from the environment in all higher eukaryotic organisms. In this article, two G-protein subunit genes encoding Gq and G1 were cloned from Bemisia tabaci Gennadius. The full-length cDNA sequence of BtGq consisted of 2,336 bp with an ORF of 1,062 bp encoding 353 amino acids and BtG1 had a full length of 1,942 bp with an ORF of 1,023 nucleotides encoding 340 amino acids. The amino acid sequences of BtGq and BtG1 from B. tabaci B biotype were identical to those from the Q biotype. Phylogenetic analysis identified G protein and subunit families from insects based on their amino acid sequences. The expression patterns of BtGq and BtG1 at different development stages and in different body regions were analyzed by real-time quantitative PCR and Western blot. The results show that BtGq and BtG1 are neither developmental stage-specific nor tissue-specific. The transcript levels of BtGq in the B biotype are similar to that in the Q biotype, the transcript levels of BtG1 at egg, first instar and pupae in B biotype were significantly higher than that in Q biotype. The transcript levels of BtGq and BtG1 in the head were significantly higher than those in thorax and abdomen indicating that they are involved in nervous system and sensory functions.

分类号： Q966

  • 相关文献

[1]Cloning and characterization of ADP-glucose pyrophosphorylase small subunit gene in Cyperus esculentus (yellow nutsedge). Cheng, C.,Hu, J.,Zhi, Y.,Su, J. J.,Huang, B. Q.,Zhang, X. K.. 2015

[2]Gene cloning, expression and vaccine testing of Schistosoma japonicum SjFABP. Liu, JM,Cai, XZ,Lin, JJ,Fu, ZQ,Yang, GZ,Shi, FH,Cai, YM,Shen, W,Taylor, MG,Wu, XF. 2004

[3]Cloning and characterization of the nicotianamine synthase gene in Eruca vesicaria subsp sativa. Huang, B. L.,Cheng, C.,Zhang, G. Y.,Su, J. J.,Zhi, Y.,Xu, S. S.,Cai, D. T.,Huang, B. Q.,Zhang, X. K.. 2015

[4]BioVector, a flexible system for gene specific-expression in plants. Wang, Xu,Fan, Chengming,Zhang, Xiaomei,Zhu, Jinlong,Fu, Yong-Fu. 2013

[5]Cloning and Expression Analysis of Two MADS-Box Genes from Strawberry Fruit. Zhou, H. C.,Kang, Z. R.,Zhao, X.,Li, G.,Kang, Z. R.,Li, L..

[6]Cloning and characterization of the dehydration-responsive element-binding protein 2A gene in Eruca vesicaria subsp sativa. Huang, B. L.,Li, Y. Y.,Li, D. Y.,Ma, M. Y.,Cai, D. T.,Wu, W. H.,Huang, B. Q.,Zhang, X. K.. 2016

[7]Cloning and sequence analysis of Alcaligenes faecalis nifHDK gene cluster. Zhang, HY,Lin, M,Xiao, FH,Zhu, HS,Fang, XJ,You, CG,Zhu, YX.

[8]Isolation and characterization of a gene from Medicago sativa L., encoding a bZIP transcription factor. Yang, Qingchuan,Kang, Junmei,Zhang, Tiejun,Sun, Yan,Fang, Feng.

[9]Identification and characterization of a gene encoding a putative lysophosphatidyl acyltransferase from Arachis hypogaea. Chen, Si-Long,Huang, Jia-Quan,Lei, Yong,Zhang, Yue-Ting,Ren, Xiao-Ping,Chen, Yu-Ning,Jiang, Hui-Fang,Yan, Li-Ying,Liao, Bo-Shou,Chen, Si-Long,Li, Yu-Rong,Chen, Si-Long.

[10]Alternative splicing and tissue expression of CIB4 gene in sheep testis. Yu, Yan,Wei, Caihong,Lu, Guobin,Zhang, Ju,Ren, Hangxing,Sheng, Xihui,Du, Lixin,Yu, Yan,Guan, Qun,Zhang, Qiyao,Zhang, Yuan,Wang, Chuduan,Song, Xuemei,Jin, Mei,Li, Shangang. 2010

[11]Cloning and expression of gynecophoral canal protein gene of Schistosoma japonicum (Chinese strain). Jin, YM,Lin, JJ,Feng, XG,Zhang, L,Wu, XF,Zhou, YC,Cai, YM. 2002

[12]Cloning of Schistosoma japonicum Chinese strain fatty acid binding protein (Sj-FABPc) gene and its overproduction in Escherichia coli. Cai, XZ,Tian, E,Yang, GZ,Shi, FH,Shen, W,Cai, YM,Wu, XF. 1998

[13]Cloning of Schistosoma japonicum Chinese strain TPI gene and characterization of its expression product in Escherichia coli. Cai, XZ,Lin, JJ,Yang, GZ,Shi, FH,Shen, W,Cai, YM,Wu, XF. 1998

[14]Cloning and expression of actin gene of Schistosoma japonicum Chinese strain. Zhu, JG,Lin, JJ,Feng, XG,Wu, XF,Zhou, YC,Cai, YM. 2000

[15]The Research on Actinidia eriantha Benth. in South China. Wu, Y. J.,Xie, M.,Zhang, Q. C.,Zhang, H. Q.,Fang, J. B.. 2011

[16]VfCPK1, a gene encoding calcium-dependent protein kinase from Vicia faba, is induced by drought and abscisic acid. Liu, Guanshan,Chen, Jia,Wang, Xuechen. 2006

[17]Purification, gene cloning and characterization of an acidic beta-1,4-glucanase from Phialophora sp G5 with potential applications in the brewing and feed industries. Zhao, Junqi,Shi, Pengjun,Yuan, Tiezheng,Huang, Huoqing,Li, Zhongyuan,Meng, Kun,Yang, Peilong,Yao, Bin. 2012

[18]High-level expression and biochemical characterization of a novel cold-active lipase from Rhizomucor endophyticus. Duan, Xiaojie,Liu, Yu,Jiang, Zhengqiang,Yang, Shaoqing,Zheng, Mingming.

[19]Molecular Cloning, Sequence Analysis, and Expression of the Polygalacturonase-inhibiting Protein (PGIP) Gene in Mulberry. Hu, Dongqing,Dai, Ruiqiang,Wang, Yuhua,Zhang, Yinghua,Liu, Zhaoyue,Zhao, Weiguo,Hu, Dongqing,Dai, Ruiqiang,Wang, Yuhua,Zhang, Yinghua,Liu, Zhaoyue,Fang, Rongjun,Zhao, Weiguo,Li, Long,Li, Liu,Fang, Rongjun,Lin, Qiang.

[20]Mulberry (Morus L.) methionine sulfoxide reductase gene cloning, sequence analysis, and expression in plant development and stress response. Tong, Wei,Zhang, Yinghua,Wang, Heng,Li, Feng,Liu, Zhaoyue,Wang, Yuhua,Fang, Rongjun,Zhao, Weiguo,Li, Long,Wang, Yuhua,Zhao, Weiguo,Li, Long,Fang, Rongjun.

作者其他论文 更多>>