数字农科院2.0

Frequency detection of imidacloprid resistance allele in Aphis gossypii field populations by real-time PCR amplification of specific-allele (rtPASA)

文献类型： 外文期刊

作者： Cui, Li;Xu, Xibao;Rui, Changhui

作者机构：

关键词： Aphis gossypii;Imidacloprid resistance;Real-time PASA (rtPASA);cPASA;Allele frequency

期刊名称： PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

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收录情况： SCI

摘要： The Aphis gossypii Glover (Hemiptera: Aphididae) is one of the most serious pests worldwide, and imidacloprid has been widely used to control this insect pest. Just like other classes of insecticides, the resistance to imidacloprid has been found in A. gossypii. An amino acid mutation (R81T) in the nicotinic acetylcholine receptor (nAChR) betal subunit was detected in the imidacloprid-resistant A. gossypii collected from Langfang (LF) and Dezhou (DZ) cities. To estimate the R81T mutation frequency of A. gossypii field populations, a simple, rapid and accurate rtPASA (real-time PCR amplification of specific allele) protocol was developed. The performance of the rtPASA protocol was evaluated by comparing with the data generated by a cPASA (competitive PCR amplification of specific allele) method from 50 individual genotypes. The R81T allele frequencies of the LF population (34.7% +/- 1.3%) and DZ population (45.2% +/- 5.2%) estimated by the rtPASA protocol matched the frequencies (LF 38.1%, DZ 48.2%) deduced by the cPASA method in specimens. The results indicated that the rtPASA format was applicable for the detection of mutation associated with imidacloprid resistance and will allow rapid and efficient monitoring of A. gossypii resistance in field populations in a high throughput format. (C) 2015 Elsevier Inc. All rights reserved.

分类号： S4

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