数字农科院2.0

A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C

文献类型： 外文期刊

作者： Ding, Yao-zhong;Zhou, Jian-hua;Ma, Li-na;Qi, Yan-ni;Zhang, Jie;Zhang, Yong-guang;Ding, Yao-zhong;Zhang, Jie;Zhang, Yong-guang;Wei, Gang

作者机构：

关键词： foot-and-mouth disease;foot-and-mouth disease virus serotype C;reverse transcription loop-mediated isothermal amplification;reverse transcription-PCR;sensitivity

期刊名称： JOURNAL OF VETERINARY SCIENCE

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年卷期：

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收录情况： SCI

摘要： A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription-PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swine vesicular disease virus (SVDV) indicated that FMDV C RT-LAMP may be an exciting novel method for detecting FMDV C.

分类号： S8

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