数字农科院2.0

Homo logs to Cry toxin receptor genes in a de novo transcriptome and their altered expression in resistant Spodoptera litura larvae

文献类型： 外文期刊

作者： Gong, Liang;Wang, Huidong;Qi, Jiangwei;Hu, Meiying;Han, Lanzhi;Jurat-Fuentes, Juan Luis

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关键词： Spodoptera litura;Bacillus thuringiensis;Transcriptome;Cry1 Ca receptor;Resistance

期刊名称： JOURNAL OF INVERTEBRATE PATHOLOGY

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收录情况： SCI

摘要： Insect resistance threatens sustainability of insecticides based on Cry proteins from the bacterium Bacillus thuringiensis (Bt). Since high levels of resistance to Cry proteins involve alterations in Cry-binding midgut receptors, their identification is needed to develop resistance management strategies. Through Illumina sequencing we generated a transcriptome containing 16,161 annotated unigenes for the Oriental leafworm (Spodoptera litura). Transcriptome mining identified 6 contigs with identity to reported lepidopteran Cry toxin receptors. Using PCR we confirmed their expression during the larval stage and compared their quantitative expression in larvae from susceptible and a field-derived Cry1 Ca resistant strain of S. litura. Among reduced transcript levels detected for most tested contigs in the Cry1 Ca-resistant S. litura larvae, the most dramatic reduction (up to 99%) was detected for alkaline phosphatase contigs. This study significantly expands S. litura transcriptomic resources and provides preliminary identification of putative receptor genes with altered expression in S. litura resistant to Cry1Ca toxin. (C) 2015 Elsevier Inc. All rights reserved,

分类号： Q959.1

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