数字农科院2.0

Near-infrared reflectance spectroscopy (NIRS) for rapid determination of ginsenoside Rg1 and Re in Chinese patent medicine Naosaitong pill

文献类型： 外文期刊

作者： Zhang, Wei;Qu, Zhengyi;Wang, Yingping;Yao, Chunlin;Bai, Xueyuan;Bian, Shuai;Zhao, Bing

作者机构：

关键词： Near-infrared spectroscopy;Partial least square;Naosaitong pill

期刊名称： SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

ISSN：

年卷期：

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收录情况： SCI

摘要： Ginsenosides in plant samples have been extensively studied because protopanaxadiol saponins are ubiquitous in Chinese patent medicines, in which they can be used in promoting human health as the main active ingredients. A method for rapid determination of two ginsenosides (Rgi and Re) in Naosaitong (NST) samples using near-infrared reflectance spectroscopy (NIRS) is studied to determine the contents of ginsenoside Rgl and Re in this work. Partial least square (PLS) regression was used for building the calibration models, and the effects of spectral preprocessing and variable selection on the models are investigated for optimization of the models. A total of 93 samples were scanned by NIRS, and also by high performance liquid chromatography coupled to a diode array detector to determine the contents of ginsenoside Rgi and Re. The calibration models for Rgl and Re had high values of the coefficient of determination (R-2) (0.9766 and 0.9764) and low root mean square error of cross validation (RMSECV) (0.0136 and 0.0104), and the values of the standard error of prediction set (SEP) are 0.00764 and 0.0103, which indicate a good correlation between reference values and NIRS predicted values. The overall results show that NIRS could be applied for the rapid determination of the contents of ginsenosides in Ginseng byproducts for pharmaceuticals that develop high-quality Chinese patent medicines. (C) 2014 Elsevier B.V. All rights reserved.

分类号： O657.3

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